Functional interactions between the oxytocin receptor and the β2-adrenergic receptor: Implications for ERK1/2 activation in human myometrial cells

Activation of β-adrenergic receptor promotes cellular proliferation in human glioblastoma

“…As aforementioned, obtaining β-AR expression in U87-MG tumors in vivo would imply that β-AR may facilitate tumor formation by promoting cellular proliferation. Another potential probability is that this cell type-specific divergence on cellular proliferation may be due to cross-talk between β-ARs and other GPCR-linked signaling cascades including gamma-aminobutyric acid B receptors (GABA B R) (37), α 2 -AR (38), bradykinin B 2 receptor (39), oxytocin (40), and cannabinoid receptors (28). For example, the ISO-induced signaling cascade, cellular proliferation and cellular migration in pancreatic ductal adenocarcinoma cells may be potently inhibited following stimulation of GABA B R signaling (37).…”

Gβ 4 γ 1 as a modulator of M3 muscarinic receptor signalling and novel roles of Gβ 1 subunits in the modulation of cellular signalling

“…An immunoblot-based assay to assess levels of ERK1/2 phosphorylation was performed as previously described [31,32]. Briefly, HEK 293 cells reverse transfected in 12-well plates were first serum-starved for 5 h at 37°C.…”

Advances in the role of oxytocin receptors in human parturition

“…Other studies have reported Src activation and β-arrestins to play a role in the stimulation of ERK1/2 [88,89]. Wrzal et al have recently demonstrated that OTR and β 2 -AR interact physiologically and that β 2 -AR requires interaction with OTR to activate ERK1/2 via PTX-sensitive, PKC-dependent pathway [90]. The fact that the ligands of OTR and β 2 -AR modulate the downstream signalling of either partner receptor in human myometrial cells provided further evidence for OTR-β 2 -AR crosstalk [76].…”