Functional connectivity fMRI in mouse brain at 7T using isoflurane

Guilfoyle, David N.; Gerum, Scott; Sanchez, Jamie L.; Balla, Andrea; Sershen, Henry; Javitt, Daniel C.; Hoptman, Matthew J.

doi:10.1016/j.jneumeth.2013.01.019

Cited by 53 publications

(38 citation statements)

References 26 publications

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“…The most commonly observed cortical RSNs are the somatosensory cortex, motor cortex, cingulate/retrosplenial cortex, and piriform cortex, while the most commonly observed subcortical RSNs are the hippocampus, striatum (espcially caudate-putamen), thalamus and hypothalamus (Jonckers et al, 2011;Grandjean et al, 2014b;Mechling et al, 2014;Nasrallah et al, 2014a;Sforazzini et al, 2014;. The presence of a default-mode like network was reported by some studies using different anaesthetic regimens (Guilfoyle et al, 2013;Sforazzini et al, 2014;Stafford et al, 2014;Liska et al, 2015;Zerbi et al, 2015). Topological analysis of the functional brain network in mice revealed that the mouse brain is organized into segregated modules and preserves small-worldness under anaesthesia.…”

Section: 24c Current Knowledge Of Functional Network and Networkmentioning

confidence: 90%

“…However, higher fields and small brain size cause other issues (Baltes et al, 2011). For example, more severe image distortions due to increased susceptibility artifacts at larger tissue-bone and tissue-air interfaces of the mouse brain, lower local field homogeneity due to generally poorer shimming capability at high fields, and reduced detection sensitivity (Nair and Duong, 2004;Baltes et al, 2011;Guilfoyle et al, 2013;Nasrallah et al, 2014a). A cryogenic radiofrequency probe (CryoProbe) is one strategy to address these problems, which has been shown to achieve enhanced sensitivity to the mouse BOLD-based fMRI signal (Baltes et al, 2011).…”

Section: Data Acquisition Of Mouse Rs-fmrimentioning

confidence: 99%

“…FC studies using mouse rs-fMRI showed that long-range connectivity in the striatum was suppressed, but other resting-state networks were detectable at a low dosage level under isoflurane anaesthesia (Grandjean et al, 2014b). Moreover, effective electrical stimulation, pharmacological stimulation and resting-state studies all prove it to be a very useful anaesthetic agent on mouse fMRI studies (Mueggler et al, 2002;Bosshard et al, 2010;Guilfoyle et al, 2013).…”

Section: General Anaesthesia and Commonly Used Anaesthetic Regimens Fmentioning

confidence: 99%

“…A major feature of rodent functional imaging has been the use of anesthesia, which eases the restrain on the subjects, and controls stress levels, while mouse awake imaging with fMRI was reported impracticable . Previous studies repeatedly observed several resting-state functional networks (RSNs) in mice under different anaesthetic regimen (Jonckers et al, 2011;Guilfoyle et al, 2013;Grandjean et al, 2014b;Mechling et al, 2014;Nasrallah et al, 2014a;Sforazzini et al, 2014;Liska et al, 2015). Comparative studies on rodents reported agent-specific characteristics in functional connectivity (FC) patterns (Williams et al, 2010;Grandjean et al, 2014b;Jonckers et al, 2014).…”

Section: Introductionmentioning

confidence: 97%

“…The reasons are mainly due to technical and physiological issues, including low SNR because of the high magnetic field of small animal scanners, application of anaesthesia and tight control of physiological states of the mouse during scanning (Benveniste and Blackband, 2006;Guilfoyle et al, 2013;Nasrallah et al, 2014a). Recent progress in mouse rs-fMRI includes studies on the detectable RSNs in healthy mice under anaesthesia (Jonckers et al, 2011;Guilfoyle et al, 2013;Grandjean et al, 2014b;Mechling et al, 2014;Nasrallah et al, 2014a;Sforazzini et al, 2014;Stafford et al, 2014;Zerbi et al, 2015), some network topological properties observed in mice (Mechling et al, 2014;Liska et al, 2015), comparative studies on different anaesthetic protocols (Grandjean et al, 2014b;Jonckers et al, 2014), and studies on mouse models of some brain disorders (Shah et al, 2013;Grandjean et al, 2014c;Zerbi et al, 2014;Haberl et al, 2015;Grandjean et al, 2016;Sforazzini et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Functional network study on the wild type and DISC1 transgenic mice

