Functional hepatic recovery after xenotransplantation of cryopreserved fetal liver cells or soluble cell‐factor administration in a cirrhotic rat model: Are viable cells necessary?

Ochenashko, O. V.; Nikitchenko, Yurii V; Волкова, Н. А.; Mazur, Svitlana; Somov, Alexander Y.; Fuller, Barry; Petrenko, Alexander

doi:10.1111/j.1440-1746.2007.05095.x

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…We presume that transplanted cells secrete growth factors or similar agents that regulate the metabolism and proliferation of host hepatocytes. Ochenashko et al (2008) found that human fetal liver cells and cell-free fetalspecific factors of nonhepatic origin stimulated the recovery processes in an experimental model of CCl 4 -induced cirrhosis in rats. These findings indicate that such effect may result from the presence of stage-specific factors that activate hepatocellular repair.…”

Section: Discussionmentioning

confidence: 98%

Human amniotic fluid-derived stem cells can differentiate into hepatocyte-like cells in vitro and in vivo

Liu

Liu²,

Li³

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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Although human amniotic fluid is an attractive source of multipotent stem cells, the potential of amniotic fluid stem cells (AFSCs) to differentiate into hepatic cells has not been extensively evaluated. In this study, we examined whether human AFSCs can differentiate into a hepatic cell lineage in vitro and in vivo. After being treated with cytokines (fibroblast growth factor 4, basic fibroblast growth factor, hepatocyte growth factor, and oncostatin), AFSCs developed a morphology similar to that of hepatocytes. RT-PCR and immunofluorescence analysis showed that the treated AFSCs expressed the hepatocyte-specific markers albumin, cytokeratin 18, and alpha-fetoprotein. The differentiated cells also developed hepatocyte-specific functions, i.e., they secreted albumin, absorbed indocyanine green, and stored glycogen. When transplanted into CCl(4)-injured immunodeficient mice, undifferentiated AFSCs were integrated into the liver tissue, and they expressed markers characteristic of mature human hepatocytes. Although integration of AFSCs into the liver was limited (0.1-0.3% of hepatocytes), histological analysis showed that the recipient mice recovered more rapidly from CCl(4) injury than CCl(4)-injured mice that did not receive AFSCs. AFSCs can differentiate into hepatocyte-like cells in vitro and in vivo and can represent an easily accessible source of progenitor cells for hepatocyte regeneration and liver cell transplantation.

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Section: Discussionmentioning

confidence: 98%

Human amniotic fluid-derived stem cells can differentiate into hepatocyte-like cells in vitro and in vivo

Liu

Liu²,

Li³

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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“…The effective concentration for BSPC was established in a set of preliminary dose-ranging experiments in which livers were flushed with SPS and a range of bioregulator concenta-tions (10,20,30, and 40 lg of protein/mL of SPS), preserved for 24 h and reperfused.…”

Section: The Experimental Modelmentioning

confidence: 99%

“…In our previous studies the effects of bioregulators of stem and progenitor cells (BSPC) contained in fetal tissue cytosol of mesenchymal-mesodermal origin in several of experimental pathologies were shown [9][10][11]. Our choice of this cell type was due to their well-known unique capacity to produce a wide range of biologically active substances that affect the intra-and extracellular processes [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Bioregulators of stem and progenitor cells in preservation solution reduce cold ischemia‐reperfusion injury of isolated rat livers

et al. 2016

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The effects of bioregulators of stem and progenitor cells (BSPC) of fetal tissue cytosol on rat liver during 24h hypothermic storage (HS) and following normothermic reperfusion (NR) were investigated. It was shown that BSPC presence in the preservation medium stabilized pro‐oxidant‐antioxidant balance impaired in liver after HS and NR, prevented the uncoupling of mitochondrial oxidative phosphorylation and ATP level decline. These consequences led to significant improvement of hepatic morphological integrity and functional state. Powerful protective effect of BSPC on liver at sub‐zero temperatures can serve as basis for new approaches to extend safe time for organ preservation and foster understanding of pathways of stem and progenitor cell paracrine action. © 2016 BioFactors, 42(3):287–295, 2016

