Functional DNA nanomaterials for sensing and imaging in living cells

Torabi, Seyed Fakhreddin; Lu, Yi

doi:10.1016/j.copbio.2013.12.011

Cited by 63 publications

(35 citation statements)

References 62 publications

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“…Aptamers bound to gold nanoparticles are hybridized with fluorescent DNA strands that are quenched by proximity to the nanoparticle. Binding of ATP to the aptamers releases the fluorescent strands, and the increase in fluorescence can be used to quantitate ATP in live cells (Zheng et al , 2009; Torabi and Lu, 2014). One drawback of these biosensors is that the aptamers have been engineered for adenine selectivity, which can make it challenging to distinguish between adenine derivatives (Ozalp et al , 2010).…”

Section: Methods For Detection and Imaging Atpmentioning

confidence: 99%

Imaging Adenosine Triphosphate (ATP)

Rajendran

Dane

Conley

et al. 2016

The Biological Bulletin

110

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Adenosine triphosphate (ATP) is a universal mediator of metabolism and signaling across unicellular and multicellular species. There is a fundamental interdependence between the dynamics of ATP and the physiology that occurs inside and outside the cell. Characterizing and understanding ATP dynamics provides valuable mechanistic insight into processes that range from neurotransmission to the chemotaxis of immune cells. Therefore, we require the methodology to interrogate both temporal and spatial components of ATP dynamics from the subcellular to organismal levels in live specimens. Over the last several decades, a number of molecular probes that are specific for ATP have been developed. These probes have been combined with imaging approaches, particularly optical microscopy, to enable qualitative and quantitative detection of this critical molecule. In this review, we survey current examples of technologies that are available to visualize ATP in living cells and identify areas where new tools and approaches are needed to expand our capabilities.

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Section: Methods For Detection and Imaging Atpmentioning

confidence: 99%

Imaging Adenosine Triphosphate (ATP)

Rajendran

Dane

Conley

et al. 2016

The Biological Bulletin

110

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“…Isolated with an in vitro process called Systematic Evolution of Ligand by EXponential enrichment, or SELEX (Ellington and Szostak, 1990, Tuerk and Gold, 1990, Beaudry and Joyce, 1992, Ellington and Szostak, 1992, Breaker et al, 1994), DNA/RNA aptamers and DNAzymes/ribozymes can be isolated to bind to a wide range of targets with high affinity and specificity, including metal ions, small molecules, protein and even virus or whole cell, making them one of the most versatile recognition elements (Lu and Liu, 2006, Navani and Li, 2006, Shangguan et al, 2006, Mok and Li, 2008, Li and Lu, 2009, Liu et al, 2009, Wu et al, 2010, Sai Lau and Li, 2011, Zhang et al, 2011, Ali et al, 2012, Torabi and Lu, 2014, Xiang and Lu, 2014, Shen et al, 2015). In addition to target-binding capability, DNAzymes can catalyze chemical reactions, the most common being hydrolysis of a phosphodiester bond (Breaker et al, 1994, Carmi et al, 1996, Santoro and Joyce, 1997, Carmi et al, 1998, Santoro and Joyce, 1998, Li et al, 2000, Carmi and Breaker, 2001, Brown et al, 2003, Liu et al, 2007, Brown et al, 2009, Torabi et al, 2015).…”

Section: Design Of the Bgm-based Biosensors For Ivds Of Non-glucosmentioning

confidence: 99%

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Lan

Zhang

2016

Biotechnology Advances

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Recent advances in mobile network and smartphones have provided an enormous opportunity for transforming in vitro diagnostics (IVD) from central labs to home or other points of care (POC). A major challenge to achieving the goal is a long time and high costs associated with developing POC IVD devices in mobile Health (mHealth). Instead of developing a new POC device for every new IVD target, we and others are taking advantage of decades of research, development, engineering and continuous improvement of the blood glucose meter (BGM), including those already integrated with smartphones, and transforming the BGM into a general healthcare meter for POC IVDs of a wide range of biomarkers, therapeutic drugs and other analytical targets. In this review, we summarize methods to transduce and amplify selective binding of targets by antibodies, DNA/RNA aptamers, DNAzyme/ribozymes and protein enzymes into signals such as glucose or NADH that can be measured by commercially available BGM, making it possible to adapt many clinical assays performed in central labs, such as immunoassays, aptamer/DNAzyme assays, molecular diagnostic assays, and enzymatic activity assays onto BGM platform for quantification of non-glucose targets for a wide variety of IVDs in mHealth.

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“…72,73 A number of these strategies have been discussed in more depth in recent reviews and will not be covered in detail in this review. 3,4,74,75 …”

Section: Introductionmentioning

confidence: 99%