Functional Coordination among the Golgi Complex, the Centrosome and the Microtubule Cytoskeleton during the Cell Cycle

Mascanzoni, Fabiola; Iannitti, Roberta; Colanzi, Antonino

doi:10.3390/cells11030354

Cited by 22 publications

(22 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, the long-term functional implications of alterations of the mitotic inheritance of the GC are largely unexplored. This is a critical issue as the relocalization of a set of Golgi proteins is necessary for spindle formation (Altan-Bonnet et al, 2003;Mascanzoni et al, 2022). Therefore, errors of GC segregation could result in aberrant spindle formation, which can potentially alter the orientation of cell division, with deleterious consequences on tissue homeostasis, or cause errors of chromosome segregation, favoring cell transformation and tumorigenesis (Matthews et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…The structure and function of the GC are under the control of GTPases and several signaling pathways, including those involved in phosphoinositide metabolism and mitogenactivated protein kinase signaling (Kulkarni- Gosavi et al, 2019). Moreover, clustering and position of the stacks depend on the concerted actions of CE-and Golgi-nucleated microtubules (MTs) and actin cytoskeleton (Ravichandran et al, 2020;Mascanzoni et al, 2022). Despite this complexity, the GC can rapidly modify structure and localization in response to specific physiological conditions (Nakamura et al, 2012;Gosavi and Gleeson, 2017;Saraste and Prydz, 2019;Ravichandran et al, 2020).…”

Section: Structural Organization Of the Golgi Complexmentioning

confidence: 99%

See 1 more Smart Citation

Structural Organization and Function of the Golgi Ribbon During Cell Division

Ayala

Colanzi²

2022

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

The Golgi complex has a central role in the secretory traffic. In vertebrate cells it is generally organized in polarized stacks of cisternae that are laterally connected by membranous tubules, forming a structure known as Golgi ribbon. The steady state ribbon arrangement results from a dynamic equilibrium between formation and cleavage of the membrane tubules connecting the stacks. This balance is of great physiological relevance as the unlinking of the ribbon during G2 is required for mitotic entry. A block of this process induces a potent G2 arrest of the cell cycle, indicating that a mitotic “Golgi checkpoint” controls the correct pre-mitotic segregation of the Golgi ribbon. Then, after mitosis onset, the Golgi stacks undergo an extensive disassembly, which is necessary for proper spindle formation. Notably, several Golgi-associated proteins acquire new roles in spindle formation and mitotic progression during mitosis. Here we summarize the current knowledge about the basic principle of the Golgi architecture and its functional relationship with cell division to highlight crucial aspects that need to be addressed to help us understand the physiological significance of the ribbon and the pathological implications of alterations of this organization.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Structural Organization Of the Golgi Complexmentioning

confidence: 99%

Structural Organization and Function of the Golgi Ribbon During Cell Division

Ayala

Colanzi²

2022

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…GM130 activates the kinase YSK1 and the exchange factors TUBA and RasGFR to modulate cell polarity. In addition, the GM130/TPX2/Aurora-A pathway controls spindle formation and orientation [113]. The role of GM130 in tumorigenesis depends on the type of cancer.…”

Section: Gm130mentioning

confidence: 99%

Golgi Complex: A Signaling Hub in Cancer

Spano

Colanzi

2022

Cells

Self Cite

View full text Add to dashboard Cite

The Golgi Complex is the central hub in the endomembrane system and serves not only as a biosynthetic and processing center but also as a trafficking and sorting station for glycoproteins and lipids. In addition, it is an active signaling hub involved in the regulation of multiple cellular processes, including cell polarity, motility, growth, autophagy, apoptosis, inflammation, DNA repair and stress responses. As such, the dysregulation of the Golgi Complex-centered signaling cascades contributes to the onset of several pathological conditions, including cancer. This review summarizes the current knowledge on the signaling pathways regulated by the Golgi Complex and implicated in promoting cancer hallmarks and tumor progression.

show abstract

“…The Golgi apparatus has a characteristic structure in most eukaryotes, comprising a series of cisternae layered on top of each other to form the Golgi stack (Lowe, 2011; Shorter and Warren, 2002). In most vertebrate cells, the stacks are connected laterally to form the Golgi ribbon, which is usually positioned next to the centrosome by virtue of attachment to microtubules and the minus-end directed microtubule motor protein dynein (Brownhill et al, 2009; Mascanzoni et al, 2022; Thyberg and Moskalewski, 1999). The Golgi is a polarized structure, comprising an entry (cis-) face for cargo entering from the endoplasmic reticulum (Brandizzi and Barlowe, 2013), and an exit (trans-) face from where cargo is transported to various downstream destinations (Di Martino et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Vimentin intermediate filaments provide structural stability to the mammalian Golgi apparatus

Vitali

Witkos

Cutiongco

et al. 2022

Preprint

View full text Add to dashboard Cite

The Golgi apparatus comprises a connected ribbon of stacked cisternal membranes localized to the perinuclear region of most vertebrate cells. The position and morphology of this organelle depends upon interactions with microtubules and the actin cytoskeleton. In contrast, we know relatively little about the relationship of the Golgi apparatus with intermediate filaments. In this study we show that the Golgi is in close physical proximity to vimentin intermediate filaments (IFs) in cultured mouse and human cells. We also show that the trans-Golgi network coiled-coil protein GORAB can physically associate with IFs. Although loss of vimentin and/or GORAB does not have major effects upon Golgi morphology at steady-state, the Golgi undergoes more rapid disassembly upon chemical disruption with the drug brefeldin A, and slower reassembly upon drug washout, in vimentin knockout cells. Moreover, loss of vimentin causes reduced Golgi ribbon integrity when cells are cultured on high stiffness hydrogels, which is exacerbated by loss of GORAB. These results indicate that vimentin IFs contribute to the structural stability of the Golgi apparatus, and suggest a role for GORAB in this process.

show abstract

Functional Coordination among the Golgi Complex, the Centrosome and the Microtubule Cytoskeleton during the Cell Cycle

Cited by 22 publications

References 127 publications

Structural Organization and Function of the Golgi Ribbon During Cell Division

Structural Organization and Function of the Golgi Ribbon During Cell Division

Golgi Complex: A Signaling Hub in Cancer

Vimentin intermediate filaments provide structural stability to the mammalian Golgi apparatus

Contact Info

Product

Resources

About