Functional Expression of Miraculin, a Taste-modifying Protein, in Transgenic Miyagawa Wase Satsuma Mandarin (Citrus unshiu Marc.)

Bachchu, Mohammad Adnan Al; Jin, Shuilin; Park, Jeongwon; Boo, Kyung-Hwan; Sun, Hyeon-Jin; Kim, Yong-Woo; Lee, Hyo-Yeon; Riu, Key Zung; Kim, Jae-Hoon

doi:10.3839/jksabc.2011.003

Cited by 7 publications

(1 citation statement)

References 13 publications

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“…최근 들어 miraculin 기능을 산업용 또는 재조합 miraculin 생산을 위해 대장균 (Kurihara 1992), 효모 (Kurihara 1997) 및 Aspergillus oryzae (Ito et al 2007)와 같은 다양한 숙주세포를 이용하여 실험한 결과 미라클 프루트 열매로부터 얻은 활성보다 훨씬 낮은 결과를 초래하였다. 반면에 식물 발현시스템을 통한 몇몇 실험에서 는 재조합 miraculin 활성과 천연 miraculin활성과 거의 비슷 한 결과를 얻었다 (Sun et al 2006, Sun et al 2007, Al Bachchu et al 2011, Park et al 2020. 이런 결과는 N-glycosylation 뿐만 아 니라 miraculin에서 β1,2-xylose (Xyl)과 α1,3-fucose (Fuc)잔기 들을 가진 식물 특이적 N-glycans이 단백질 접힘(folding), 안 정성 및 그에 따른 활성 등에 매우 중요하다고 하였다 (Kajiura et al 2018…”

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Production of miraculin protein in suspension cell lines of transgenic rice usingAgrobacterium

Kyoung

Park

et al. 2020

J Plant Biotechnol

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To produce the miraculin protein in suspension cultures, rice (Oryza sativa L.) was transformed with Agrobacterium tumefacience EHA105 containing the miraculin AB512278 gene. The cell suspension cultures were established using cell lines selected from transgenic rice callus. The integration of the miraculin gene into the rice chromosome was confirmed using genomic PCR analysis. In addition, RT-PCR analysis indicated that the miraculin gene is expressed in the selected suspension cell lines. Thus, the recombinant miraculin was expressed in the transgenic suspension cell line, HK-2. Therefore, we have successfully developed a HK-2 line that produces miraculin. These results demonstrate that transformed cell suspension cultures can be used to produce a taste-modifying protein such as miraculin.

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Production of miraculin protein in suspension cell lines of transgenic rice usingAgrobacterium

Kyoung

Park

et al. 2020

J Plant Biotechnol

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Citrus Transformation Using Juvenile Tissue Explants

Orbović

Grosser

2014

Methods in Molecular Biology

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The most frequently used method for production of citrus transgenic plants is via Agrobacterium-mediated transformation of tissues found on explants obtained from juvenile seedlings. Within the last decade and especially within the last 5-6 years, this robust method was employed to produce thousands of transgenic plants. With the newly applied screening methods that allow easier and faster detection of transgenic shoots, estimates of transformation rate for some cultivars have gone up making this approach even more attractive. Although adjustments have to be made regarding the (varietal) source of the starting material and Agrobacterium strain used in each experiment preformed, the major steps of this procedure have not changed significantly if at all. Transgenic citrus plants produced this way belong to cultivars of rootstocks, sweet oranges, grapefruits, mandarins, limes, and lemons.

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Selection of transgenic citrus plants based on glyphosate tolerance conferred by a citrus 5-enolpyruvylshikimate-3-phosphate synthase variant

et al. 2021

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Functional Expression of Miraculin, a Taste-modifying Protein, in Transgenic Miyagawa Wase Satsuma Mandarin (Citrus unshiu Marc.)

Cited by 7 publications

References 13 publications

Production of miraculin protein in suspension cell lines of transgenic rice usingAgrobacterium

Production of miraculin protein in suspension cell lines of transgenic rice usingAgrobacterium

Citrus Transformation Using Juvenile Tissue Explants

Selection of transgenic citrus plants based on glyphosate tolerance conferred by a citrus 5-enolpyruvylshikimate-3-phosphate synthase variant

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