Functional coupling of adenine nucleotide translocase and mitochondrial creatine kinase is enhanced after exercise training in lung transplant skeletal muscle

Abstract

Mechanisms responsible for limitation of exercise capacity in lung transplant recipients (LR) and benefits gained by exercise training were studied. Mitochondrial respiration parameters, energy transfer, and cell structure were assessed in vastus lateralis biopsies using the permeabilized fiber technique with histochemical and morphometric measurements. Twelve male controls (C) and 12 LR performed exercise training over 12 wk. Before exercise training, there were strong correlations between exercise capacity (maximal O(2) consumption and endurance time at 70% maximal power output) and cellular events, as assessed by percentage of type I fibers and apparent K(m) for exogenous ADP. Anticalcineurins were not involved in LR exercise limitation, since there were no differences in maximal mitochondrial rate of respiration before exercise training and no abnormalities in respiratory chain complexes compared with C. Training resulted in a significant increase in physiological parameters both at the cellular (apparent K(m) for exogenous ADP and stimulating effect of creatine) and integrated (maximal O(2) consumption, power output at ventilatory threshold, maximal power output, and endurance time at 70% maximal power output) levels in LR and C. After the training period, improvements in maximal O(2) consumption and in maximal mitochondrial rate of respiration were noted, as well as changes in endurance time and percentage of type I fibers. Because there were no changes in diameters and fiber types, baseline alteration of apparent K(m) for exogenous ADP and its improvement after training might be related to changes within the intracellular energetic units. After the training period, intracellular energetic units exhibited a higher control of mitochondrial respiration by creatine linked to a more efficient functional coupling adenine nucleotide translocase-mitochondrial creatine kinase, resulting in better exercise performances in C and LR.

A 9-wk docosahexaenoic acid-enriched supplementation improves endurance exercise capacity and skeletal muscle mitochondrial function in adult rats

“…We conclude that DHA has a specific effect on the regulation of the respiratory chain. This effect is quite similar to what is usually reported after endurance training (24,62). An increase in endurance capacity is not necessarily dependent solely on an increase in the V max of oxygen consumption.…”

Rehabilitation in Patients before and after Lung Transplantation

“…Three cohort studies demonstrated positive effects of exercise training on muscle function and exercise capacity in the long-term posttransplant phase [57,58,59]. Stiebellehner et al [59] studied the effects of 6 weeks of aerobic endurance exercise on a cycle ergometer in a group of 9 patients (average posttransplant time = 52 weeks).…”

Rehabilitation in Patients Undergoing Lung Transplantation (LTx)

“…7 There are also reports that exercise training has improved exercise capacity in lung transplant recipients (Table 1). [8][9][10][12][13][14] There were a variety of study designs, including one controlled trial and five prospective cohort studies. Outcome measures included spirometry six-minute walk test (6MWT), 10,14 quadriceps and handgrips force (QF and HGF), 9,10 health-related QOL, 14 maximal oxygen consumption (VO 2max ), and VO 2peak , 8,9,12 peak power output, 8 endurance time, 13 and additional measures of hemodynamic responses 12 to exercise and mitochondrial bioenergetics.…”

“…Outcome measures included spirometry six-minute walk test (6MWT), 10,14 quadriceps and handgrips force (QF and HGF), 9,10 health-related QOL, 14 maximal oxygen consumption (VO 2max ), and VO 2peak , 8,9,12 peak power output, 8 endurance time, 13 and additional measures of hemodynamic responses 12 to exercise and mitochondrial bioenergetics. 13 Six studies 8-10,12-14 included aerobic exercise, and two studies included both aerobic and resistance exercise. In these studies, exercise tolerance was improved.…”