Wu¹

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Major mental disorders such as schizophrenia (SZ), bipolar disorder and major depression create a heavy social burden and remain poorly treated. Recent reports examining the genetic and molecular structure of brain disorders discovered the gene Disrupted-in-Schizophrenia 1 (DISC1) to be significantly associated with these disorders and plays a substantial role in influencing a range of shared endophenotypes underlying these disorders. This has led to a powerful gateway to explore the underlying pathology and to achieve better diagnosis and treatments of major mental diseases. Characterising the phenotypes of DISC1 in brain functions would provide valuable insights into the mechanism of how this gene influences the brain.Resting-state fMRI (rs-fMRI) is a powerful technique being applied to more than 30 different kinds of brain disorders and different species. Findings from rs-fMRI studies on humans provide ample evidence of abnormal functional patterns in patients with mental illnesses. Specifically, altered functional connectivity (FC) and network topologies are proposed to be plausible imaging endophenotypes of psychotic disorders. However, little is known about the direct effects of gene dysfunction, such as DISC1 mutations, on restingstate brain activations. One aim of this thesis is to investigate the impacts of DISC1 on variations of resting-state functional networks (RSNs) in anaesthetized mouse brains using DISC1 transgenic mice and rs-fMRI.Before characterizing disease phenotypes in mouse models, it is necessary to understand how the mouse brain functions in normal states. Anaesthesia is currently an integrated part of most mouse rs-fMRI experiment. However, it influences blood-oxygenation-leveldependent (BOLD) fMRI signals via modulation of neurovascular coupling and brain metabolism, and therefore alters FC and topologies of RSNs. Investigating FC and its changes associated with genes therefore initially requires an understanding of the anaesthetics. Furthermore, various anaesthetic protocols are adopted by different labs leading to difficulties in result comparisons. The brain is a complex and hierarchical system, whereby its RSN architecture can be characterised from mutiple perspectives at regional or global levels. Previous mouse rs-fMRI studies have focused on long-distance FC, however, given the non-uniform influence of anaesthesia on the brain, mapping functional activations across scales in mice under different anaesthetics remain sparsely explored. Establishing this relationahsip would provide a reliable reference to study mouse brain functions in abnormal states and facilitate comparisons across laboratories. | P a g eIn the first aim of this thesis, I investigated the local connectivity in wide type (WT) mice under six commonly used anaesthetic regimens. I identified that the regional connectivity altered across the brain as a function of the expression density of receptors relative to specific agents. In addition, the depth of anaesthesia influences local synchronization.In the seco...

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Section: 24c Current Knowledge Of Functional Network and Networkmentioning

confidence: 90%

Section: Data Acquisition Of Mouse Rs-fmrimentioning

confidence: 99%

Section: General Anaesthesia and Commonly Used Anaesthetic Regimens Fmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Functional network study on the wild type and DISC1 transgenic mice

Wu¹

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Mapping the Connectional Architecture of the Rodent Brain with fMRI

Schwarz

Gozzi

2017

Handbook of Neurobehavioral Genetics and Phenotyping

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Resting State fMRI in Rodents

et al. 2018

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Resting state functional MRI (fMRI) and functional connectivity are widely applied in humans to examine the role of brain networks in normal function and dysfunction. A similar approach can be taken in rodents, either to obtain translational measures in models of brain disorders or to more carefully examine the neurophysiological underpinnings of the networks. A protocol for resting state functional connectivity in the anesthetized rat, from animal setup to data acquisition to possible pipelines for data analysis, is described. © 2018 by John Wiley & Sons, Inc.

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Functional connectivity fMRI in mouse brain at 7T using isoflurane

Cited by 53 publications

References 26 publications

Functional network study on the wild type and DISC1 transgenic mice

Functional network study on the wild type and DISC1 transgenic mice

Mapping the Connectional Architecture of the Rodent Brain with fMRI

Resting State fMRI in Rodents

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