1997
DOI: 10.1046/j.1471-4159.1997.69041491.x
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Functional Expression of a GLT‐1 Type Na+‐Dependent Glutamate Transporter in Rat Pinealocytes

Abstract: Pinealocytes, the neuroendocrine cells that produce melatonin, accumulate glutamate in microvesides through a specific vesicular transporter energetically coupled with vacuolar-type proton ATPase. The glutamate is secreted into the extracellular space through microvesicle-mediated exocytosis and then stimulates neighboring pinealocytes, resulting in inhibition of norepinephrine-dependent melatonin synthesis. In this study, we identified and characterized the plasma membranetype glutamate transporter in rat pin… Show more

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Cited by 36 publications
(27 citation statements)
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“…We used the following cell markers to classify DNPI-positive cells: synaptophysin for pinealocytes (30,31), glial fibrillary acidic protein for astrocytes (32), OX42 for microglia (33), and vimentin for interstitial cells (34). The antibodies against these marker proteins immunostained the corresponding populations of pineal cells with a similar morphology as reported previously (33,35) (Fig. 2D).…”
Section: Fig 2 Expression and Localization Of Dnpi In Pinealocytessupporting
confidence: 67%
“…We used the following cell markers to classify DNPI-positive cells: synaptophysin for pinealocytes (30,31), glial fibrillary acidic protein for astrocytes (32), OX42 for microglia (33), and vimentin for interstitial cells (34). The antibodies against these marker proteins immunostained the corresponding populations of pineal cells with a similar morphology as reported previously (33,35) (Fig. 2D).…”
Section: Fig 2 Expression and Localization Of Dnpi In Pinealocytessupporting
confidence: 67%
“…Cultured rat pinealocytes can take up exogenous -Asp intensively (Takigawa et al 1998). The -Glu transporter, which has been identified in the rat pineal gland (Yamada et al 1997) may be responsible for this uptake since this transporter has affinity for -Asp in addition to -Glu and -Asp (Kanai & Hediger 1992, Pines et al 1992. The capacity of the testis to concentrate -Asp suggests the presence of -Glu receptors, whose expression could vary in correlation with gonadal activity.…”
Section: Discussionmentioning
confidence: 99%
“…The transport of Glu in MV and its effect on pineal metabolic activity has been well studied in several mammals (Govitrapong and Ebadi, 1988;McNulty et al, 1992;Kus et al, 1993Kus et al, , 1994Redecker and Veh, 1994;van Wyk and Daya, 1994;Moriyama and Yamamoto, 1995a,b;Yamada et al, 1996aYamada et al, ,b, 1997bYamada et al, , 1998a. Glu is taken up into both pinealocytes (Yamada et al, 1997b) and interstitial cells (Redecker and Pabst, 2000) mainly via a type 1 Na ϩ -dependent Glu transporter and then stored in MV via the synaptic vesicle protein of type 2 (SV2B, Hayashi et al, 1998). Following cell depolarization, Glu is released by exocytosis via Ca 2ϩ -dependent mechanisms.…”
Section: B Other Nonadrenergic Nonpeptidergic Transmitters Of the Pmentioning
confidence: 99%