Functional Features of TREHALOSE-6-PHOSPHATE SYNTHASE1, an Essential Enzyme in Arabidopsis[OPEN]

Fichtner, Franziska; Olas, Justyna Jadwiga; Feil, Regina; Watanabe, Mutsumi; Krause, Ursula; Hoefgen, Rainer; Stitt, Mark; Lunn, John E.

doi:10.1105/tpc.19.00837

Cited by 89 publications

(90 citation statements)

References 74 publications

(141 reference statements)

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“…In arabidopsis leaves, the leaf vasculature is a major site of TPS1 expression, and by implication Tre6P synthesis and signalling (Fig. 1; Fichtner et al ., 2020). Arabidopsis is an apoplastic phloem loading species, in which sucrose is released from phloem parenchyma cells into the apoplast via SUCROSE WILL EVENTUALLY BE EXPORTED type sucrose effluxers (SWEET11 and SWEET12) and then actively taken up into the companion cell‐sieve element complex by the SUT1/SUC2 sucrose‐H + symporter (Lalonde et al ., 2003; Baker et al ., 2012; Chen et al ., 2012; Eom et al ., 2015; Zakhartsev et al ., 2016).…”

Section: Resultsmentioning

confidence: 99%

“…TPS1 localization andtrehalose 6‐phosphate (Tre6P) synthesis during bud development. (a) TPS1 constructs are derived from the arabidopsis TPS1 (At1g78580) genomic locus, including the native promoter ( pAtTPS1 ) and terminator ( tAtTPS1 ) regions (Fichtner et al ., 2020). TPS1 fusion proteins tagged with the GREEN FLUORESCENT PROTEIN (GFP; green box) or the β‐GLUCURONIDASE (GUS; blue box) were used to analyse the TPS1 expression pattern.…”

Section: Methodsmentioning

confidence: 99%

“…1a). Expression of the TPS1 fusion proteins was under the control of the endogenous TPS1 promoter and other potential regulatory elements from the TPS1 genomic locus, and the complemented lines showed normal embryonic and post-embryonic growth (Fichtner et al, 2020;Fig. 1a).…”

Section: Localization and Function Of Tps1 In Arabidopsis Axillary Budsmentioning

confidence: 99%

“…TPS1 localization and Tre6P synthesis during bud development. (a) TPS1 constructs are derived from the arabidopsis TPS1 (At1g78580) genomic locus, including the native promoter (pTPS1) and terminator (tTPS1) regions(Fichtner et al, 2020).TPS1 fusion proteins tagged with the GREEN FLUORESCENT PROTEIN (GFP; green box) or the β-GLUCURONIDASE (GUS; blue box) were used to analyze the TPS1 expression pattern. A construct encoding the full-length TPS1 protein was used as a control together with a construct expressing only the heterologous TPS from Escherichia coli (OtsA) under the control of the TPS1 gene regulatory elements (pTPS1 and tTPS1).…”

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Regulation of shoot branching in arabidopsis by trehalose 6‐phosphate

et al. 2020

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Trehalose 6-phosphate (Tre6P) is a sucrose signalling metabolite that has been implicated in regulation of shoot branching, but its precise role is not understood. We expressed tagged forms of TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1) to determine where Tre6P is synthesized in arabidopsis (Arabidopsis thaliana), and investigated the impact of localized changes in Tre6P levels, in axillary buds or vascular tissues, on shoot branching in wild-type and branching mutant backgrounds. TPS1 is expressed in axillary buds and the subtending vasculature, as well as in the leaf and stem vasculature. Expression of a heterologous Tre6P phosphatase (TPP) to lower Tre6P in axillary buds strongly delayed bud outgrowth in long days and inhibited branching in short days. TPP expression in the vasculature also delayed lateral bud outgrowth and decreased branching. Increased Tre6P in the vasculature enhanced branching and was accompanied by higher expression of FLOWERING LOCUS T (FT) and upregulation of sucrose transporters. Increased vascular Tre6P levels enhanced branching in branched1 but not in ft mutant backgrounds. These results provide direct genetic evidence of a local role for Tre6P in regulation of axillary bud outgrowth within the buds themselves, and also connect Tre6P with systemic regulation of shoot branching via FT.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Localization and Function Of Tps1 In Arabidopsis Axillary Budsmentioning

