Functional expression and characterization of Echinococcus granulosus thioredoxin peroxidase suggests a role in protection against oxidative damage

Li, Jun; Zhang, Wenbao; Loukas, Alex; Lin, Ren-Yong; Itō, Akira; Zhang, Lihua; Jones, Malcolm K.; McManus, Donald P.

doi:10.1016/j.gene.2003.10.027

Cited by 75 publications

(42 citation statements)

References 29 publications

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“…Thioredoxin peroxidase (TPx) functions as an antioxidant to remove H 2 O 2 and O 2 . ( using thioredoxin as the electron donor (Li et al 2004). As expected, our results showed increased TPx activity and expression levels under drought treatment, which might delay droughtinduced damage by limiting the activity of ROS (He et al 2007).…”

Section: Proteins Associated With the Stress Response To Drought Stresssupporting

confidence: 82%

Proteome response of wild wheat relativeKengyilia thoroldianato drought stress

Yang

Ma³

et al. 2015

Can. J. Plant Sci.

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. 2015. Proteome response of wild wheat relative Kengyilia thoroldiana to drought stress. Can. J. Plant Sci. 95: 237Á249. Wild relatives of crops provide plant breeders with a broad pool of potentially useful genetic sources. The genus Kengyilia, being a member of the tribe Triticeae, is related to wheat, barley, and other cereals and forage grasses. We studied proteomic changes in K. thoroldiana seedlings in response to drought stress after withholding water for 0, 3, 6, 9 and 15 d. To determine the proteomic changes that occurred in leaves of K. thoroldiana under drought stress, two-dimensional gel electrophoresis (2-DE) and mass spectrometry were performed to identify protein expression changes. Seventy proteins showing reproducible and significant expression changes were identified. Among them, 28 proteins were up-regulated, whereas seven proteins were down-regulated. Based on databaseannotated functions, these 70 proteins were categorized as energy metabolism, stress response, antioxidative enzyme, transcript and signal transduction, predicted proteins, and chloroplast-related proteins. Cluster analysis further showed that the up-regulated proteins were mainly stress response proteins and antioxidative enzymes. These results suggest that K. thoroldiana may resist drought stress by increasing the expression of stress response proteins and by producing antioxidative enzymes to remove reactive oxygen species. We conclude that the K. thoroldiana drought stress response mechanism could represent a useful genetic resource for related studies in wheat.Key words: Proteome, wild wheat relative, Kengyilia thoroldiana, drought stress Yang, S., Li, X., Ma, Y., Sun, X., Yang, Y. et Yang, Y. 2015. Re´action du prote´ome du parent sauvage du ble´Kengyilia thoroldiana au stress duˆa`la se´cheresse. Can. J. Plant Sci. 95: 237Á249. Les parents sauvages des plantes cultive´es offrent aux phytoge´ne´ticiens un vaste re´servoir de ge`nes potentiellement utiles. Le genre Kengyilia, qui fait partie de la tribu des Tritice´es, est apparente´au ble´, a`l'orge ainsi qu'a`d'autres ce´re´ales et gramine´es fourrage`res. Les auteurs ont e´tudie´les changements prote´omiques subis par les plantules de K. thoroldiana a`la suite de la se´cheresse apre`s les avoir prive´s d'eau pendant 0, 3, 6, 9 ou 15 jours. Pour ve´rifier les changements prote´omiques survenus dans les feuilles de K. thoroldiana a`la suite du stress duˆa`la se´cheresse, ils ont identifie´les modifications subies par les prote´ines au moyen de l'e´lectrophore`se bidirectionnelle sur ge´lose et de la spectrome´trie de masse. Soixante-dix prote´ines exprimant des changements importants et reproductibles ont ainsi e´te´identifie´es. Parmi elles, 28 e´taient re´gule´es en amont et sept en aval. D'apre`s leurs fonctions, annote´es dans les bases de donne´es, ces 70 prote´ines ont e´te´cate´gorise´es parmi celles qui touchent au me´tabolisme de l'e´nergie, a`la re´action au stress, aux enzymes antioxydants et a`la transduction des transcriptions et des signaux ainsi que

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Section: Proteins Associated With the Stress Response To Drought Stresssupporting

confidence: 82%

Proteome response of wild wheat relativeKengyilia thoroldianato drought stress

Yang

Ma³

et al. 2015

Can. J. Plant Sci.

