Functional Compounds of Cold-Pressed Pomegranate Seed Oil: Fatty Acids and Phytosterols Profile as Quality Biomarkers for Origin Discrimination

Iriti, Giuseppe; Bonacci, Sonia; Lopreiato, Vincenzo; Frisina, Marialaura; Oliverio, Manuela; Procopio, Antonio

doi:10.3390/foods12132599

Cited by 8 publications

(7 citation statements)

References 40 publications

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“…Total polyphenols were quantitatively measured by Folin-Ciocalteu spectrophotometric assay as reported by Iriti et al with some modifications [29]. Briefly, 5 g of oil samples were solubilized in 5 mL of hexane and extracted in a separating funnel with 10 mL of methanol/water (80:20, v/v) three times.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim

Frisina,

Bonacci,

Oliverio

et al. 2023

Foods

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The beneficial properties of extra virgin olive oil (EVOO) on lipids blood levels were recognized by the European Food Safety Authority (EFSA) with a health claim, specifically referring to EVOOs containing at least 5 mg of hydroxytyrosol and its secoiridoids derivatives per 20 g of oil. The main purpose of the work was to characterize the phenolic profile of two commercially available Calabrian monovarietal EVOOs (Nocellara del Belice, VN; Dolce di Rossano, VDR), and to study the effect of one-year storage on secoiridoids composition, by monthly controls. A new UHPLC-ESI-HRMS method was developed and validated, thus facilitating the EFSA claim application and allowing producers to valorize their products. Seven biologically active compounds were chosen: tyrosol, hydroxytyrosol, oleocanthal, oleacein, oleuropein aglycone, verbascoside, and oleuropein. LODs and LOQs were 0.001–0.02 mg g−1 and 0.002–0.08 mg g−1, respectively. The variation coefficients were ≤20% and the percentage of recovery was between 89–109%. During the 12-month storage period, the concentration of selected compounds ranged between 1258.78–1478.91 mg Kg−1 for VN, and 1408.22–2071.45 mg Kg−1 for VDR, with a decrease of 15% and 32% respectively. The method allows an accurate quantification of EVOO phenols thus being useful to certify the nutraceutical properties of olive oil.

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Section: Total Phenolic Contentmentioning

confidence: 99%

Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim

Frisina,

Bonacci,

Oliverio

et al. 2023

Foods

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“…Moreover, pomegranate seed oil, which is primarily composed of punicic acid, possesses strong anti-inflammatory and antioxidant properties [ 39 ]. Its high radical scavenging activity as a result of the presence of natural antioxidants including tocopherols makes it an excellent source of these beneficial compounds [ 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Design of Physicochemical Properties of Eggs as a Result of Modification of the Fat Fraction of Laying Feed

Filipiak-Florkiewicz,

Dymińska-Czyż,

Szymczyk

et al. 2024

Molecules

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The aim of this study was to investigate and confirm the properties of eggs produced by laying hens fed a diet consisting of pomegranate seed oil as a source of CLnA and flaxseed oil as a source of α-linolenic acid. The study involved determining the chemical composition of the eggs, including their fatty acid profile. The results showed that modifying the laying hens’ feed composition resulted in eggs with high nutritional value, with a favorable change in their fatty acid profile. In most cases, the addition of linseed oil or pomegranate seed oil did not affect the physical and chemical properties of the eggs. However, the diet of laying hens had a positive effect on the fatty acid profile of the egg yolk. The presence of conjugated linolenic acid trienes in eggs produced by laying hens fed a modified diet makes them a potential source of these compounds for human consumption.

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“…The consumption of this fruit and its juice is gaining worldwide popularity not just for its unique color and taste but also because it is reported to be rich in bioactive compounds with beneficial effects in relation to several diseases, such as inflammatory pathologies, cancer, diabetes, vascular diseases, osteoporosis, and bacterial and viral infections [2][3][4][5][6][7][8][9]. In the recent years, pomegranate seeds have been recommended as a food supplement or in cosmetic products [10][11][12][13][14]. However, sometimes its consumption has to be avoided because this fruit is also a source of molecules which can trigger allergic reactions.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and IgE Binding of Two 2S Albumins of Pomegranate Seeds

Tuppo,

Alessandri,

Zaccaro

et al. 2024

Foods

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Literature reports suggest that the presence of proteins in pomegranate seeds is responsible for sensitization and IgE-mediated allergic reactions. The objective of this study was the analysis of a pomegranate seed extract and the isolation and characterization of proteins contained in high amounts. The extract characterization showed a protein profile with main bands at about 18 kDa and below 10 kDa upon SDS-PAGE, and molecules were recognized by specific IgEs upon immunoblotting. Then, two new 2S albumins, a monomeric and a heterodimeric one, were isolated by using classical biochemical methods. They were identified via direct protein sequencing and mass spectrometry, and their primary structure was analyzed and compared with homologous allergenic proteins via bioinformatics. In an Italian population of 703 suspected allergic patients, analyzed by using the FABER® test, the frequency of sensitization to the monomeric and heterodimeric 2S albumins was 1.7% and 0.28%, respectively. This study reports for the first time the isolation and characterization of two 2S albumins from pomegranate seeds. The clinical relevance of these molecules needs further investigation, for instance in populations having different exposures and allergy profiles.

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Functional Compounds of Cold-Pressed Pomegranate Seed Oil: Fatty Acids and Phytosterols Profile as Quality Biomarkers for Origin Discrimination

Cited by 8 publications

References 40 publications

Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim

Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim

Design of Physicochemical Properties of Eggs as a Result of Modification of the Fat Fraction of Laying Feed

Isolation, Characterization and IgE Binding of Two 2S Albumins of Pomegranate Seeds

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