confidence: 99%

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confidence: 99%

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Regulation of shoot branching in arabidopsis by trehalose 6‐phosphate

et al. 2020

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“…T6P directs primary metabolism toward a more consumptive mode, thus a cytokinin-induced increase would be consistent with the generally proliferative action of the hormone. However, it is not clear whether TPS1 (AT1G78580), which appears to encode the major T6P biosynthetic enzyme ( Fichtner et al, 2020 ), and the other enzymes involved in T6P homeostasis are directly regulated by the cytokinin-dependent TCS signaling network or whether the homeostatic regulation mentioned above is an indirect effect of altered sucrose levels due to cytokinin modulating carbohydrate consumption by, e.g., growth processes. Motifs that are demonstrated to bind type-B response regulators ( Franco-Zorrilla et al, 2014 ) or that are enriched in cytokinin-responsive promoters ( Brenner and Schmülling, 2015 ) are present in the promoter region of TPS1, favoring the idea of direct manipulation of T6P homeostasis and the associated changes in primary metabolism by cytokinin.…”

Section: Small Downstream Effectorsmentioning

confidence: 99%

Cytokinin Signaling Downstream of the His-Asp Phosphorelay Network: Cytokinin-Regulated Genes and Their Functions

Kroll

Brenner

2020

Front. Plant Sci.

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Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems

et al. 2021

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Trehalose is a non‐reducing disaccharide widely distributed in nature. The trehalose biosynthetic intermediate, trehalose 6‐phosphate (Tre6P) is an essential regulatory and signaling molecule involved in both regulation of carbon metabolism and photosynthesis. To investigate the effect of altered trehalose synthesis on sucrose accumulation in sugarcane (Saccharum spp. hybrid), we independently overexpressed the Escherichia coli otsA (trehalose‐6‐phosphate synthase; TPS) and otsB (trehalose‐6‐phosphate phosphatase; TPP) genes and additionally partially silenced native TPS expression. In mature cane, sucrose levels in the otsA transgenic plants were lowered, whereas sucrose levels in the otsB transgenic plants were increased. Partial silencing of TPS expression in sugarcane transformed with a TPS‐targeted microRNA recombinant construct was confirmed in leaf and mature internode tissue of transgenic plants. Most of the silencing transgenic lines accumulated trehalose at lower levels than the wild‐type (WT) plants. The immature stalk tissue of these transgenic lines had lower levels of glucose and fructose, whereas the mature internode tissue had lower sucrose and glucose levels, when compared with the WT. Furthermore, various minor metabolites and sugars were detected in the sugarcane plants, which mostly decreased as the stalk tissue of the cane matured. The results demonstrate that manipulation of Tre6P/trehalose metabolism has the potential to modify the profile of soluble sugars accumulated in sugarcane stems.

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Functional Features of TREHALOSE-6-PHOSPHATE SYNTHASE1, an Essential Enzyme in Arabidopsis[OPEN]

Abstract: Tre6P synthesis by TPS1 is essential for embryogenesis and postembryonic growth in Arabidopsis, and appropriate sucrose signaling by Tre6P is dependent on the non-catalytic domains of TPS1.

Cited by 89 publications

References 74 publications

Regulation of shoot branching in arabidopsis by trehalose 6‐phosphate

Regulation of shoot branching in arabidopsis by trehalose 6‐phosphate

Cytokinin Signaling Downstream of the His-Asp Phosphorelay Network: Cytokinin-Regulated Genes and Their Functions

Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems

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