Functional improvement following implantation of a microstructured, type-I collagen scaffold into experimental injuries of the adult rat spinal cord

Altinova, Haktan; Möllers, Sven; Führmann, Tobias; Deumens, Ronald; Bozkurt, Ahmet; Heschel, I.; damink, L.H.H. Olde; Schügner, Frank; Weis, Joachim; Brook, Gary

doi:10.1016/j.brainres.2014.08.041

Cited by 29 publications

(34 citation statements)

References 80 publications

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“…Thus, a collagen scaffold (CS) was used to bridge the transected nerve stumps and sustain nerve regeneration. The results of previous studies showed that collagen scaffold implantation into the SCI site could promote nerve cell survival and neurite growth . Furthermore, this scaffold type promoted nerve regeneration and functional recovery .…”

Section: Discussionmentioning

confidence: 97%

Increased vascularization promotes functional recovery in the transected spinal cord rats by implanted vascular endothelial growth factor‐targeting collagen scaffold

Wang

Shi

et al. 2017

Journal Orthopaedic Research

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Spinal cord injury (SCI) is global health concern. The effective strategies for SCI are relevant to the improvement on nerve regeneration microenvironment. Vascular endothelial growth factor (VEGF) is an important cytokine for inducing angiogenesis and accelerating nerve system function recovery from injury. We proposed that VEGF could improve nerve regeneration in SCI. However, an uncontrolled delivery system target to injury site not only decreases the therapeutic efficacy but also increases the risk of tumor information. We implanted collagen scaffold (CS) targeted with a constructed protein, collagen-binding VEGF (CBD-VEGF), to bridge transected spine cord gap in a rat transected SCI model. Functional and histological examinations were conducted to assess the repair capacity of the delivery system CS/CBD-VEGF. The results indicated that the implantation of CS/CBD-VEGF into the model rats improved the survival rate and exerted beneficial effect on functional recovery. The controlled intervention improved the microenvironment, guided axon growth, and promoted neovascularization at the injury site. Therefore, the delivery system with stable binding of VEGF potentially provides a better therapeutic option for SCI. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1024-1034, 2018.

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Section: Discussionmentioning

confidence: 97%

Increased vascularization promotes functional recovery in the transected spinal cord rats by implanted vascular endothelial growth factor‐targeting collagen scaffold

Wang

Shi

et al. 2017

Journal Orthopaedic Research

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“…[325263052] In one study, completely transected spinal cord stumps of rats were bridged by collagen tubes. Results showed aligned axon growth within the tube lumen and a reduction in the density of glial scar formation.…”

Section: Discussionmentioning

confidence: 99%

“…At this early phase of our investigation of graphene as an adequate scaffold in the setting of SCI, we are not ready to compare our nanoscaffold with that of other authors. [341415171823252629303233363951525961] The novelty of our study lies in the use of graphene in an in vivo model of SCI. To the best of our knowledge, the biocompatibility and use of reduced graphene has not been investigated previously.…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of reduced graphene oxide nanoscaffolds following acute spinal cord injury in rats

et al. 2016

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Background:Graphene has unique electrical, physical, and chemical properties that may have great potential as a bioscaffold for neuronal regeneration after spinal cord injury. These nanoscaffolds have previously been shown to be biocompatible in vitro; in the present study, we wished to evaluate its biocompatibility in an in vivo spinal cord injury model.Methods:Graphene nanoscaffolds were prepared by the mild chemical reduction of graphene oxide. Twenty Wistar rats (19 male and 1 female) underwent hemispinal cord transection at approximately the T2 level. To bridge the lesion, graphene nanoscaffolds with a hydrogel were implanted immediately after spinal cord transection. Control animals were treated with hydrogel matrix alone. Histologic evaluation was performed 3 months after the spinal cord transection to assess in vivo biocompatibility of graphene and to measure the ingrowth of tissue elements adjacent to the graphene nanoscaffold.Results:The graphene nanoscaffolds adhered well to the spinal cord tissue. There was no area of pseudocyst around the scaffolds suggestive of cytotoxicity. Instead, histological evaluation showed an ingrowth of connective tissue elements, blood vessels, neurofilaments, and Schwann cells around the graphene nanoscaffolds.Conclusions:Graphene is a nanomaterial that is biocompatible with neurons and may have significant biomedical application. It may provide a scaffold for the ingrowth of regenerating axons after spinal cord injury.

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“…Collagen can connect the rostral and caudal segments of severely injured spinal cords, provide a 3D culture condition, and construct demanding microenvironments for the regulation of the fate of stem cells, direct nerve regrowth, and serve as a delivery vehicle for trophic factors, for exogenic and endogenic stem cells. Collagen, further bound with growth factors, stem cells, or other bioactive drugs, can promote the retention and neuronal differentiation of implanted NSCs, and improve the motor functions of SCI rats . According to the latest report, an open clinical study on the function of collagen‐based neuromaix was performed on 20 patients of sural nerve biopsy defect, and the clinical data first provide an evidence for the bridging function of collagen‐based neuromaix on larger nerve gaps in combined nerves .…”

Section: Classes Of Nanomaterials For the Imaging And Treatment Of Nementioning

confidence: 99%

Nanomaterials in Neural‐Stem‐Cell‐Mediated Regenerative Medicine: Imaging and Treatment of Neurological Diseases

et al. 2018

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Patients are increasingly being diagnosed with neuropathic diseases, but are rarely cured because of the loss of neurons in damaged tissues. This situation creates an urgent clinical need to develop alternative treatment strategies for effective repair and regeneration of injured or diseased tissues. Neural stem cells (NSCs), highly pluripotent cells with the ability of self-renewal and potential for multidirectional differentiation, provide a promising solution to meet this demand. However, some serious challenges remaining to be addressed are the regulation of implanted NSCs, tracking their fate, monitoring their interaction with and responsiveness to the tissue environment, and evaluating their treatment efficacy. Nanomaterials have been envisioned as innovative components to further empower the field of NSC-based regenerative medicine, because their unique physicochemical characteristics provide unparalleled solutions to the imaging and treatment of diseases. By building on the advantages of nanomaterials, tremendous efforts have been devoted to facilitate research into the clinical translation of NSC-based therapy. Here, recent work on emerging nanomaterials is highlighted and their performance in the imaging and treatment of neurological diseases is evaluated, comparing the strengths and weaknesses of various imaging modalities currently used. The underlying mechanisms of therapeutic efficacy are discussed, and future research directions are suggested.

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Functional improvement following implantation of a microstructured, type-I collagen scaffold into experimental injuries of the adult rat spinal cord

Cited by 29 publications

References 80 publications

Increased vascularization promotes functional recovery in the transected spinal cord rats by implanted vascular endothelial growth factor‐targeting collagen scaffold

Increased vascularization promotes functional recovery in the transected spinal cord rats by implanted vascular endothelial growth factor‐targeting collagen scaffold

Biocompatibility of reduced graphene oxide nanoscaffolds following acute spinal cord injury in rats

Nanomaterials in Neural‐Stem‐Cell‐Mediated Regenerative Medicine: Imaging and Treatment of Neurological Diseases

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