Functional identification of MdMYB5 involved in secondary cell wall formation in apple

Chen, Keqin; Tang, Xiaoguang; Song, Mengru; Guo, Yunna; Liu, Lifu; Hou, Xue; Hirata, Dai; Zhang, Zhihong

doi:10.48130/frures-2021-0006

Cited by 10 publications

(10 citation statements)

References 38 publications

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“…Reports show that the regulatory effects on flavonoids metabolism by MYB5 TFs are inconsistent across plants, with some MYB5 TFs promoting both PA and anthocyanin accumulation (Deluc et al ., 2008; Li et al ., 2019), others promoting PA but inhibiting or not regulating anthocyanins (Jiang et al ., 2018) and still others regulating only individual intermediate in the flavonoid pathway (Arlotta et al ., 2020). In addition, MYB5 TFs also regulate leaf trichome morphology and secondary cell wall formation (Chen et al ., 2021; Deluc et al ., 2008; Li et al ., 2009). Recently, FaMYB5 was shown to regulate citric acid metabolism in fruits (Liu et al ., 2022b).…”

Section: Discussionmentioning

confidence: 99%

“…The apple MdMYB5 was related to secondary cell wall formation. Ectopic overexpression of MdMYB5 in Arabidopsis increased plant lignin and cellulose content (Chen et al ., 2021). Nevertheless, ectopic expression of VvMYB5a and VvMYB5b in tobacco significantly promoted the accumulation of anthocyanin and PA in petals (Deluc et al ., 2005, 2008).…”

Section: Introductionmentioning

confidence: 99%

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A novel R2R3‐MYB transcription factor FaMYB5 positively regulates anthocyanin and proanthocyanidin biosynthesis in cultivated strawberries (Fragaria × ananassa)

Jiang

Yue

Liu

et al. 2023

Plant Biotechnology Journal

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Flavonoids have a major contribution to the fruit quality in cultivated strawberries and are regulated by MYB, bHLH and WD40 transcriptional factors. We reported here the identification of the FaMYB5, an R2R3-MYB transcription factor, which positively regulated the accumulation of anthocyanins and proanthocyanidins through the trans-activation of the F3'H and LAR. The strawberry FaEGL3 and FaLWD1/FaLWD1-like interact with the R2R3-FaMYB5 to form an MYB-bHLH-WD40 complex (MBW), enhancing the regulatory efficiency. The R2R3-FaMYB5 was constitutively expressed in various tissues and in fruits of different developmental stages, which was strikingly contrasting to the fruit-specific expression patterns of FaMYB10. Meanwhile, R2R3-FaMYB5 failed to promote a stable accumulation of anthocyanin glycosides in the mature fruits of the myb10 mutant, mainly due to the suppressed expression of TT19. The R2R3-FaMYB5 was regulated by an antisense long noncoding RNA lncRNA-myb5. Additionally, the R2R3-FaMYB5 protein could interact with FaBT2 and was degraded through the ubiquitin/26 S proteasome pathway. Transcriptome and metabolome data showed that R2R3-FaMYB5 enhanced the gene expression and the metabolite accumulation involved in the flavonoid, phenylpropanoid and lignin biosynthesis pathways. Collectively, we conclude that the FaMYB5 is an R2R3-MYB activator involved in the composition of MBW, which positively regulates the biosynthesis of anthocyanin and proanthocyanidin. These findings provided new insights into the molecular mechanisms that regulate flavonoids in strawberry fruits.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel R2R3‐MYB transcription factor FaMYB5 positively regulates anthocyanin and proanthocyanidin biosynthesis in cultivated strawberries (Fragaria × ananassa)

Jiang

Yue

Liu

et al. 2023

Plant Biotechnology Journal

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“…4). To date, many MYB TFs, such as MYB5, MYB96, MYB63, MYB46, MYB91, MYB15, and MYB2, have been found to be involved in ABA and/or abiotic stress responses (Ding et al, 2009;Seo et al, 2009;Guo et al, 2013;Chen et al, 2019;Chen et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Transcription factors (TFs) play a crucial role in how plants react to ABA and shifting environmental conditions (Shinozaki et al, 2003;Sah et al, 2016). A genetic network for stress adaptation comprises various types of TFs, such as MYC, WRKY, NAC, bZIP, and MYB, which affect downstream gene expression levels either dependently or independently (Kang et al, 2002;Abe et al, 2003;Tran et al, 2004;Chen et al, 2012;Rushton et al, 2012;Chen et al, 2019;Chen et al, 2021). MYB TFs are categorized into 4 groups based on the amount and type of MYB domain repeats: 1R-, R2R3-, 3R-, and 4R-MYB Li et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

