Functional Imaging of Colonic Mucosa With a Fibered Confocal Microscope for Real-Time In Vivo Pathology

Wang, Thomas D.; Friedland, Shai; Sahbaie, Peyman; Soetikno, Roy; Hsiung, Pei–Lin; Liu, Jonathan; Crawford, James M.; Contag, Christopher H.

doi:10.1016/j.cgh.2007.07.013

Cited by 109 publications

(76 citation statements)

References 27 publications

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“…A non-invasive method of diagnosis could remove the need for physical biopsy and provide improvements in diagnostic accuracy for a number of diseases. Although several novel imaging techniques that can be operated in conjunction with standard WLE have recently become available in the clinical setting -such as narrow-band imaging (NBI) [8], autofluorescence imaging (AFI) [9] and more recently confocal endomicroscopy (CLE) [10,11] -there is still no red flag technique for detection of GI premalignant lesions that can progress into cancer. Furthermore, there is currently no technology that can reliably indicate when it is appropriate not to biopsy.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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Abstract:We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens. References and links1. R. Lambert, H. Saito, and Y. Saito, "High-resolution endoscopy and early gastrointestinal cancer...dawn in the East," Endoscopy 39(3), 232-237 (2007

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Section: Introductionmentioning

confidence: 99%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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“…inverse contrast [31,32]. On the other hand, the opposite effect -enhanced penetration and retention (EPR) in tumors versus normal tissues -is also often observed [33][34][35]. Regardless of the functional mechanisms for the accumulation of contrast agents in tissues, there is increasing agreement that utilizing a dual-reporter ratiometric detection strategy, in which one negative reporter serves as a control for a simultaneously delivered positive probe, is valuable to accurately identify and quantify molecularly specific binding [23,31,32,34].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive spectral endoscopy of topically applied SERS nanoparticles in the rat esophagus

Wang

Khan

Leigh

et al. 2014

Biomed. Opt. Express

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Abstract:The early detection and biological investigation of esophageal cancer would benefit from the development of advanced imaging techniques to screen for the molecular changes that precede and accompany the onset of cancer. Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have the potential to improve cancer detection and the investigation of cancer progression through the sensitive and multiplexed phenotyping of cell-surface biomarkers. Here, a miniature endoscope featuring rotational scanning and axial pull back has been developed for 2D spectral imaging of SERS NPs topically applied on the lumenal surface of the rat esophagus. Raman signals from low-pM concentrations of SERS NP mixtures are demultiplexed in real time to accurately calculate the concentration and ratio of the NPs. Ex vivo and in vivo experiments demonstrate the feasibility of topical application and imaging of multiplexed SERS NPs along the entire length of the rat esophagus.

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“…Other topical dyes (or precursors) that have been used for CLE are cresyl violet, hypericin, 5-aminolevulinic acid, topical fluorescein and 2-NBDG (2-[N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl)amino]-2-deoxyglucose) [26][27][28][29][30].…”

Section: Fluorescent Contrast Agents For Confocal Laser Endomicroscopymentioning

confidence: 99%

Confocal laser endomicroscopy in head and neck cancer

Volgger¹,

Conderman

Betz³

2013

Current Opinion in Otolaryngology & Head and Neck Surgery

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Purpose of reviewConfocal laser endomicroscopy (CLE) is a novel, noninvasive technique used to obtain microanatomical images of the inner lining of hollow organs. It has been used in a variety of clinical specialties to aid in the diagnosis and treatment planning of inflammatory and neoplastic processes. Our intent is to provide an up-to-date review of the literature in the setting of head and neck diseases as well as describing our own initial results and areas of future research. Recent findingsWith increasing experience using CLE in the upper aerodigestive tract (UADT), evidence is mounting that this method can be a useful adjunct to standard endoscopy and other diagnostic techniques. Recent publications have shown that by using CLE, microanatomical structures of healthy and diseased mucosa can easily be identified, allowing for a differentiation of dysplastic/neoplastic and benign mucosal lesions. Standardized diagnostic protocols as well as clinically relevant classification systems for the UADT have not yet been described. SummaryCLE is an imaging modality that allows real-time visualization of mucosal cellular architecture and other histologic characteristics. First reports on its use in the UADT have yielded promising results, but the true value of this method is yet to be determined.

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Functional Imaging of Colonic Mucosa With a Fibered Confocal Microscope for Real-Time In Vivo Pathology

Abstract: Confocal imaging can be performed in vivo to assess the functional behavior of tissue in real time for providing pathologic interpretation, representing a new method for histologic evaluation.

Cited by 109 publications

References 27 publications

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Comprehensive spectral endoscopy of topically applied SERS nanoparticles in the rat esophagus

Confocal laser endomicroscopy in head and neck cancer

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