Functional genomics studies of oocyte competence: evidence that reduced transcript abundance for follistatin is associated with poor developmental competence of bovine oocytes

Patel, Osman V.; Bettegowda, Anilkumar; Ireland, James J.; Coussens, Paul M.; Lonergan, Patrick; Smith, George W.

doi:10.1530/rep.1.01123

Cited by 109 publications

(131 citation statements)

References 65 publications

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“…The widespread distribution pattern of the follistatin-activin-activin receptor system in goat ovaries points to a crucial role of this system in various reproductive processes, including follicle growth and differentiation, and luteal activity. However, there are limited data on goats and on prepubertal animals (Brawtal, 1994;Zhao et al, 2001;Patel et al, 2007) about the expression of protein and mRNA for activin-A and activin receptors. Therefore, it is necessary to determine whether activin-A and its receptors are present in the embryos from the zygote to the morula stage to improve the results on in vitro embryo development of prepubertal goat oocytes.…”

Section: Discussionmentioning

confidence: 99%

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Hammami

Izquierdo

Catalá

et al. 2014

Animal

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The present study was designed to evaluate the effect of activin-A during the in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC) on nuclear maturation, blastocyst yield and blastocyst quality of prepubertal goat oocytes. In Experiment 1, three groups of oocytes were used during the IVM of prepubertal goat oocytes to determine the optimal concentration of recombinant human activin-A added to the maturation medium. Cumulus-oocyte complexes were matured in an IVM medium containing 0, 10 and 100 ng/ml (groups A0, A10 and A100), fertilized and in vitro cultured using standard procedures. In Experiment 2, the addition of 10 ng/ml activin-A at IVM (A10A0), IVC (A0A10) or IVM + IVC (A10A10) was studied and compared with the control group (A0A0). Results of the first experiment demonstrated that the addition of activin-A yielded similar percentages of maturation (⩽71.0%) and blastocyst formation rates (⩽24.9%) than the control group (A0). Experiment 2 showed that exposure of prepubertal goat oocytes to an IVC medium containing 10 ng/ml activin-A (A0A10) significantly increased the rates of development to the blastocyst stage, as compared with the control group (A0A0) (19.5 ± 2.21% v. 13.1 ± 2.37%, respectively; P < 0.05). With regard to the blastocyst quality, total number of cells, inner cell mass (ICM) and trophectoderm of prepubertal goat embryos produced in the presence of activin-A did not differ significantly among experimental groups. In summary, these results indicate that supplementation of the IVC medium with activin-A enhances embryo development of prepubertal goat oocytes.

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Section: Discussionmentioning

confidence: 99%

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Hammami

Izquierdo

Catalá

et al. 2014

Animal

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“…On the other hand, PTX3 and IGFII mRNA were not different between the follicle size categories [81]. Patel et al [82] reported a higher mRNA content of the βA-subunit of inhibin/activin in CCs collected from adult cows compared with prepubertal cows.…”

Section: Body Condition (Age Body Weight Genotype and Reproductive mentioning

confidence: 92%

Factors Influencing the Biochemical Markers for Predicting Mammalian Oocyte Quality

Ola

Sun

2012

Journal of Reproduction and Development

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Abstract. The need for accurate selection of the best oocytes for in vitro fertilization protocols and thus, production of embryos has driven the search for oocyte quality markers from morphological criteria to biochemical parameters. Current studies are focused on the biochemical constituents of the follicular fluid and gene expression profiling of the cumulus cells. These parameters are, however, affected by factors that must be considered before making a judgment of the oocyte's quality. These includes factors such as the type of hormonal stimulation protocol, age of oocyte donor and heat stress on the donor, all of which have been reported to influence the concentrations of many hormones, apolipoproteins, metabolites, fatty acids and growth factors in the follicular fluid and the expression of several genes in the cumulus cells. Another important point to note is species variation in the response to these extraneous influences, which thus calls for species targeted investigations. As reports are still scanty and investigations assumed to be very keen, we employed this review paper to bring attention of researchers and clinicians to those factors that may come to bear on the outcome of their investigations on oocyte and embryo quality. Key words: Cumulus cells, Follicular fluid, Mammalian oocytes, Quality prediction (J. Reprod. Dev. 58: [385][386][387][388][389][390][391][392] 2012) I t is well known that only part of the in vivo ovulated oocytes and in vitro matured oocytes are capable of developing to viable embryos after fertilization. This led to the search for a reliable indicator of oocyte quality. Thus, one of the important puzzles for reproductive biologists today is the search for a reliable noninvasive marker of the quality of oocytes for both human and animal assisted reproduction.Initial attempts at evaluating the quality of oocytes meant for in vitro fertilization (IVF) focused on the physical/morphological characteristics of the Cumulus-oocyte-complexes (COCs) [1,2]. These criteria are both noninvasive and rapid and are still generally in use today. However, recent reports on the inadequacies of a simple morphological appearance as a measure of quality suggest that morphological markers cannot be completely relied upon [3,4]. This has led to intensification of the search for alternative or complementary criteria with focus on the follicular fluid (FF) and the cumulus cells (CCs) around the oocyte.The preovulatory oocyte communicates intimately and directly with the surrounding cumulus granulosa cells through specialized transzonal cytoplasmic projections [5] and with the more distant mural granulosa cells through the follicular fluid [6]. Many biochemical substances found in these close associates of the oocyte have been studied and correlated with the developmental competence of the oocyte. Very recent attempts are even looking beyond a single substance to the totality of the biochemical constituents of the follicular fluid. This area of research is referred to as metabolomics [7]. Revelli e...