MdMYB44-like positively regulates salt and drought tolerance via the MdPYL8-MdPP2CA module in apple

Zhang

Lei

Zhang

et al. 2023

Preprint

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Abscisic acid (ABA) is involved in salt and drought stress responses, but the underlying molecular mechanism remains unclear. Here, we demonstrated that the overexpression of MdMYB44-like, an R2R3-MYB transcription factor (TF), significantly increases the salt and drought tolerance of transgenic apple and Arabidopsis. MdMYB44-like inhibits the transcription of MdPP2CA, which encodes a type 2C protein phosphatase that acts as a negative regulator in ABA response, thereby enhancing ABA signaling-mediated salt and drought tolerance. Furthermore, we found that MdMYB44-like and MdPYL8, an ABA receptor, form a protein complex that further enhances the transcriptional inhibition of the MdPP2CA promoter by MdMYB44-like. Significantly, we discovered that MdPP2CA can interfere with the physical association between MdMYB44-like and MdPYL8 in the presence of ABA, partially blocking the inhibitory effect of the MdMYB44-like-MdPYL8 complex on the MdPP2CA promoter. Thus, MdMYB44-like, MdPYL8, and MdPP2CA form a regulatory loop that tightly controls ABA signaling homeostasis under salt and drought stress. Our data revealed a previously unidentified mechanism by which MdMYB44-like precisely modulates ABA-mediated salt and drought tolerance in apple through the MdPYL8-MdPP2CA module.

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“…There were three biological and technical replicates. Relative expression levels of all examined genes and small RNA were calculated using the 2 −ΔΔCt method [ 63 ].…”

Section: Methodsmentioning

confidence: 99%

MIR390 Is Involved in Regulating Anthracnose Resistance in Apple

Shi

Jiang

Zhang

et al. 2022

Plants

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As an important cash crop in China, apple has a good flavor and is rich in nutrients. Fungal attacks have become a major obstacle in apple cultivation. Colletotrichum gloeosporioides is one of the most devastating fungal pathogens in apple. Thus, discovering resistance genes in response to C. gloeosporioides may aid in designing safer control strategies and facilitate the development of apple resistance breeding. A previous study reported that ‘Hanfu’ autotetraploid apple displayed higher C. gloeosporioides resistance than ‘Hanfu’ apple, and the expression level of mdm-MIR390b was significantly upregulated in autotetraploid plants compared to that in ‘Hanfu’ plants, as demonstrated by digital gene expression (DGE) analysis. It is still unclear, however, whether mdm-MIR390b regulates apple anthracnose resistance. Apple MIR390b was transformed into apple ‘GL-3′ plants to identify the functions of mdm-MIR390b in anthracnose resistance. C. gloeosporioides treatment analysis indicated that the overexpression of mdm-MIR390b reduced fungal damage to apple leaves and fruit. Physiology analysis showed that mdm-MIR390b increased C. gloeosporioides resistance by improving superoxide dismutase (SOD) and peroxidase (POD) activity to alleviate the damage caused by O2− and H2O2. Our results demonstrate that mdm-MIR390b can improve apple plants’ anthracnose resistance.

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Functional identification of MdMYB5 involved in secondary cell wall formation in apple

Cited by 10 publications

References 38 publications

A novel R2R3‐MYB transcription factor FaMYB5 positively regulates anthocyanin and proanthocyanidin biosynthesis in cultivated strawberries (Fragaria × ananassa)

A novel R2R3‐MYB transcription factor FaMYB5 positively regulates anthocyanin and proanthocyanidin biosynthesis in cultivated strawberries (Fragaria × ananassa)

MdMYB44-like positively regulates salt and drought tolerance via the MdPYL8-MdPP2CA module in apple

MIR390 Is Involved in Regulating Anthracnose Resistance in Apple

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