Functional Diversification of Kaurene Synthase-Like Genes in <i>Isodon rubescens</i>

Jin, Baolong; Cui, Guanghong; Guo, Juan; Tang, Jian; Duan, Lixin; Lin, Huixin; Shen, Ye; Chen, Tong; Zhang, Huabei; Huang, Luqi

doi:10.1104/pp.17.00202

Cited by 47 publications

(59 citation statements)

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“…6 ). IeCPS2 and IrCPS4 have been determined to be involved in pharmacologically active Isodon diterpenoids biosynthesis [ 18 , 19 ]. Therefore, the CPS candidate genes, especially IaCPS1 (DN64742_c2_g3), may have important roles in Wangzaozins biosynthesis.…”

Section: Resultsmentioning

confidence: 99%

“…IaKSL2 and IaKSL4 were closely related to IrKSL5 and IrKSL4, respectively. IrKSL2 and IrKSL5 have been shown to react with ent- CPP [ 19 ]. The results indicate that KSL genes (IaKSL2 and IaKSL4) having a high or predominant expression, may be a cause of accumulation of Wangzaozins in I. amethystoides leaves.…”

Section: Resultsmentioning

confidence: 99%

“…Wangzaozins, belonging to ent- kaurane-type tetracyclic diterpenoids [ 9 , 10 ], share the same requisite skeletal structure with gibberellin, as well as oridonin. Oridonin has been reported to have the same precursor of ent- kaurene with gibberellin [ 19 , 27 ]. Therefore, ent- kaurene is thought to be the common substrate for biosynthesis of gibberellin and Wangzaozins.…”

Section: Resultsmentioning

confidence: 99%

“…Phylogenetic analysis revealed that it shares a high amino acid identity with I. rubescens IrCPS3. IrCPS3 was expressed five-fold higher in stem than root and leaf [ 19 ]. Other CPS transcripts share a high homology with known CPS genes from other plant species.…”

Section: Discussionmentioning

confidence: 99%

“…For Isodon species, Li et al [ 18 ] reported the role of two IeCPS genes in ent- copalyl diphosphate synthesis in I. eriocalyx via homologous cloning. Very recently, the diterpene synthases (diTPSs) including CPS and KSL have been identified in I. rubescens [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis

et al. 2018

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BackgroundIsodon amethystoides (Ben-th) Cy Wu et Hsuan is an important traditional medicinal plant endowed with pharmacological properties effective in the treatment of various diseases, including pulmonary tuberculosis. The tetracyclic diterpenoids, Wangzaozins (Wangzaozin A, glaucocalyxin A, glaucocalyxin B), are the major bioactive compounds of I. amethystoides. However, the molecular information about the biosynthesis of these compounds still remains unclear.ResultsAn examination of the accumulated levels of Wangzaozins in I. amethystoides revealed considerable variations in the root, stem, and leaf tissues of this plant, indicating possible differences in metabolite biosynthesis and accumulation among various tissues. To better elucidate the tetracyclic diterpenoid biosynthesis pathway, we generated transcriptome sequences from the root, stem, and leaf tissues, and performed de novo sequence assembly, yielding 230,974 transcripts and 114,488 unigenes, with average N50 lengths of 1914 and 1241 bp, respectively. Putative functions could be assigned to 73,693 transcripts (31.9%) based on BLAST searches against annotation databases, including GO, KEGG, Swiss-Prot, NR, and Pfam. Moreover, the candidate genes involving in the diterpenoid biosynthesis, such as CPS, KSL, were also analyzed. The expression profiles of eight transcripts, involving the tetracyclic diterpenoid biosynthesis, were validated in different I. amethystoides tissues by qRT-PCR, unraveling the gene expression profile of the pathway. The differential expressions of ISPD, ISPF and ISPH (MEP pathway), and IaCPS and IaKSL (diterpenoid pathway) candidate genes in leaves and roots, may contribute to the high accumulation of Wangzaozins in I. amethystoides leaves.ConclusionThe genomic dataset and analyses reported here lay the foundations for further research on this important medicinal plant.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1505-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis

et al. 2018

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Recent progress and new perspectives for diterpenoid biosynthesis in medicinal plants

Liu

Tian

et al. 2021

Medicinal Research Reviews

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Diterpenoids, including more than 18,000 compounds, represent an important class of metabolites that encompass both phytohormones and some industrially relevant compounds. These molecules with complex, diverse structures and physiological activities, have high value in the pharmaceutical industry. Most medicinal diterpenoids are extracted from plants. Major advances in understanding the biosynthetic pathways of these active compounds are providing unprecedented opportunities for the industrial production of diterpenoids by metabolic engineering and synthetic biology. Here, we summarize recent developments in the field of diterpenoid biosynthesis from medicinal herbs. An overview of the pathways and known biosynthetic enzymes is presented. In particular, we look at the main findings from the past decade and review recent progress in the biosynthesis of different groups of ringed compounds. We also discuss diterpenoid production using synthetic biology and metabolic engineering strategies, and draw on new technologies and discoveries to bring together many components into a useful framework for diterpenoid production.

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Identification and functional characterization of three cytochrome P450 genes for the abietane diterpenoid biosynthesis in Isodon lophanthoides

Peng

et al. 2023

Planta

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Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens

Cited by 47 publications

References 44 publications

Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis

Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis

Recent progress and new perspectives for diterpenoid biosynthesis in medicinal plants

Identification and functional characterization of three cytochrome P450 genes for the abietane diterpenoid biosynthesis in Isodon lophanthoides

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