Functional efficacy analysis ofAngelica gigasNakai on chicken myoblast cells through cell‐based in vitro assay

Abstract

The value‐added products in livestock industry is one of the key issues in order to maximize the revenue and to create a new business model. Numerous studies have suggested application of herbal plants as feed additives to increase health, productivity, and/or high‐quality product in livestock. In this study, the first experiment was designed to develop in vitro evaluation system by using primary chicken myoblast (pCM) cells isolated from pectoralis major of 10‐day‐old male embryos. Subsequently, to evaluate effects of Korean Danggui Angelica gigas Nakai (AGN), we optimized the concentration of AGN root extract for treatment of primary pCM cells. After the treatment of AGN root extract, we compared proliferation and differentiation capacity, and also examined the gene expression. In the second experiment, the next generation sequencing analysis was performed to compare the different patterns of the global gene expression in pCM cells treated with AGN extract. Three up‐regulated (pancreas beta cells, fatty acid metabolism and glycolysis) and one down‐regulated (adipogenesis) gene sets were characterized suggesting that the AGN extract affected the metabolic pathways for the utilization of fat and glucose in chicken muscle cells. Furthermore, we validated the expression patterns of the up‐regulated genes (GCLC, PTPN6, ISL1, SLC25A13, TGFBI, and YWHAH) in the AGN‐treated pCM cells by quantitative RT‐PCR. These results demonstrated that the treatment of AGN extract decreased proliferation and differentiation of pCM cells, and affected the metabolic pathways of glucose and fatty acids. Moreover, AGN extract derived from byproducts such as stem and leaf also showed the reduced proliferation patterns on AGN‐treated pCM cells. Taken together, pCM cell‐based in vitro assay system could be primarily and efficiently applied for evaluating the biofunctional efficacy of various feed additive candidates.

Effects of Dietary Inclusion of Angelica gigas Nakai root extract on the Growth Performance, Hematological and Serum Biochemical Parameters in Broilers

“…Additionally, Zhou et al (2015) and Niu et al (2019) reported that dietary supplementation of broilers with fermented Ginkgo biloba rations and fermented Ginkgo biloba leaves resulted in significant decreases in serum levels of triglycerides and cholesterol. Lee et al (2019) reported that AGN as a lipolytic supplement showed the down-regulation of proliferation and differentiation in chicken myoblast cells, and affected the metabolic pathways of glycolysis and fatty acids by suppressing fat accumulation followed by amelioration of hepatic steatosis and hyperlipidemia and activating glycolysis.…”