Functional deficiencies in two distinct interferon <i>α</i>-producing cell populations in peripheral blood mononuclear cells from human immunodeficiency virus seropositive patients

Feldman, Stephen B.; Milone, Michael C.; Kloser, Patricia; Fitzgerald‐Bocarsly, Patricia

doi:10.1002/jlb.57.2.214

Cited by 41 publications

(41 citation statements)

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“…Human PDC constitute Ͻ1% of PBMC, making them difficult to isolate and study in large numbers. However, human PDC can be identified by using flow cytometry, allowing us to gate in on the small population of cells for both phenotypic and functional analysis (13). Human PDC have been characterized as cells that are lineage marker negative, HLA-DR ϩ , CD123 bright , and CD11c Ϫ (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The markedly lower frequency of SV-responsive IPC in monkeys compared to humans may reflect limitations to the ELISPOT assay. SV is known to induce both PDC and monocytes to produce IFN-␣, with the monocytes expressing 5-to 10-fold lower expression of IFN-␣ on a per-cell basis than the PDC (13,20). In the ELISPOT, this is seen by a mixture of small (monocyte-derived) and large (PDC-derived) spots.…”

Section: Discussionmentioning

confidence: 99%

“…ELISPOT assays for detection of IFN-␣-producing cells were carried out as previously described (13). Briefly, PBMC (10 6 /ml) were either mock stimulated (placed in the incubator with no virus) or stimulated for 6 h with HSV-1 at an multiplicity of infection of 1.…”

Section: Vol 12 2005 Characterization Of Macaque Pdc 427mentioning

confidence: 99%

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Characterization of Virus-Responsive Plasmacytoid Dendritic Cells in the Rhesus Macaque

Chung

Amrute

Abel

et al. 2005

Clin Vaccine Immunol

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Plasmacytoid dendritic cells (PDC) are potent producers of alpha interferon (IFN-␣In this study, we demonstrate that peripheral blood PDC from healthy macaques are both phenotypically and functionally similar to human PDC and that reagents used for human studies can be used to study macaque PDC. Both human and macaque PBMC expressed IFN-␣ in response to herpes simplex virus (HSV), the prototypical activator of PDC, as measured by using an IFN bioassay and IFN-␣-specific enzyme-linked immunospot assays. Similar to human PDC, macaque PDC were identified by using flow cytometry as CD123 ؉ HLA-DR ؉ lineage ؊ cells. In addition, like human PDC, macaque PDC expressed intracellular IFN-␣, tumor necrosis factor alpha, macrophage inflammatory protein 1␤/CCL4, and IFN-inducible protein 10/CXCL10 upon stimulation with HSV, all as determined by intracellular flow cytometry. We found that IFN regulatory factor 7, which is required for the expression of IFN-␣ genes, was, similar to human PDC, expressed at high levels in macaque PDC compared to monocytes and CD8 ؉ T cells. These findings establish the phenotypic and functional similarity of human and macaque PDC and confirm the utility of tools developed for studying human PDC in this animal model.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of Virus-Responsive Plasmacytoid Dendritic Cells in the Rhesus Macaque

Chung

Amrute

Abel

et al. 2005

Clin Vaccine Immunol

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“…In other studies, the defect in ex vivo production of IFN-a in HIV-1-infected patients has been described but its mechanism remains unclear [12][13][14][15][16][17]. This is the first report of an association between the profile of T-cell-derived cytokines and the production of IFN-a in HIV-1 infection.…”

Section: Discussionmentioning

confidence: 96%

Production of Interleukin‐4, Interferon (IFN)‐γ and IFN‐α in Human Immunodeficiency Virus‐1 Infection: An Imbalance of Type 1 and Type 2 Cytokines may Reduce the Synthesis of IFN‐α

