Functional Development of the Small Intestine of Japanese Quail Hatched on Mir Orbital Station

Lenhardt, L; Cigánková, Viera; Zibrín, M; Kocisová, J; Tomková, I.; Sabo, V.; Bodă, K; Dadasheva, O. A.; Gurieva, T.S.; Mózeš, Š

doi:10.2754/avb200170020127

Cited by 8 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The adaptive features, including different mechanisms from molecular pathways to cognitive and behavioral adaptations, to the space condition of the exact species are determined by the biology and phylogenetic history of the species [6]. There have also been numerous studies on fish models [7,8,9,10,11,12]; amphibians, such as clawed frogs [13] and ribbed newts [14,15,16,17,18]; and birds (Japanese quail) [19,20,21,22,23,24]. Reptiles are rare model objects for space and microgravity research [25,26,27,28,29,30,31,32,33,34] (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Reptiles in Space Missions: Results and Perspectives

Gulimova

Proshchina

Kharlamova

et al. 2019

IJMS

View full text Add to dashboard Cite

Reptiles are a rare model object for space research. However, some reptile species demonstrate effective adaptation to spaceflight conditions. The main scope of this review is a comparative analysis of reptile experimental exposure in weightlessness, demonstrating the advantages and shortcomings of this model. The description of the known reptile experiments using turtles and geckos in the space and parabolic flight experiments is provided. Behavior, skeletal bones (morphology, histology, and X-ray microtomography), internal organs, and the nervous system (morphology, histology, and immunohistochemistry) are studied in the spaceflight experiments to date, while molecular and physiological results are restricted. Therefore, the results are discussed in the scope of molecular data collected from mammalian (mainly rodents) specimens and cell cultures in the parabolic and orbital flights and simulated microgravity. The published data are compared with the results of the gecko model studies after the 12–44.5-day spaceflights with special reference to the unique peculiarities of the gecko model for the orbital experiments. The complex study of thick-toed geckos after three spaceflights, in which all geckos survived and demonstrated effective adaptation to spaceflight conditions, was performed. However, future investigations are needed to study molecular mechanisms of gecko adaptation in space.

show abstract

Section: Introductionmentioning

confidence: 99%

Reptiles in Space Missions: Results and Perspectives

Gulimova

Proshchina

Kharlamova

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Due to the lack of gravity in the space lab, and the resulting effect upon the quail feeding behaviour the intake of food was restricted. Lenhardt et al (2001) found slightly increased alkaline phosphatase activity in the microvilli of the enterocytes of the same quails suggesting an adaptation to utilize the restricted feed stuffs more efficiently. We suppose that the insufficient feed intake caused a mobilization of fat reserves in the body and accumulation of lipids in the liver.…”

Section: Discussionmentioning

confidence: 81%

Effect of Short-term Space Flight on Structure of Liver, Lungs, Bone and Bone arrow of Japanese Quail Hatched on Orbital Station Mir

