Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer

The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer

“…From an overview of all these studies, it has become apparent that there are specific tumour suppressor genes that function with particular tumour types ). Excitingly, potential tumour suppressor genes for ovarian cancer have been found on chromosome 22 (22q11-q12) (Kruzelock et al 2000), chromosome 3 (Rimessi et al 1994), chromosome 6 (6q27) (Acquati et al 2001) and chromosome 11 (Cao et al 2001), the latter study has led to the proposition of THY1 being a tumour suppressor gene for ovarian cancer (Abeysinghe et al 2003(Abeysinghe et al , 2004. Further, genes contained on chromosome 6 (Negrini et al 1994) and the short arm of chromosome 8 have been shown, by MMCT studies, to be important in breast cancer (Wilson et al 2003;Seitz et al 2005) and colorectal cancer (Flanagan et al 2004).…”

Cytogenetics and molecular genetics of ovarian cancer

“…Similarly, transfer of chromosome 22 into the ovarian carcinoma cell line SKOV‐3 leads to a complete abrogation of anchorage‐independent cell growth and a dramatic reduction of in vitro doubling times and tumorigenicity in nude mice. Additionally, it is evident by microsatellite marker polymorphism analysis that a tumor growth suppressor gene is located between markers D22S301 and D22S304 in the region of 22q11‐q12 [Kruzelock et al, 2000]. The functional roles of chromosomes 11 and 17 in the carcinogenesis of ovarian carcinoma have not been thoroughly investigated, though chromosomes 11 and 17 have been implicated in many types of human neoplasia.…”