Functional implications of quasi-equivalence in a T=3 icosahedral animal virus established by cryo-electron microscopy and X-ray crystallography

Cheng, R. Holland; Reddy, Vijay; Olson, Norman H.; Fisher, A.J.; Baker, Timothy S.; Johnson, John E.

doi:10.1016/s0969-2126(00)00029-0

Cited by 124 publications

(3 citation statements)

References 30 publications

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“…Single-particle image processing was carried out in Relion, which allowed us to determine the solution structure of the VLP to a global resolution of 3.7 Å (Supplementary Figure S1B). The ACNNV VLP is an isometric particle with T = 3 symmetry (Figures 4A,B), consistent with previously reported structures of the grouper nervous necrosis virus (GNNV) VLPs which share ∼85 % sequence identity (Cheng et al, 1994; Chen et al, 2015). The shell domain displays the highest local resolution (Supplementary Figure S1C) which allowed the building of an atomic model of the capsid shell (Figures 4C,D).…”

Section: Resultssupporting

confidence: 90%

“…RNA2 (1.4 kb) encodes the coat protein precursor alpha (43 kDa) and has an additional function in regulating the synthesis of RNA3 (Dasgupta et al, 1984; Friesen and Rueckert, 1984; Mori et al, 1992). The coat protein precursor self-assembles into T = 3 provirions (Friesen and Rueckert, 1981; Gallagher and Rueckert, 1988; Cheng et al, 1994). Provirions mature by spontaneous autocatalytic cleavage of the coat protein alpha post-assembly, producing proteins beta (38 kDa) and gamma (5 kDa) which remain part of the mature virion (Hosur et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

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Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

et al. 2019

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Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass ( Dicentrarchus labrax ) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

et al. 2019

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“…The coat protein precursor can self-assemble into provirions ( Gallagher and Rueckert, 1988 ; Cheng et al., 1994 ). NNV VLPs have the potential to be used for vaccine development.…”

Section: Plant-made Vaccines Against Viral Diseases In Farm Animalsmentioning

confidence: 99%

Plant-made vaccines against viral diseases in humans and farm animals

Eerde

Rimstad

et al. 2023

Front. Plant Sci.

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Plants provide not only food and feed, but also herbal medicines and various raw materials for industry. Moreover, plants can be green factories producing high value bioproducts such as biopharmaceuticals and vaccines. Advantages of plant-based production platforms include easy scale-up, cost effectiveness, and high safety as plants are not hosts for human and animal pathogens. Plant cells perform many post-translational modifications that are present in humans and animals and can be essential for biological activity of produced recombinant proteins. Stimulated by progress in plant transformation technologies, substantial efforts have been made in both the public and the private sectors to develop plant-based vaccine production platforms. Recent promising examples include plant-made vaccines against COVID-19 and Ebola. The COVIFENZ® COVID-19 vaccine produced in Nicotiana benthamiana has been approved in Canada, and several plant-made influenza vaccines have undergone clinical trials. In this review, we discuss the status of vaccine production in plants and the state of the art in downstream processing according to good manufacturing practice (GMP). We discuss different production approaches, including stable transgenic plants and transient expression technologies, and review selected applications in the area of human and veterinary vaccines. We also highlight specific challenges associated with viral vaccine production for different target organisms, including lower vertebrates (e.g., farmed fish), and discuss future perspectives for the field.

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Image processing approaches to biological three-dimensional electron microscopy

Herman

Marabini

Carazo

et al. 2000

Int. J. Imaging Syst. Technol.

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Electron microscopy is a powerful technique for imaging complex biological macromolecules in order to further the understanding of their functions. When combined with sufficiently careful sample preparation procedures that preserve the native structure of the macromolecules and with sophisticated image processing procedures, electron microscopy can lead to very informative estimates of the three‐dimensional (3D) structures of the specimens under study. 3D reconstruction from electron microscopic data is achieving high goals and exceeding expectations unthinkable only a few years ago. However, there are still some areas where either not enough work has been invested or the work has not as yet been fruitful. We describe image processing approaches that shed further light on some of these difficult areas. © 2000 John Wiley & Sons, Inc. Int J Imaging Syst Technol, 11, 12–29, 2000

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Functional implications of quasi-equivalence in a T=3 icosahedral animal virus established by cryo-electron microscopy and X-ray crystallography

Cited by 124 publications

References 30 publications

Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

Plant-made vaccines against viral diseases in humans and farm animals

Image processing approaches to biological three-dimensional electron microscopy

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