Functional Genomics of Metal-Reducing Microbes Degrading Hydrocarbons

Tremblay, Pier‐Luc; Zhang, Tian

doi:10.1007/978-3-319-50391-2_13

Cited by 2 publications

(2 citation statements)

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“…On average, sequences classified as genera associated with FeRB (Supplementary Table S2) totaled 0.755 and 0.971% relative abundance of sequences from hydrocarbon-exposed and unexposed iron mats, respectively. Of these, the genus Geobacter has the potential to couple iron reduction with benzene degradation (Tremblay and Zhang, 2020) and represented an average of 0.56 and 0.74% relative abundance of hydrocarbon-exposed and unexposed iron mats, respectively. Sequences classified as genera associated with SRB (Supplementary Table S3), on average, 0.143 and 0.209% relative abundance of hydrocarbon-exposed and hydrocarbon-unexposed iron mats, respectively.…”

Section: Figurementioning

confidence: 99%

“…The chemical properties of iron mats are not the only source of potential for benzene removal. There have been multiple studies that show involvement in the biodegradation of benzene and other hydrocarbons from functional groups such as sulfate-reducing bacteria (SRB) (Edwards and Grbić-Galić, 1992;Chakraborty and Coates, 2004;Keller et al, 2018), iron-reducing bacteria (FeRB) (Lovley et al, 1996;Jahn et al, 2005;Tremblay and Zhang, 2020), and nitrate-reducing bacteria (NRB) (Coates et al, 2001;Fahy et al, 2008;Atashgahi et al, 2018), which have been previously identified in iron mats. If hydrocarbon-degrading organisms are present and active, the iron mat community could prove to be an invaluable resource in application of hydrocarbon remediation at the oxic-anoxic boundary.…”

Section: Introductionmentioning

confidence: 99%

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Microbial community response to hydrocarbon exposure in iron oxide mats: an environmental study

Brooks,

Field

2024

Front. Microbiol.

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Hydrocarbon pollution is a widespread issue in both groundwater and surface-water systems; however, research on remediation at the interface of these two systems is limited. This interface is the oxic–anoxic boundary, where hydrocarbon pollutant from contaminated groundwaters flows into surface waters and iron mats are formed by microaerophilic iron-oxidizing bacteria. Iron mats are highly chemically adsorptive and host a diverse community of microbes. To elucidate the effect of hydrocarbon exposure on iron mat geochemistry and microbial community structure and function, we sampled iron mats both upstream and downstream from a leaking underground storage tank. Hydrocarbon-exposed iron mats had significantly higher concentrations of oxidized iron and significantly lower dissolved organic carbon and total dissolved phosphate than unexposed iron mats. A strong negative correlation between dissolved phosphate and benzene was observed in the hydrocarbon-exposed iron mats and water samples. There were positive correlations between iron and other hydrocarbons with benzene in the hydrocarbon-exposed iron mats, which was unique from water samples. The hydrocarbon-exposed iron mats represented two types, flocculent and seep, which had significantly different concentrations of iron, hydrocarbons, and phosphate, indicating that iron mat is also an important context in studies of freshwater mats. Using constrained ordination, we found the best predictors for community structure to be dissolved oxygen, pH, and benzene. Alpha diversity and evenness were significantly lower in hydrocarbon-exposed iron mats than unexposed mats. Using 16S rDNA amplicon sequences, we found evidence of three putative nitrate-reducing iron-oxidizing taxa in microaerophile-dominated iron mats (Azospira, Paracoccus, and Thermomonas). 16S rDNA amplicons also indicated the presence of taxa that are associated with hydrocarbon degradation. Benzene remediation-associated genes were found using metagenomic analysis both in exposed and unexposed iron mats. Furthermore, the results indicated that season (summer vs. spring) exacerbates the negative effect of hydrocarbon exposure on community diversity and evenness and led to the increased abundance of numerous OTUs. This study represents the first of its kind to attempt to understand how contaminant exposure, specifically hydrocarbons, influences the geochemistry and microbial community of freshwater iron mats and further develops our understanding of hydrocarbon remediation at the land–water interface.

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Section: Figurementioning

confidence: 99%