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“…Estudos com esse objetivo foram realizados por vários grupos de pesquisa, o que resultou na identificação de vários marcadores potenciais (Tabela 1), com diversas funções biológicas (Robert et al, 2000;Donnison and Pfeffer, 2004;Mourot et al, 2006;Pfeffer et al, 2006;Ghanem et al, 2007;Patel et al, 2007;Racedo et al, 2008;Caixeta et al, 2009;Bessa et al, 2013). Apesar desse tipo de estudo fornecer informações importante para o entendimento sobre a regulação da capacidade de desenvolvimento, é necessário a destruição do ovócito para realizar a análise molecular.…”

Section: Marcadores No Ovócitounclassified

“…Estágio de maturação Referência CCNB1 VG (Robert et al, 2000) CCNB2, CKS1B, CDC5L, PSMB2, SKIIP, RGS16, PRDX1 VG (Mourot et al, 2006) CCMA2, NDFIP1, OCT4, MSX1, ZNF198, SLBP VG (Donnison and Pfeffer, 2004) DNAJA1 (DJA4), GDF9, TRAPPC3 VG (Pfeffer et al, 2007) DYNLL1, DYNC1I1 VG (Racedo et al, 2008) PTTG1 VG (Mourot et al, 2006;Ghanem et al, 2007) H2A VG (Caixeta et al, 2009) RPL24, MSX1 VG (Ghanem et al, 2007) INHBA, INHBB VG (Patel et al, 2007) PRDX1, PRDX2 VG (Romar et al, 2011) G6PDH VG (Ghanem et al, 2007) RBM42, LSM10, HAUS8, AURKAIP1, CDK1, PAIP2, ENY2, ESCO2, PMS1, ELP4, TFTP1, SFRS7, TAF1A…”

Section: Genementioning

confidence: 99%

Marcadores moleculares para qualidade de ovocitos bovino

Sprícigo¹,

Dode²

2017

SPERMOVA

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A seleção de ovócitos baseada em critérios morfológicos tem sido o método utilizado até o presente, no entanto, esse método apresenta limitações quanto a acurácia, objetividade e constância. Dessa forma, existe a necessidade de se buscar outras ferramentas que permitam uma seleção mais acurada de ovócitos competentes. Considerando que o ambiente folicular pode refletir características do ovócito, esse pode fornecer alternativas para a avaliação não invasiva da qualidade do ovócito. Nesse sentido, os esforços estão concentrados no perfil transcricional de células do cumulus (CC) e células da granulosa (CGM) e no perfil bioquímico do liquido folicular (LF). Entre as abordagens estão a análise do transcriptoma, proteoma e metaboloma. Nessa revisão inicialmente são descritos estudos que visam identificar marcadores no próprio ovócito, que apesar de fornecerem informações importante para o entendimento sobre a regulação da capacidade de desenvolvimento, inviabilizam o uso subsequente do ovócito. Posteriormente, são revisados os estudos que avaliam a análise transcricional das CCs e da CMG em correlação com a competência do ovócito e, em especial, os que acompanham o desenvolvimento embrionário para validar marcadores. E, finalmente é abordada a possibilidade de se identificar marcadores bioquímicos no LF e a tendência dos últimos anos, que tem passado de estudo marcadores únicos para técnicas mais complexas que estudam todos os metabólitos de LF utilizando a metabolômica como ferramenta. Palavra-chave: competência, ovócito, célula do cumulus, líquido folicular. ABSTRACTOocyte selection using morphological criteria is currently used, but this selection approach has limitations in terms of accuracy, objectivity, and constancy. Therefore, the development of new methods that could allow the identification of molecular markers that can predict the oocyte competency more accurately is needed. Considering that follicular environment may reflects oocyte status, it can provide new alternatives for non-invasive assessment of oocyte quality. The efforts are concentrated on determining transcriptional profiling of cumulus (CC) and granulosa cells (MGC) and on biochemical profiling of follicular fluid (FF). The approaches used are transcriptomics, proteomics, and metabolomics technologies. In this review, we first describe the studies that aimed to identify molecular markers on the oocyte itself, that although gives relevant information regarding to the understanding of competency acquisition, they destroy the oocyte. Then, studies that evaluated cumulus/granulosa cell gene expression and especially those that followed the embryo development until the blastocyst stage are reported. Lastly, the possibility of identifying markers on FF is discussed as well as the tendency observer on the last few years of evaluating not only one metabolite but all metabolites using metabolomics as tool.

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Functional genomics studies of oocyte competence: evidence that reduced transcript abundance for follistatin is associated with poor developmental competence of bovine oocytes

Cited by 109 publications

References 65 publications

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Factors Influencing the Biochemical Markers for Predicting Mammalian Oocyte Quality

Marcadores moleculares para qualidade de ovocitos bovino

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