Functional Cooperation of Simian Virus 40 Promoter Factor 1 and CCAAT/Enhancer-Binding Protein β and δ in Lipopolysaccharide-Induced Gene Activation of IL-10 in Mouse Macrophages

Liu, Yi Wen; Tseng, Hui Ping; Chen, Lei Chin; Chen, Ben Kuen; Chang, Wen Chang

doi:10.4049/jimmunol.171.2.821

Cited by 98 publications

(86 citation statements)

References 47 publications

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“…The protein interaction between Sp1 and C/EBPb or between Sp1 and C/EBPd upon the LPS treatment was also confirmed by coimmunoprecipation [45]. In the present study, we studied further the interaction between C/EBPd and Sp1, mapped the proteinprotein interaction domains, and found that the major interaction domains of C/EBPd and Sp1 were located in their C-termini.…”

mentioning

confidence: 85%

“…Cells were transfected with 0.2 lg of luciferase reporter plasmid, 0.8 lg of pPac-Sp1, and 0.4 lg of pPac-C/EBPd for 48 h, and then luciferase activity assay was performed as previous report [45]. Variable amounts of expression plasmids were compensated with the empty vector pPac.…”

Section: Transfection Of Sl2 Cells By Calcium Phosphate Methodsmentioning

confidence: 99%

“…In a previous study [45], we reported that lipopolysaccharide (LPS) activates the transcription of the IL-10 gene promoter through the Sp1 and C/EBP binding sites, and that Sp1, C/EBPb or C/EBPd could coactivate IL-10 gene expression in mouse macrophage cells. The protein interaction between Sp1 and C/EBPb or between Sp1 and C/EBPd upon the LPS treatment was also confirmed by coimmunoprecipation [45].…”

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Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage

et al. 2006

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SummaryWe previously reported that LPS activates the transcription of the IL-10 gene through the Sp1 and C/EBP binding sites and indicated that Sp1, C/EBPb and C/EBPd can coactivate the IL-10 gene expression in mouse macrophage cells [Liu Y.-W., Tseng H.-P., Chen L.-C., Chen B.-K., Chang W.-C. J. Immunol. 171: 821-828, 2003]. In the present report, we demonstrated the direct physical interaction between C/EBPd and Sp1, and also mapped the interaction domains of these two proteins. C/EBPd binds to Sp1 via its basic region leucine zipper domain. The C-terminus of Sp1 was also the major region interacting with C/EBPd. However, both glutamine-and serine/threonine-rich homologus regions of Sp1 also interacted with C/ EBPd. The binding of Sp1 and C/EBPd as a complex to the Sp1 binding site on the promoter of IL-10 was further confirmed by using the DNA affinity precipitation assay. By using Sp1-deficient SL2 cells, we also found that the overexpressions of C/EBPd and Sp1 synergically activate the transcriptional activity of IL-10 gene. Taken together, our present results revealed a novel mechanism of a superactivation of Sp1 by C/ EBPd via a direct interaction between these two transcription factors leading to the activation of the IL-10 gene in mouse macrophage cells.

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confidence: 85%

Section: Transfection Of Sl2 Cells By Calcium Phosphate Methodsmentioning

confidence: 99%

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confidence: 99%

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Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage

et al. 2006

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“…In earlier studies, LPS has been shown to activate IL-10 gene expression through Sp1. 34,35 Brightbill et al 35 showed that transfection of a murine macrophage cell line with the IL-10 promoter construct resulted in an eightfold increase on LPS stimulation. These results are in line with the results presented in the current study.…”

Section: Sp1 Transcription Factor Binds To the Il-10 à1087 G-allele Lmentioning

confidence: 99%

The Sp1 transcription factor binds to the G-allele of the –1087 IL-10 gene polymorphism and enhances transcriptional activation

et al. 2008

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The objectives of this study were to evaluate the influence of the À1087 single nucleotide polymorphism (SNP) on the gene expression of interleukin (IL)-10 and to identify transcription factors binding to this site in B cells. Using electrophoretic mobilityshift assays and nuclear extract from the DG75 B-cell line, we demonstrated that the Sp1 transcription factor bound to the À1087 G-allele of the IL-10 promoter and that the transcription factors PU.1 and Spi-B bound to both the G-and A-alleles. Transient transfections showed that lipopolysaccharide stimulation resulted in a 15-fold increase in promoter activity for the G-allele as compared to a 6-fold increase for the A-allele. Co-transfection with Sp1 expression vector in Sp1-deficient SL2 cells leading to Sp1 binding to the G-allele of the À1087 SNP resulted in increased IL-10 promoter activity. The results suggest a role for Sp1 transcription factor in the activation of IL-10 through the G-allele of the À1087 SNP in response to inflammatory signals.

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“…Candidate regulators can be identified by analysis of promoters of M2-specific genes in macrophages, and among these, the Il10 and Arg-1 promoters are regulated by C/EBP␤ (9,10). C/EBP␤ is a member of the C/EBP family of basic region-leucine zipper (bZIP) proteins and is known to be important for the antibacterial activity of macrophages (11).…”

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A CREB-C/EBPβ cascade induces M2 macrophage-specific gene expression and promotes muscle injury repair

Ruffell

Mourkioti²,

Gambardella³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

549

439

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Macrophages play an essential role in the resolution of tissue damage through removal of necrotic cells, thus paving the way for tissue regeneration. Macrophages also directly support the formation of new tissue to replace the injury, through their acquisition of an anti-inflammatory, or M2, phenotype, characterized by a gene expression program that includes IL-10, the IL-13 receptor, and arginase 1. We report that deletion of two CREB-binding sites from the Cebpb promoter abrogates Cebpb induction upon macrophage activation. This blocks the downstream induction of M2-specific Msr1, Il10, II13ra, and Arg-1 genes, whereas the inflammatory (M1) genes Il1, Il6, Tnfa, and Il12 are not affected. Mice carrying the mutated Cebpb promoter (␤⌬Cre) remove necrotic tissue from injured muscle, but exhibit severe defects in muscle fiber regeneration. Conditional deletion of the Cebpb gene in muscle cells does not affect regeneration, showing that the C/EBP␤ cascade leading to muscle repair is muscle-extrinsic. While ␤⌬Cre macrophages efficiently infiltrate injured muscle they fail to upregulate Cebpb, leading to decreased Arg-1 expression. CREB-mediated induction of Cebpb expression is therefore required in infiltrating macrophages for upregulation of M2-specific genes and muscle regeneration, providing a direct genetic link between these two processes.macrophage polarization ͉ muscle regeneration ͉ transcription

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Functional Cooperation of Simian Virus 40 Promoter Factor 1 and CCAAT/Enhancer-Binding Protein β and δ in Lipopolysaccharide-Induced Gene Activation of IL-10 in Mouse Macrophages

Cited by 98 publications

References 47 publications

Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage

Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage

The Sp1 transcription factor binds to the G-allele of the –1087 IL-10 gene polymorphism and enhances transcriptional activation

A CREB-C/EBPβ cascade induces M2 macrophage-specific gene expression and promotes muscle injury repair

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