Functional gait analysis in a spinal contusion rat model

Bhimani, Abhiraj D.; Kheirkhah, Pouyan; Arnone, Gregory D.; Nahhas, Cindy R.; Kumar, Prateek; Wonais, Matt; Hidrogo, Hugo; Aguilar, Eddy; Spalinski, Daniel; Gopalka, Mahie; Roth, Steven; Mehta, Ankit I.

doi:10.1016/j.neubiorev.2017.09.007

Cited by 28 publications

(17 citation statements)

References 26 publications

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“…This is in accordance with findings after sciatic nerve transection. It is safe to assume that femoral nerve resection does not impair hind limb‐forelimb coordination itself, as observable after spinal cord injuries, since the general pattern generator is located within the central nervous system (Alford & Schwartz, ; Bhimani et al, ; Hamers et al, ; Kyriakou et al, ). The distribution of NSSP was significantly altered after femoral nerve resection, with a significant decrease in Ab patterns.…”

Section: Discussionmentioning

confidence: 99%

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

2020

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Background:A great extent of knowledge on peripheral nerve regeneration has been gathered using the rat sciatic nerve model. The femoral nerve model of the rat offers an interesting alternative, as it lacks disadvantageous features such as automutilation. For the analysis of locomotor behavior in rats after sciatic nerve injury, the CatWalk ™ XT Gait Analysis System is often used. However, lesions of the femoral nerve in the rat have yet remained unstudied with this method. Material and Methods: Ten male Sprague Dawley rats were evaluated with theCatWalk XT to study their gait after a 6-mm resection of the right femoral nerve and reconstruction with an autologous nerve graft. Animals were observed for 10 weeks after surgery.Results: Print Area, Print Length, Swing Speed, and Duty Cycle decreased to a minimum of 40% of baseline 2 weeks after surgery. Swing Time was elevated more than twofold at this time point. However, all these parameters recovered back to >90% of baseline values at 10 weeks after surgery. This degree of functional recovery has not been reported after sciatic nerve resection and autograft repair. Base of support varied minimally postoperatively in contrast to a strong decrement after sciatic nerve resection and repair. Conclusion:We hereby provide a comprehensive in-depth analysis of how to study functional recovery after injury of the femoral nerve in the rat via the CatWalk XT.We place special emphasis on highlighting the differences between the femoral nerve and sciatic nerve injury model in this context. K E Y W O R D SCatWalk XT, femoral nerve, functional recovery, gait analysis, rats, sciatic nerve

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Section: Discussionmentioning

confidence: 99%

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

2020

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“…Similar schemes have been devised for use in dogs ( 38 , 39 ) and all carry the advantage of allowing the quality of locomotion to be assessed, so implying that grades of recovery can be measured. However, there are also drawbacks, most notably that these scores are not truly numerical (and so are ordinal rather than continuous scales), which complicates interpretation ( 40 ), and there is also a great deal of inter-animal variability in outcome, even in rats that have incurred highly-regulated identical injuries ( 41 ). In addition, although easily applied in practice, these scales are designed to detect a surrogate outcome—one that is collected for the purposes of a trial rather than to detect a useful clinical benefit.…”

Section: Outcome Assessmentmentioning

confidence: 99%

Clinical Trial Design—A Review—With Emphasis on Acute Intervertebral Disc Herniation

2020

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There is a clear need for new methods of treatment of acute disc herniation in dogs, most obviously to address the permanent loss of function that can arise because of the associated spinal cord injury. Clinical trials form the optimal method to introduce new therapies into everyday clinical practice because they are a reliable source of unbiased evidence of effectiveness. Although many designs are available, parallel cohort trials are most widely applicable to acute disc herniation in dogs. In this review another key trial design decision—that between pragmatic and explanatory approaches—is highlighted and used as a theme to illustrate the close relationship between trial objective and design. Acute disc herniation, and acute spinal cord injury, is common in dogs and there is a multitude of candidate interventions that could be trialed. Most current obstacles to large-scale clinical trials in dogs can be overcome by collaboration and cooperation amongst interested veterinarians.

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“…Preclinical rat SCI studies used in developing treatments for SCI (Ahuja et al, 2017;Cheriyan et al, 2014;Kjell and Olson, 2016;Silva et al, 2014) commonly rely on a motor assessment based upon a standardized scale involving the observation of various characteristics to generate a non-parametric score (Bhimani et al, 2017). For rat thoracic contusion SCI models, in particular, Basso et al developed a standard nonparametric locomotion assessment named "Basso Beattie Bresnahan (BBB) locomotion scale" (Basso et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Combination of Defined CatWalk Gait Parameters for Predictive Locomotion Recovery in Experimental Spinal Cord Injury Rat Models

Timotius

Bieler

Couillard‐Després

et al. 2021

eNeuro

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Functional gait analysis in a spinal contusion rat model

Cited by 28 publications

References 26 publications

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

Clinical Trial Design—A Review—With Emphasis on Acute Intervertebral Disc Herniation

Combination of Defined CatWalk Gait Parameters for Predictive Locomotion Recovery in Experimental Spinal Cord Injury Rat Models

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