Functional insight into the glycosomal peroxiredoxin of Leishmania

Castro, Helena Carla; Rocha, Maria Inês; Silva, Ricardo; Oliveira, Filipe S.; Gomes‐Alves, Ana G.; Cruz, Tânia; Duarte, Margarida; Tomás, Ana M.

doi:10.1016/j.actatropica.2019.105217

Cited by 7 publications

(9 citation statements)

References 45 publications

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“…Retention of cPRX2-coding genes is a generalized feature of all Leishmania species with complete sequence annotation of the cPRX loci ( L. aethiopica , L. braziliensis , L. donovani , L. enriettii , L. mexicana , L. panamensis , and L. tarentolae ). Interestingly, Leishmaniinae are also unique in harboring a glycosomal PRX (gPRX) isoform [ 47 ], whose coding sequence falls within the same genetic locus as cPRX1 and cPRX2 . Based on the highly repetitive character of cPRX loci , e.g.…”

Section: Resultsmentioning

confidence: 99%

“…The construct to target LicPRX1 was built from a pTEX- HYG R -based plasmid previously used to disrupt LimTXNPx [ 17 ], by sequential cloning of the LicPRX1 5’UTR (primers P11/P12) and 3’UTR (primers P13/P14) into Bam HI/ Eco RV and Kpn I sites, respectively. The DNA cassette to target LicPRX2 was assembled from the pGL-HYG R -(5′UTRLicPRX1)-LiDRP-(3′UTRLigPRX) [ 47 ], by sequentially cloning the LicPRX2 5’UTR (primers P15/P16) and the LicPRX2 3’UTR (primers P17/P18) into Hin dIII/ Xma I and Bam HI/ Bgl II restriction sites, respectively. Prior to transfection, integration cassettes were separated from plasmid backbones by digestion with Bam HI ( LicPRX1 cassette) or Hin dIII/ Bgl II ( LicPRX2 cassette).…”

Section: Methodsmentioning

confidence: 99%

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The cytosolic hyperoxidation-sensitive and -robust Leishmania peroxiredoxins cPRX1 and cPRX2 are both dispensable for parasite infectivity

Castro,

Rocha,

Duarte

et al. 2024

Redox Biology

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The cytosolic hyperoxidation-sensitive and -robust Leishmania peroxiredoxins cPRX1 and cPRX2 are both dispensable for parasite infectivity

Castro,

Rocha,

Duarte

et al. 2024

Redox Biology

Self Cite

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“…This protein family has been described in a wide variety of organisms and several biological functions have been reported for Leishmania parasites, such as virulence factor and protection against reactive oxygen and nitrogen species [88]. In this manner, they are directly associated with cell proliferation, senescence, apoptosis, and circadian rhythms [89]. These proteins have been described in the secretome of L. braziliensis and the antigenicity of tryparedoxin peroxidase has also been evaluated for both human and canine VL-diagnosis [90][91][92].…”

Section: Plos Onementioning

confidence: 99%

Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review

et al. 2021

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Immunological tests may represent valuable tools for the diagnosis of human tegumentary leishmaniasis (TL) due to their simple execution, less invasive nature and potential use as a point-of-care test. Indeed, several antigenic targets have been used with the aim of improving the restricted scenario for TL-diagnosis. We performed a worldwide systematic review to identify antigenic targets that have been evaluated for the main clinical forms of TL, such as cutaneous (CL) and mucosal (ML) leishmaniasis. Included were original studies evaluating the sensitivity and specificity of immunological tests for human-TL, CL and/or ML diagnosis using purified or recombinant proteins, synthetic peptides or polyclonal or monoclonal antibodies to detect Leishmania-specific antibodies or antigens. The review methodology followed PRISMA guidelines and all selected studies were evaluated in accordance with QUADAS-2. Thirty-eight original studies from four databases fulfilled the selection criteria. A total of 79 antigens were evaluated for the detection of antibodies as a diagnostic for TL, CL and/or ML by ELISA. Furthermore, three antibodies were evaluated for the detection of antigen by immunochromatographic test (ICT) and immunohistochemistry (IHC) for CL-diagnosis. Several antigenic targets showed 100% of sensitivity and specificity, suggesting potential use for TL-diagnosis in its different clinical manifestations. However, a high number of proof-of-concept studies reinforce the need for further analysis aimed at verifying true diagnostic accuracy in clinical practice.

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“…Previous work identified the loss of peroxisomes in other free living related species 51 . Members of the peroxisomal proteins are studied as a drug target for the development of novel therapies against diseases caused by parasites [52][53][54][55] . We propose that the peroxisomal protein set, absent specifically in metazoan parasites, can be considered as targets for drugs against human parasites.…”

Section: Trait Lossmentioning

confidence: 99%

Conservation motifs - a novel evolutionary-based classification of proteins

Beer

Sherill-Rofe

Unterman

et al. 2020

Preprint

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Cross-species protein conservation patterns, as directed by natural selection, are indicative of the interplay between protein function, protein-protein interaction and evolution. Since the beginning of the genomic era, proteins were characterized as either conserved or not conserved. This simple classification became archaic and cursory once data on protein orthologs became available for thousands of species.To enrich the language used to describe protein conservation patterns, and to understand their biological significance, we classified 20,294 human proteins against 1096 species. Analyses of the conservation patterns of human proteins in different eukaryotic clades yielded extremely variable and rich patterns that had never been characterized or studied before. Using mathematical classifications, we defined seven conservation motifs: Steps, Critical, Lately Developed, Plateau, Clade Loss, Trait Loss and Gain, which describe the evolution of human proteins.One type of motif, which we termed Gain, describes the human proteins that are highly conserved in a small number of organisms but are not found in most other species.Interestingly, this pattern predicts 73 possible instances of horizontal gene transfer in eukaryotes.Overall, our work offers novel terms for conservation patterns and defines a new language intended to classify proteins based on evolution, reveal aspects of protein evolution, and improve the understanding of protein functions.

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Functional insight into the glycosomal peroxiredoxin of Leishmania

Cited by 7 publications

References 45 publications

The cytosolic hyperoxidation-sensitive and -robust Leishmania peroxiredoxins cPRX1 and cPRX2 are both dispensable for parasite infectivity

The cytosolic hyperoxidation-sensitive and -robust Leishmania peroxiredoxins cPRX1 and cPRX2 are both dispensable for parasite infectivity

Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review

Conservation motifs - a novel evolutionary-based classification of proteins

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