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“…However, the thioredoxin system participates in the defense against oxidative stress via the reduction of peroxiredoxins. These enzymes have been reported in cestodes (Li et al 2004;Vaca-Paniagua et al 2009). …”

Section: Discussionmentioning

confidence: 68%

In vitro killing action of auranofin on Taenia crassiceps metacestode (cysticerci) and inactivation of thioredoxin–glutathione reductase (TGR)

et al. 2010

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Control of cellular redox homeostasis is a central issue for all living organisms. Glutathione and thioredoxin enzymatic redox systems are the usual mean used to achieve such a control. However, parasitic platyhelminths studied to date possess a nicotinamide adenine dinucleotide phosphate-dependent thioredoxin-glutathione reductase (TGR) as the sole redox control system. Thus, TGR is considered as a potential therapeutic target of parasitic platyhelminths, and based on this assumption, the gold compound auranofin is a potent inhibitor of TGR. The aim of this research was to investigate the effect of auranofin on metacestode (cysticerci) of Taenia crassiceps in culture. Accordingly, the time course for viability and respiration of cysticerci in culture was evaluated in the presence of this compound. After 4 h at 10 microM auranofin, 90% of cysticerci were alive, but respiration activity had declined by 50%. After 12 h, neither survivors nor respiration was detected; a LD(50) for auranofin of 3.8 microM was calculated. Interestingly, crude extracts of cysticerci pretreated with 3 microM auranofin nearly nil TGR activity (IC(50) = 0.6 microM). Zymography for TGR in polyacrylamide gel electrophoresis was conducted because the previously mentioned extracts clearly showed a dose-response inactivation of TGR toward auranofin. The killing of cysticerci by this gold compound is most likely related with TGR inactivation. Therefore, further research on the suitability of auranofin as a therapeutic tool in the treatment of cysticercosis in animals and humans is sustained.

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“…In vivo oxidative stress bioassay was performed as described previously by Li et al 2004. Briefly, rBmTCTP-transformed bacteria [BL21(DE3)] were grown until the OD reading of the culture reached 0.6.…”

Section: H 2 O 2 Tolerance Bioassaymentioning

confidence: 99%

Translationally controlled tumor protein of Brugia malayi functions as an antioxidant protein

Munirathinam

Kalyanasundaram

2007

Parasitol Res

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Translationally controlled tumor protein (TCTP) is one of the most abundantly expressed proteins in the filarial parasites as well as in the other organisms. Several functions have been suggested for TCTP family of proteins ranging from calcium binding to histamine release function. However, its physiological function is still a mystery. Previous studies showed that the expression of TCTP is increased several-fold during oxidative stress. In the present work, we report the putative antioxidant function of Brugia malayi TCTP (BmTCTP). When tested in vitro, rBmTCTP could be reduced by a variety of reducing agents including thioredoxin. Such reduced form of rBmTCTP was able to protect DNA from oxidative damage, suggesting that BmTCTP may have an antioxidant function in the parasite. Sequence analysis of filarial TCTPs revealed that there are three cysteine amino acids located in the central portion of the protein. Subsequent targeted residue modification studies showed that these cysteine residues in rBmTCTP are critical for its antioxidant function. To determine the significance of this finding, rBmTCTP was overexpressed in vivo in Escherichia coli and subjected to oxidative stress. These studies showed that rBmTCTP significantly protected cells form oxidative damage. Taken together, these findings suggest that BmTCTP might be functioning as a non-classical antioxidant protein in the filarial parasites.

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Functional expression and characterization of Echinococcus granulosus thioredoxin peroxidase suggests a role in protection against oxidative damage

Cited by 75 publications

References 29 publications

Proteome response of wild wheat relativeKengyilia thoroldianato drought stress

Proteome response of wild wheat relativeKengyilia thoroldianato drought stress

In vitro killing action of auranofin on Taenia crassiceps metacestode (cysticerci) and inactivation of thioredoxin–glutathione reductase (TGR)

Translationally controlled tumor protein of Brugia malayi functions as an antioxidant protein

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