Functional interaction of CCAAT/enhancer-binding-protein-α basic region mutants with E2F transcription factors and DNA

Kowenz‐Leutz, Elisabeth; Schuetz, A.; Liu, Qingbin; Knoblich, Maria; Heinemann, Udo; Leutz, Achim

doi:10.1016/j.bbagrm.2016.04.008

Cited by 1 publication

(1 citation statement)

References 46 publications

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“…To further confirm, we generated a C/EBPα basic domain mutation (BRM2) construct (Fig. 2a) which proved to reduce the DNA binding ability (Kowenz-Leutz et al, 2016). However we observed strong RNA binding activity in BRM2 mutation, further confirming that DNA binding activity and RNA binding activity of are separated in C/EBPα.…”

Section: C/ebpα Interacts With Rna Through N Terminal Region But Not Bzip Domainmentioning

confidence: 62%

C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation

Zhang

Bassal

Friedrich

et al. 2021

Preprint

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C/EBPα has known to be a transcription factor that involved in Neutrophil differentiation for decades. However, exploring the Chromatin RNA Immunoprecipitation Sequencing (RIP), we discover that C/EBPα is a RNA binding protein mainly interacts with RNA introns. Structure study and RNA electrophoretic mobility shift assay (REMSA) show that C/EBPα interacts with RNA through two novel RNA binding domains distinct from its DNA binding domain. Mouse bone marrow transplantation and in vitro cytokine assay reveal that C/EBPα RNA binding is critical for Macrophage differentiation but not Neutrophil differentiation. Mechanically, RNA binding domains control specific gene transcription. In particular, PU.1 intron 4 RNA interacts with C/EBPα and recruit C/EBPα to its enhancer site, which facilitate PU.1 expression. Taken together, C/EBPα is demonstrated to be a RNA binding protein with unique function distinct from its DNA binding activity. Our finding transforms our knowledge of transcriptional regulation by transcription factor.

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Section: C/ebpα Interacts With Rna Through N Terminal Region But Not Bzip Domainmentioning

confidence: 62%

C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation

Zhang

Bassal

Friedrich

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Functional interaction of CCAAT/enhancer-binding-protein-α basic region mutants with E2F transcription factors and DNA

Abstract: bZip C/EBPα mutations and E2F

Cited by 1 publication

References 46 publications

C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation

C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation

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