Functional conservation of suppressors of cytokine signaling proteins between teleosts and mammals: Atlantic salmon SOCS1 binds to JAK/STAT family members and suppresses type I and II IFN signaling

Skjesol, Astrid; Liebe, Theresa; Iliev, Dimitar B.; Thomassen, Ernst Ivan Simon; Tollersrud, Linn Greiner; Sobhkhez, Mehrdad; Joensen, Lisbeth Lindenskov; Secombes, Christopher J.; Jørgensen, Jorunn B.

doi:10.1016/j.dci.2014.02.009

Cited by 64 publications

(41 citation statements)

References 54 publications

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“…of SOCS has also been reported in fish models, including salmon (Skjesol et al 2014), yellow perch (Shepherd et al 2012), rainbow trout (Wang & Secombes 2008, Wang et al 2010, puffer fish (Jin et al 2007), and turbot (Zhang et al 2011). Similar to grass carp, SOCS expression was found to be widely expressed, e.g., in the gills, spleen, gut, liver, kidney, muscle, and brain.…”

Section: Discussionmentioning

confidence: 86%

“…In recent years, SOCS proteins have been cloned in fish models, e.g., trout (Wang et al 2010, Maehr et al 2014, salmon (Skjesol et al 2014), catfish (Yao et al 2015), turbot (Zhang et al 2011), and zebrafish (Wang et al 2011). Data mining of genome databases not only reveals the orthologues of mammalian SOCS but also reveals new paralogues of Cish, Socs3, and Socs5 unique to fish species (Jin et al 2008, Wang et al 2011, which are presumably the result of fish-specific 3R genome duplication (Kassahn et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Type II SOCS as a feedback repressor for GH-induced Igf1 expression in carp hepatocytes

Jiang

Jia

et al. 2016

Journal of Endocrinology

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Type II suppressor of cytokine signaling (SOCS) serve as feedback repressors for cytokines and are known to inhibit growth hormone (GH) actions. However, direct evidence for SOCS modulation of GH-induced insulin-like growth factor 1 (Igf1) expression is lacking, and the post-receptor signaling for SOCS expression at the hepatic level is still unclear. To shed light on the comparative aspects of SOCS in GH functions, grass carp was used as a model to study the role of type II SOCS in GH-induced Igf1 expression. Structural identity of type II SOCS, Socs1-3 and cytokine-inducible SH2-containing protein (Cish), was established in grass carp by 5'/3'-RACE, and their expression at both transcript and protein levels were confirmed in the liver by RT-PCR and LC/MS/MS respectively. In carp hepatocytes, GH treatment induced rapid phosphorylation of JAK 2 , STATs, MAPK, PI3K, and protein kinase B (Akt) with parallel rises in socs1-3 and cish mRNA levels, and these stimulatory effects on type II SOCS were shown to occur before the gradual loss of igf1 gene expression caused by prolonged exposure of GH. Furthermore, GH-induced type II SOCS gene expression could be negated by inhibiting JAK 2 , STATs, MEK 1/2 , P 38 MAPK , PI3K, and/or Akt respectively. In CHO cells transfected with carp GH receptor, over-expression of these newly cloned type II SOCS not only suppressed JAK 2 /STAT 5 signaling with GH treatment but also inhibited GH-induced grass carp Igf1 promoter activity. These results, taken together, suggest that type II SOCS could be induced by GH in the carp liver via JAK 2 /STATs, MAPK, and PI3K/Akt cascades and serve as feedback repressors for GH signaling and induction of igf1 gene expression.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Type II SOCS as a feedback repressor for GH-induced Igf1 expression in carp hepatocytes

Jiang

Jia

et al. 2016

Journal of Endocrinology

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“…Recent results have shown that there is a conserved intracellular dsRNA-mediated RLR-IFN pathway in fish Zhang et al, 2014) and fish IFNs exert their effects through the conserved Jak-Stat pathway (Shi et al, 2012;Skjesol et al, 2010Skjesol et al, , 2014Yu et al, 2010). As fish USP18 was significantly induced by IFN and IFN stimuli (Fig.…”

Section: Regulation Of Fish Usp18 Expression Via Rlr-ifn Signaling Pamentioning

confidence: 90%

“…Fish IFNs exert their function by inducing the expression of downstream ISGs through Jak-Stat signaling pathway (Altmann et al, 2003;Shi et al, 2012;Skjesol et al, 2010Skjesol et al, , 2014Yu et al, 2010). In this study, we reported identification and characterization of fish USP18 homologous genes.…”

Section: Introductionmentioning

confidence: 94%

Expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (USP18) genes

Chen¹,

Zhang²,

Gui³

2015

Developmental & Comparative Immunology

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“…Recently, a SOCS1 gene has been identified in several teleost species [18,19] . In vitro studies also suggest a possible role of SOCS1 in fish immunity [20,21] . However, the limited information on SOCS1 from non-mammalian vertebrates greatly limits our understanding of the conserved physiological roles of SOCS1 across vertebrates.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of two suppressor of cytokine signaling 1 genes (SOCS1a and SOCS1b) in chickens

Xue¹,

Zhang²,

Li³

et al. 2015

Front. Agr. Sci. Eng.

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Suppressor of cytokine signaling 1 (SOCS1) protein can inhibit the signal transduction triggered by some cytokines or hormones and thus are important in many physiological/pathological processes, including innate and adaptive immunity, inflammation, and development in mammals. However, there is sparse information about their structure, tissue expression, in birds, where their biological functions remain unknown. In this study, we cloned and characterized two SOCS1 genes (named cSOCS1a and cSOCS1b) from chickens. SOCS1a is predicted to encode a 207-amino acid protein, which shares high amino acid sequence identity (64%-67%) with human and mouse SOCS1. Besides SOCS1a, a novel SOCS1b gene was also identified in chickens and other non-mammalian vertebrates including Xenopus tropicalis. Chicken SOCS1b is predicted to encode a 212-amino acid protein, which shares only 30%-32% amino acid sequence identity with human SOCS1 and cSOCS1a. RT-PCR assay revealed that both cSOCS1a and cSOCS1b are widely expressed in all chicken tissues. Using a luciferase reporter assay system, we further demonstrated that transient expression of cSOCS1a and cSOCS1b can significantly inhibit chicken growth hormone (GH)-or prolactin (PRL)-induced luciferase activities of Hep G2 cells expressing cGH receptor (or cPRL receptor), indicating that SOCS1a and SOCS1b proteins can negatively regulate GH/PRL signaling. Taken together, these data suggest that both cSOCS1a and cSOCS1b may function as negative regulators of cytokine/hormone actions, such as modulation of GH/PRL actions in chickens.

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Functional conservation of suppressors of cytokine signaling proteins between teleosts and mammals: Atlantic salmon SOCS1 binds to JAK/STAT family members and suppresses type I and II IFN signaling

Cited by 64 publications

References 54 publications

Type II SOCS as a feedback repressor for GH-induced Igf1 expression in carp hepatocytes

Type II SOCS as a feedback repressor for GH-induced Igf1 expression in carp hepatocytes

Expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (USP18) genes

Molecular characterization of two suppressor of cytokine signaling 1 genes (SOCS1a and SOCS1b) in chickens

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