Functional implications of mitochondrial reactive oxygen species generated by oncogenic viruses

Choi, Young Bong; Harhaj, Edward W.

doi:10.1007/s11515-014-1332-0

Cited by 11 publications

(9 citation statements)

References 160 publications

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“…We examined the cellular energy metabolism with a focus on mitochondria and glycolysis. Virus infection is known to impair mitochondrial function in host cells [25,38,39]. In our study, the OCR and expression of UCP1 in IAV cells were lower than those in the control cells (Fig.…”

Section: Discussionmentioning

confidence: 51%

“…Therefore, mitochondria and glycolysis play important roles in cellular energy metabolism. Recent studies have revealed that mitochondria are responsible for regulating the mammalian innate immune response [20,21], particularly with regard to infection with RNA viruses (including influenza virus) [22,23,24,25,26,27]. Further, the effect of viruses on glycolytic metabolism has also been reported [28,29,30,31,32,33].…”

Section: Introductionmentioning

confidence: 99%

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Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection

Takanashi

Dan²,

Kanzaki³

et al. 2017

Pharmacology

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Background: Hochuekkito (HKT), a traditional Japanese herbal medicine (Kampo), has been used to treat symptoms of several diseases. In a recent clinical study, HKT was shown to be protective against the influenza virus infection. However, the underlying mechanism of the prophylactic effect is not clear. Mitochondrial and glycolytic pathways play important roles in cellular energy metabolism to maintain biological functions. These metabolic pathways are affected by the influenza virus infection. In this study, we examined the relationship between the preventive effects of HKT against the influenza virus infection and cellular energy metabolism in mitochondria and glycolysis using Madin-Darby canine kidney cells and influenza A/PR/8/34 (H1N1) virus (IAV). Methods: Mitochondrial and glycolytic metabolic pathways were evaluated on the basis of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), respectively, using the XF24 Extracellular Analyzer. Results: The OCR/ECAR ratio in IAV-infected cells was lower than that in control cells. Cells that were treated with HKT before IAV infection showed a metabolic pattern similar to that in the control cells (increase in both OCR and ECAR). Conclusions: Our results suggest that HKT not only activates both mitochondrial and glycolytic energy metabolism in IAV-infected cells but also helps maintain metabolic homeostasis similar to that in noninfected cells.

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Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection

Takanashi

Dan²,

Kanzaki³

et al. 2017

Pharmacology

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“…High-risk HPV infection may cause a series of mitochondrial dysfunction by accelerating the production of ROS [35,36]. Warowicka et al observed that both mtCN and ROS were increased during cervical cancer development [28].…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women

et al. 2020

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Background: Although human papillomavirus (HPV) infection has been regarded as the cause of cervical cancer in over 99% of cases, only a small fraction of HPV-infected women develop this malignancy. Emerging evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains undetermined. Methods: The current study included 591 cervical cancer cases and 373 cancer-free controls, all of whom were infected with high-risk HPV. Relative mtCN in cervical cancer exfoliated cells was measured by qRT-PCR assays, and logistic regression analysis was performed to compute odds ratios (ORs) and 95% confidence intervals (CIs). Interaction between mtCN and HPV types was assessed by using the Wald test in logistic regression models. Results: HPV16, 18, 52, and 58 were the most common types in both case and control groups. Median mtCN in cases was significantly higher than that in controls (1.63 vs. 1.23, P = 0.03). After adjustment for age and HPV types, the highest quartile of mtCN was associated with increased odds of having cervical cancer (OR = 1.77, 95% CI = 1.19, 2.62; P < 0.01), as compared to the lowest quartile. A dose-response effect of mtCN on cervical cancer was also observed (P trend < 0.001). The interaction between mtCN and HPV types was statistically nonsignificant. Conclusions: In women who test HPV positive, the increase of mtCN in cervical exfoliated cells is associated with cervical cancer. This suggests a potential role of mtCN in cervical carcinogenesis.

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“…High-risk HPV infection may cause a series of mitochondrial dysfunction by accelerating the production of ROS [34,35]. Warowicka et al observed that both mtCN and ROS were increased during cervical cancer development [29].…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women

Sun

Qin

Zhou

et al. 2019

Preprint

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Abstract Background: Although human papillomavirus (HPV) infection has been recognized as the major cause of cervical cancer, only a minority of HPV-infected women develop this malignancy. An increasing amount of evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains unknown. Methods: In this case-control study, we included 591 cervical cancer cases and 373 cancer-free controls, all of whom were infected with high-risk HPV. Relative mtCN in cervical cancer exfoliated cells was measured by qRT-PCR assays, and logistic regression analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs). Results: HPV16, 18, 52, and 58 were the most common types in both case and control groups. Median mtCN in cases was significantly higher than that in controls ( P = 0.03). After adjustment for age and HPV types, the highest quartile of mtCN was associated with increased odds of having cervical cancer (OR = 1.77, 95% CI = 1.19, 2.62; P < 0.01), as compared to the lowest quartile. A dose-response effect of mtCN on cervical cancer was also observed ( P trend < 0.001). The interaction between mtCN and HPV types was statistically nonsignificant. Conclusions: Increased mtCN in cervical exfoliated cells is associated with cervical cancer after HPV infection. Our study provides a basis for future studies to determine the potential of mtCN as a novel biomarker in cervical cancer screening.

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Functional implications of mitochondrial reactive oxygen species generated by oncogenic viruses

Cited by 11 publications

References 160 publications

Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection

Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection

Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women

Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women

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