Functional genomics of reproduction in pigs: Are we there yet?

Nonneman, Dan; Lents, C. A.

doi:10.1002/mrd.23625

Cited by 7 publications

(3 citation statements)

References 84 publications

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“…It has the characteristics of high frequency and stability, and SNP analysis is easier to automate and batch. 13 Therefore, using SNP as a genetic marker to screen for T2DM susceptibility genes is a very effective high-efficiency, low-cost method. The GWAS study of human T2DM has accumulated hundreds of related gene loci, such as TCF7L2, PPARG, and KCNJ11.…”

Section: Discussionmentioning

confidence: 99%

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Whole‐genome sequencing study to identify candidate markers indicating susceptibility to type 2 diabetes in Bama miniature pigs

Niu

Zhao

Jia

et al. 2023

Anim Models and Exp Med

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BackgroundHundreds of single‐nucleotide polymorphism (SNP) sites have been found to be potential genetic markers of type 2 diabetes mellitus (T2DM). However, SNPs related to T2DM in minipigs have been less reported. This study aimed to screen the T2DM‐susceptible candidate SNP loci in Bama minipigs so as to improve the success rate of the minipig T2DM model.MethodsThe genomic DNAs of three Bama minipigs with T2DM, six sibling low‐susceptibility minipigs with T2DM, and three normal control minipigs were compared by whole‐genome sequencing. The T2DM Bama minipig‐specific loci were obtained, and their functions were annotated. Meanwhile, the Biomart software was used to perform homology alignment with T2DM‐related loci obtained from the human genome‐wide association study to screen candidate SNP markers for T2DM in Bama miniature pigs.ResultsWhole‐genome resequencing detected 6960 specific loci in the minipigs with T2DM, and 13 loci corresponding to 9 diabetes‐related genes were selected. Further, a set of 122 specific loci in 69 orthologous genes of human T2DM candidate genes were obtained in the pigs. Collectively, a batch of T2DM‐susceptible candidate SNP markers in Bama minipigs, covering 16 genes and 135 loci, was established.ConclusionsWhole‐genome sequencing and comparative genomics analysis of the orthologous genes in pigs that corresponded to the human T2DM‐related variant loci successfully screened out T2DM‐susceptible candidate markers in Bama miniature pigs. Using these loci to predict the susceptibility of the pigs before constructing an animal model of T2DM may help to establish an ideal animal model.

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Section: Discussionmentioning

confidence: 99%

“…SNP is part of a new generation of genetic markers. It has the characteristics of high frequency and stability, and SNP analysis is easier to automate and batch 13 . Therefore, using SNP as a genetic marker to screen for T2DM susceptibility genes is a very effective high‐efficiency, low‐cost method.…”

Section: Discussionmentioning

confidence: 99%

Whole‐genome sequencing study to identify candidate markers indicating susceptibility to type 2 diabetes in Bama miniature pigs

Niu

Zhao

Jia

et al. 2023

Anim Models and Exp Med

View full text Add to dashboard Cite

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“…genes have been pinpointed to date, using genome-wide genotyping (GWG) and genome-wide association studies (GWAS) [13][14].…”

Section: Of 11mentioning

confidence: 99%

Associations Between RBP4, Fshb and Egf Gene Polymorphisms and Reproductive Traits in Pigs

Kowalewska¹,

Terman²,

Chomczyńska³

et al. 2023

Preprint

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Genes encoding retinol binding protein 4 (RBP4), follicle stimulating hormone subunit beta (FSHB) and epidermal growth factor (EGF) have been proposed as candidate genes for reproduction traits in pigs. The study presented in this paper aimed to find associations between variants of these genes and reproduction traits in pigs reared in Poland. Investigation included Polish Large White x Polish Landrace (n=288) and Yorkshire (n=195) sows. Individual genotypes were determined by means of PCR-RFLP (single nucleotide polymorphism in RBP4, FSHB) or PCR (insertion/deletion in EGF) methods. Obtained result showed that RBP4 variants were related to total number born (TNB) and number born alive (NBA) in 1st, 2nd and 5th parities, FSHB to TNB and NBA in first two parities, however EGF with both traits in all parities (P≤0.05 or P≤0.01). In case of RBP4 gene, heterozygous genotype (AB) was favorable for analyzed traits, however in case of FSHB and EGF homozygous genotype, AA and BB respectively. Obtained results indicate that polymorphism in three analyzed genes is associated with important reproductive traits of pigs kept in Poland.

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