Functional expression of a stably transfected parathyroid hormone/parathyroid hormone related protein receptor complementary DNA in CHO cells

Kaufmann, M.; Muff, Roman; Born, Walter; Fischer, Jan A.

doi:10.1016/0303-7207(94)90047-7

Cited by 26 publications

(13 citation statements)

References 14 publications

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“…[Ca2+Ii of small cerebellar cells attached to the glass coverslip within the bottom of a plastic Petri dish was determined after loading the cells with 1 pM fura-2 AM (Calbiochem) in incubation buffer (136 mM NaCl, 5.4 mM KC1, 1.3 mM Na,HPO,, 1 mM MgSO,, 1 mM CaCl,, 1 mg/ml glucose and 10 mM HEPES, pH 7.4) for 45 min at 37°C (Kaufmann et al, 1994). After loading, the cells were washed and incubated for 45 min at 37°C in 150 pl incubation buffer.…”

Section: Ii)mentioning

confidence: 99%

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CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the α6/β1 integrin and the neural adhesion molecule L1

Schmidt

Künemund

Wintergerst

et al. 1996

J of Neuroscience Research

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We describe here a novel monoclonal antibody (mab H6) which recognizes CD9, an integral cell surface constituent previously described in cells of the hematopoietic lineage and involved in the aggregation of platelets. Mab H6 was raised against membranes of immature mouse astrocytes and reacted with a protein of 25-27 kD in detergent extracts of adult mouse brain membranes. Sequence analysis of the N-terminal amino acids revealed an identity of 96% with CD9 from mouse kidney. CD9 was localized in the central and peripheral mouse nervous systems: in the spinal cord of 11-day-old mouse embryos, CD9 was strongly expressed in the floor and roof plates. In the adult mouse sciatic nerve, myelin sheaths were highly CD9-immunoreactive. Mab H6 reacted with the cell surfaces of both glial cells and neurons in culture and inhibited migration of neuronal cell bodies, neurite fasciculation and outgrowth of astrocytic processes from cerebellar microexplants. Neurite outgrowth from isolated small cerebellar neurons was increased in the presence of mab H6 on substrate-coated laminin, but not on substrate-coated poly-L-lysine. Addition of mab H6 elicited an increase in intracellular Ca2+ concentration in these cells on substrate-coated laminin. Immunoprecipitates of CD9 from cultured mouse neuroblastoma N2A cells contained the alpha 6/beta 1 integrin. Moreover, preparations of CD9 immunoaffinity-purified from adult mouse brain using a mab H6 column contained the neural adhesion molecule L1, but not other neural adhesion molecules. CD9 bound to L1, but not to NCAM or MAG. Both the alpha 6/beta 1 integrin and L1 could be induced to coredistribute with CD9 on the surface of cultured neuroblastoma N2A cells. The combined observations suggest that CD9 can associate with L1 and alpha 6/beta 1 integrin to influence neural cell interactions in vitro.

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Section: Ii)mentioning

confidence: 99%

“…Mabs H6 and H28 were added in 50 p1 incubation buffer at a final concentration of 250 pg/ml). [Ca2+Ii was measured at 37°C by dual excitation microfluometry on an inverted microscope (epifluorescence mode, objective NIKON F50; Kaufmann et al, 1994). Nine independent experiments were carried out.…”

Section: Ii)mentioning

confidence: 99%

CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the α6/β1 integrin and the neural adhesion molecule L1

Schmidt

Künemund

Wintergerst

et al. 1996

J of Neuroscience Research

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“…19,34,35 Apical receptors may be preferentially coupled to the cAMP-independent signaling pathway, while basolateral receptor activation initiates both cAMP-dependent and -independent effects. 36,37 Megalin, a multifunctional clearance receptor expressed on the apical surface of proximal tubular cells, has been shown to contribute to catabolism of PTH and it may antagonize PTH/PTHrP receptor activity.…”

Section: The Pth/pthrp Receptor Systemmentioning

confidence: 99%

Genetic Disorders Caused by Mutations in the PTH/PTHrP Receptor and Downstream Effector Molecules

Silve¹,

Jüppner²

2015

The Parathyroids

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“…Detection limits of the assay, equal to a s 3-fold stimulation of cyclic AMP accumulation above basal levels, ranged from 5-15 fmol hIAPP and 25-75 fmol mlAPP. Samples were incubated with T47D cells in 24-well plates (23) and cyclic AMP in cell extracts was determined as previously described (25).…”

Section: In Vitro Bioassaymentioning

confidence: 99%

Biologically active human islet amyloid polypeptide/amylin in transgenic mice

Hulst

Born²,

Muff³

et al. 1997

European Journal of Endocrinology

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Functional expression of a stably transfected parathyroid hormone/parathyroid hormone related protein receptor complementary DNA in CHO cells

Cited by 26 publications

References 14 publications

CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the α6/β1 integrin and the neural adhesion molecule L1

CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the α6/β1 integrin and the neural adhesion molecule L1

Genetic Disorders Caused by Mutations in the PTH/PTHrP Receptor and Downstream Effector Molecules

Biologically active human islet amyloid polypeptide/amylin in transgenic mice

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