Functional divergence of GLP genes between G. barbadense and G. hirsutum in response to Verticillium dahliae infection

Jin, Yang; Fan, Liqiang; Zhang, Yihao; Hu, Wei; Han, Xiao; Yan, Qingdi; Yang, Jiaxiang; Li, Fuguang; Yang, Zhaoen

doi:10.1016/j.ygeno.2022.110470

Cited by 4 publications

(4 citation statements)

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“…Similarly, VdSho1 , which encodes a tetraspanin, is required for V. dahliae penetration into cotton plants, and a deletion mutant showed only the slight discoloration of the vascular vessels, concomitant with a significant reduction of pathogenicity [ 23 ]. Consistently, the qRT-PCR results reported to date also show that the genes VdSec22 and VdSyn8, which are related to secretion [ 5 , 27 ], VdMsb and VdMcm1, which are associated with attachment [ 25 , 26 ], and VdCrz1 , which is involved in signal transduction [ 22 ], are all significantly downregulated in the VDAG_07742 deletion mutants, further confirming the involvement of VDAG_07742 in V. dahliae pathogenicity.…”

Section: Discussionsupporting

confidence: 77%

“…Indeed, further experiments showed the defective penetration of a cellulose membrane by the VDAG_07742 knockout mutants and limited fungal growth by a reduced ability of the mutant V. dahliae to penetrate the root cell wall. Previous studies showed that the penetration ability of V. dahliae is closely related to its pathogenicity [ 5 , 45 , 46 ]. Specifically, VdNoxB and VdPls1 are reportedly related to the formation of infection of the nails during V. dahliae penetration [ 24 ] and the deletion of the NADPH oxidase A-encoding gene NoxA , significantly and concomitantly reduced the pathogenicity and the ability of V. dahliae to penetrate cellophane [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Verticillium dahliae , a hemibiotrophic pathogen, is a highly virulent and destructive phytopathogenic fungus that causes Verticillium wilt in more than 600 plant species worldwide, many of them crop species, leading to great economic losses [ 1 , 2 , 3 ]. The infectious cycle of V. dahliae begins with the germination of the microsclerotia to form infective hyphae, which adhere to the host root surface to form hyphopodia that penetrate the host plant root epidermal cells before continuing to invade and block vascular vessels, eventually causing vascular tissue discoloration, leaf chlorosis, and necrosis, and ultimately, plant death [ 4 , 5 ]. The pathogenic mechanism of this fungus is very complex, with the infection process being divided into three stages: (1) in the early stage, spores germinate to form hyphae and adhere to the plant roots; (2) in the intermediate stage, the hyphae successfully invade the root epidermal cells, causing fungal colonization; (3) at the late stage of infection, wilting symptoms appears in the aboveground plant body [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato

Wang

Wen

Zhang

et al. 2023

IJMS

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Verticillium dahliae is a soil-borne pathogenic fungus that causes Verticillium wilt in host plants, a particularly serious problem in potato cultivation. Several pathogenicity-related proteins play important roles in the host infection process, hence, identifying such proteins, especially those with unknown functions, will surely aid in understanding the mechanism responsible for the pathogenesis of the fungus. Here, tandem mass tag (TMT) was used to quantitatively analyze the differentially expressed proteins in V. dahliae during the infection of the susceptible potato cultivar “Favorita”. Potato seedlings were infected with V. dahliae and incubated for 36 h, after which 181 proteins were found to be significantly upregulated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that most of these proteins were involved in early growth and cell wall degradation. The hypothetical, secretory protein with an unknown function, VDAG_07742, was significantly upregulated during infection. The functional analysis with knockout and complementation mutants revealed that the associated gene was not involved in mycelial growth, conidial production, or germination; however, the penetration ability and pathogenicity of VDAG_07742 deletion mutants were significantly reduced. Therefore, our results strongly indicate that VDAG_07742 is essential in the early stage of potato infection by V. dahliae.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato

Wang

Wen

Zhang

et al. 2023

IJMS

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“…A similar observation was noted for 11 GhGLP genes against Verticillium dahliae-infected Gossypium barbadense . Recently JA and phytoalexin-mediated protection was noted for OsGLP2-7 against leaf blast, panicle blast, and bacterial blight …”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. Zea mays contains 26 germin-like protein genes ( ZmGLPs ) located on chromosomes 2, 4, and 10; most of which are functionally unexplored. The present study aimed to characterize all ZmGLPs using the latest computational tools. All of them were studied at a physicochemical, subcellular, structural, and functional level, and their expression was predicted in plant development, against biotic and abiotic stresses using various in silico approaches. Overall, ZmGLPs showed greater similarity in their physicochemical properties, domain architecture, and structure, mostly localized in the cytoplasmic or extracellular regions. Phylogenetically, they have a narrow genetic background with a recent history of gene duplication events on chromosome 4. Functional analysis revealed novel enzymatic activities of phosphoglycolate phosphatase, adenosylhomocysteinase, phosphoglycolate phosphatase-like, osmotin/thaumatin-like, and acetohydroxy acid isomeroreductase largely mediated by disulfide bonding. Expression analysis revealed their crucial role in the root, root tips, crown root, elongation and maturation zones, radicle, and cortex with the highest expression being observed during germination and at the maturity levels. Further, ZmGLPs showed strong expression against biotic ( Aspergillus flavus , Colletotrichum graminicola , Cercospora zeina , Fusarium verticillioides , and Fusarium virguliforme ) while limited expression was noted against abiotic stresses. Concisely, our results provide a platform for additional functional exploration of the ZmGLP genes against various environmental stresses.

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Role of Germin-Like Proteins (GLPs) in Biotic and Abiotic Stress Responses in Major Crops: A Review on Plant Defense Mechanisms and Stress Tolerance

Govindan,

K R,

Alphonse

et al. 2024

Plant Mol Biol Rep

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Functional divergence of GLP genes between G. barbadense and G. hirsutum in response to Verticillium dahliae infection

Cited by 4 publications

References 44 publications

Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato

Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Role of Germin-Like Proteins (GLPs) in Biotic and Abiotic Stress Responses in Major Crops: A Review on Plant Defense Mechanisms and Stress Tolerance

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