Hober¹,

Benyoucef²,

Chehadeh³

et al. 1998

Scand J Immunol

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Interferon‐α (IFN‐α) is an important molecule in the antiviral response, but cells from HIV‐1‐infected individuals show a reduced ability to secrete IFN‐α. We investigated an association between an imbalance of type 1/type 2 cytokines and the production of IFN‐α in HIV‐1 infection. We used whole blood culture to study the cytokine production profile, interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4), in response to HIV‐1 antigens and to study the Sendai Virus and HSV‐1‐induced‐production of IFN‐α in seven HIV‐1‐infected patients. An impaired synthesis of IFN‐α was obtained in patients with a predominant IL‐4 production (IL‐4 > IFN‐γ), and we found a positive correlation between the ex vivo production of IFN‐α and the IFN‐γ/IL‐4 ratio but not with the HIV RNA copy number in plasma. We investigated the role of T‐cell‐derived cytokines in the in vitro production of IFN‐α by PBMC from eight healthy donors, activated with Sendai Virus or HSV‐1. Whereas type 2 cytokines (IL‐4, IL‐13) inhibited virus‐induced IFN‐α synthesis, on the contrary, type 1 cytokines (IL‐2, IFN‐γ) enhanced it. A disarray in the T‐cell‐derived cytokine response may play a role in the defect of IFN‐α production in HIV‐1‐infected individuals. Further investigations are needed to explore this hypothesis.

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“…While mDCs are present in virtually all organs 21 and perform virtually all tasks involved in triggering immune response, 21,22 pDCs constitute a unique cell population comprising only 0.2-0.8% of all peripheral blood mononuclear cells (PBMCs). 23,24 The pDCs express surface molecules CD4, CD45RA, a-chain of IL-3 and Major Histocompatibility Complex (MHC II) molecules (HLA-DRII), but lack typical markers of other common cell lineages, CD3, CD11c, CD11b, CD14, CD16, CD19 and CD56. Additionally, the pDCs express specific markers such as CD303 (BDCA-2-type II transmembrane glycoprotein-C-type lectin) and CD304 (BDCA-4-neuropilin 1).…”

Section: Introductionmentioning

confidence: 99%

A correlation of measles specific antibodies and the number of plasmacytoid dendritic cells is observed after measles vaccination in 9 month old infants

García‐León

Bonifaz

Espinosa-Torres

et al. 2015

Human Vaccines & Immunotherapeutics

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Measles virus (MeV) represents one of the main causes of death among young children, particularly in developing countries. Upon infection, MeV controls both interferon induction (IFN) and the interferon signaling pathway which results in a severe host immunosuppression that can persists for up to 6 mo after infection. Despite the global biology of MeV infection is well studied, the role of the plasmacytoid dendritic cells (pDCs) during the host innate immune response after measles vaccination remains largely uncharacterized. Here we investigated the role of pDCs, the major producers of interferon in response to viral infections, in the development of adaptive immune response against MeV vaccine. We report that there is a strong correlation between pDCs population and the humoral immune response to Edmonston Zagreb (EZ) measles vaccination in 9-month-old mexican infants. Five infants were further evaluated after vaccination, showing a clear increase in pDCs at baseline, one week and 3 months after immunization. Three months postvaccination they showed increase in memory T-cells and pDCs populations, high induction of adaptive immunity and also observed a correlation between pDCs number and the humoral immune response. These findings suggest that the development and magnitude of the adaptive immune response following measles immunization is directly dependent on the number of pDCs of the innate immune response.

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Functional deficiencies in two distinct interferon α-producing cell populations in peripheral blood mononuclear cells from human immunodeficiency virus seropositive patients

Cited by 41 publications

References 0 publications

Characterization of Virus-Responsive Plasmacytoid Dendritic Cells in the Rhesus Macaque

Characterization of Virus-Responsive Plasmacytoid Dendritic Cells in the Rhesus Macaque

Production of Interleukin‐4, Interferon (IFN)‐γ and IFN‐α in Human Immunodeficiency Virus‐1 Infection: An Imbalance of Type 1 and Type 2 Cytokines may Reduce the Synthesis of IFN‐α

A correlation of measles specific antibodies and the number of plasmacytoid dendritic cells is observed after measles vaccination in 9 month old infants

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