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“…морфологии печени. Схожие изменения нами были установлены ранее при инъекционном введении криоконсервированных КФП в селезенку крыс с эк-спериментальным циррозом [18]. В совокупности эти данные указывают на высокий терапевтический потенциал КФП.…”

Section: результаты и обсуждениеunclassified

Transplantation of Cryopreserved Fetal Liver Cells Seeded Into Macroporous Alginate-Gelatin Scaffolds in Rats With Liver Failure

Грицай

Лебединский

Ochenashko

et al. 2015

RJTAO

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1 Институт проблем криобиологии и криомедицины НАН Украины, Харьков, Украина 2 Институт элементоорганических соединений им. А.Н. Несмеянова РАН, Москва, Российская Федерация Цель. Изучить терапевтический потенциал криоконсервированных клеток фетальной печени, заселенных в макропористые альгинат-желатиновые матрицы, при имплантации в сальник крыс с печеночной недо-статочностью. Материалы и методы. Печеночную недостаточность индуцировали введением 2-ацетил-аминофлуорена с последующим проведением частичной гепатэктомии. В сальник крыс имплантировали макропористые альгинат-желатиновые матрицы, заселенные криоконсервированными аллогенными клет-ками фетальной печени (КФП). Матрицы дополнительно покрывали альгинатной оболочкой (для предот-вращения заселения клетками хозяина). Длительность эксперимента после формирования модели и имп-лантации составила 4 нед. В крови определяли ряд гепатоспецифических показателей, морфологию печени исследовали гистологически. Клетки, заселенные в альгинат-желатиновые матрицы, визуализировали флу-оресцентным красителем, состояние матриц после имплантации оценивали гистологически. Результаты. Имплантация макропористых матриц здоровым крысам сопровождалась их интенсивным заселением клет-ками хозяина. Нанесение дополнительной капсулы из альгината на матрицу практически предотвращало это явление. Имплантация макропористых матриц, заселенных криоконсервированными КФП и покрытых альгинатным гелем, животным с печеночной недостаточностью приводила к значительному улучшению гепатоспецифических показателей крови в печени крыс и сопровождалась позитивными изменениями мор-фологии печени экспериментальных животных. Через 4 нед. в имплантированных матрицах были выявле-ны клетки и образованный ими экстраклеточный матрикс. Заключение. Приведенные результаты свиде-тельствуют, что широкопористые альгинат-желатиновые матрицы, заключенные в оболочку из альгината, являются перспективными носителями клеток для разработки биоинженерных эквивалентов печени.Ключевые слова: клетки фетальной печени, альгинат-желатиновые матрицы, имплантация, печеночная недостаточность. TRANSPLANTATION OF CRYOPRESERVED FETAL LIVER CELLS SEEDED INTO MACROPOROUS ALGINATE-GELATIN SCAFFOLDS IN RATS WITH LIVER FAILURE

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Functional hepatic recovery after xenotransplantation of cryopreserved fetal liver cells or soluble cell‐factor administration in a cirrhotic rat model: Are viable cells necessary?

Abstract: The positive effects of fetal liver cell and cell-free fetal-specific factors in experimental cirrhosis may result from the presence of stage-specific factors activating hepatocellular repair.

Cited by 5 publications

References 39 publications

Human amniotic fluid-derived stem cells can differentiate into hepatocyte-like cells in vitro and in vivo

Human amniotic fluid-derived stem cells can differentiate into hepatocyte-like cells in vitro and in vivo

Bioregulators of stem and progenitor cells in preservation solution reduce cold ischemia‐reperfusion injury of isolated rat livers

Transplantation of Cryopreserved Fetal Liver Cells Seeded Into Macroporous Alginate-Gelatin Scaffolds in Rats With Liver Failure

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