Zibrín¹,

Cigánková²,

Kocisová³

et al. 2005

Acta Vet. Brno

Self Cite

View full text Add to dashboard Cite

The effect of hypergravity of launch, microgravity during short-term space flight, and hypergravity of landing on the structure of liver, lung, bone and bone marrow of 3 Japanese quail, that hatched on board the orbital station Mir was investigated. Chicks were 5 days old at landing. Eleven hours after landing 3 male chicks were killed and samples were collected for a wide variety of biochemical, histological, histochemical, electron microscopic and microbiological investigations. Results of the light microscopic, and transmission electron microscopic study are presented in this paper. Extensive deposits of fat droplets were revealed in almost all hepatocytes of all flight chicks versus ground and laboratory controls. Adipocytes, common in the bone marrow of control animals of the same age, were depleted or absent in bone marrow of flight chicks. There were no substantial effects on the structure/ultrastructure of the lungs, the structure of the bone and its development was not substantially affected. Morphometry of the liver revealed that hepatocytes contained significantly more lipid droplets (nearly 5-fold) than hepatocytes of ground control chicks of the same age hatched at the same time. Morphometric study of lungs revealed no significant difference in number, diameter and total surface of air capillaries between flight and control chicks of the same age.We propose that structural changes in the liver and bone marrow were not caused by direct effect of microgravity per se, but most probably resulted from an insufficient intake of feed due to stress of the microgravity environment and the resulting delay in uptake of lipids from liver. We suppose that these structural changes are the tissue and cellular response to microgravity and they are consistent with literature data known as "space adaptation syndrome". Japanese quails, space flight, effect of microgravity, light and electron microscopyAbility of humans to be sustained in space, whether in orbital space labs and stations or during future long-distance space missions requires supplementing the astronauts' diet. Birds appear to be the most effective converters of plant biomass to animal proteins in an closed system. Among birds, the Japanese quail is considered the most advantageous for spaceflight travel as it has very high rate of reproduction and in relation to its body weight the Japanese quail produces 2.5 times more egg biomass than the domesticated chicken. The orbital station or spacecraft constitute an artificial closed ecosystem operating near 0-G (in weightlessness or microgravity) conditions (Boìa 1993). Accordingly, it is necessary to know in detail the life cycle of Japanese quail, particularly stages sensitive to microgravity or weightlessness, i.e., the production of gametes, fertilization, critical stages of embryogenesis, hatching, early post-incubation development, growth, and maturation. The aim of this study was to investigate the effect of microgravity during brief space flight on ACTA VET. BRNO 2005, 74: 167-174

show abstract

“…In previous studies we observed that the chicks were not able to feed normally during the fi rst days after hatching due to short-term space microgravity. This leads to a signifi cant increase in the AP activity in the duodenal and jejunal enterocytes of Japanese quails on days 4-5 of age (Lenhardt et al 2001). These results indicate an altered functional development of its digestive tract, however, the response of the small intestine to hypodynamy in older chicks is poorly understood.…”

Section: Simulatedmentioning

confidence: 97%

Effect of Long-Term Hypodynamy on Alkaline Phosphatase Activity of Small Intestine in Japanese Quail Chicks

et al. 2007

Self Cite

View full text Add to dashboard Cite

Lenhardt Ľ., V. Cigánková, V. Almášiová, K. Holovská Jr., P. Škrobánek, Š. Mozeš, M. Zibrín: Effect of Long-Term Hypodynamy on Alkaline Phosphatase Activity of Small Intestine in Japanese Quail Chicks. Acta Vet. Brno 2007, 76: 333-338. The functional development of the small intestine was investigated in Japanese quail chicks subjected to simulated microgravity (hypodynamy) on the second day after hatching and reared under these conditions to 63 days of age. On days 5, 7, 14, 21, 28, 35, 42, 56 and 63 the activity of brush-border-bound alkaline phosphatase (AP) in the duodenum and jejunum were determined in experimental animals as well as in control quail chicks housed in a fl oor box during these periods. As compared with control quails the experimental animals displayed a signifi cantly increased enzyme activity until day 42 in the duodenum and day 35 in the jejunum (P < 0.001) whereas in older quails no signifi cant enzymatic differences between these groups was found. However, a decrease in food consumption due to a partial physical constraint cannot be excluded. Moreover, the results suggested that the activity of AP in the control birds did not change substantially during all the periods examined. In contrast, in older hypodynamy quail the AP activity signifi cantly decreased in the duodenum on days 56 and 63 and in the jejunum on days 42, 56 and 63, respectively. These results indicate that a) the enhanced intestinal function in early periods of life may refl ect the higher sensitivity of small intestine to simulated weightlessness, b) the decrease of the AP activity in older animals to the level of controls might be considered as a part of intestinal mechanisms involved in adaptation of quail chicks to long-term hypodynamy, c) differnt activity of AP in the small intestine of Japanese quail may not have resulted only from hypodynamy but also due to decreased food intake.Simulated microgravity, enzymatic activity, duodenum and jejunum, brush-border, growth, Japanese quail

show abstract

Functional Development of the Small Intestine of Japanese Quail Hatched on Mir Orbital Station

Cited by 8 publications

References 24 publications

Reptiles in Space Missions: Results and Perspectives

Reptiles in Space Missions: Results and Perspectives

Effect of Short-term Space Flight on Structure of Liver, Lungs, Bone and Bone arrow of Japanese Quail Hatched on Orbital Station Mir

Effect of Long-Term Hypodynamy on Alkaline Phosphatase Activity of Small Intestine in Japanese Quail Chicks

Contact Info

Product

Resources

About