Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures

Fortunel, Nicolas O.; Vaigot, Pierre; Cadio, Emmanuelle; Martin, Michèle

doi:10.1007/978-1-60761-380-0_2

Cited by 10 publications

(4 citation statements)

References 13 publications

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“…Cells such as human dermal fibroblasts [27], foreskin derived keratinocytes [28], keratinocyte stem cells [29], hair follicle stem cells [30], angiogenic endothelial progenitor cells [31], bone marrow-derived mesenchymal stem cells [32], and adipose tissue derived mesenchymal stem cells [33] are mainly used for wound healing and tissue regeneration. Work is progressing toward co-culturing cells for tissue generation that involves keratinocytes and fibroblasts [34,35].…”

Section: Newer Approaches For Tissue Engineeringmentioning

confidence: 99%

Advances in Skin Regeneration Using Tissue Engineering

Vig

Chaudhari

Tripathi

et al. 2017

IJMS

534

418

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Tissue engineered skin substitutes for wound healing have evolved tremendously over the last couple of years. New advances have been made toward developing skin substitutes made up of artificial and natural materials. Engineered skin substitutes are developed from acellular materials or can be synthesized from autologous, allograft, xenogenic, or synthetic sources. Each of these engineered skin substitutes has their advantages and disadvantages. However, to this date, a complete functional skin substitute is not available, and research is continuing to develop a competent full thickness skin substitute product that can vascularize rapidly. There is also a need to redesign the currently available substitutes to make them user friendly, commercially affordable, and viable with longer shelf life. The present review focuses on providing an overview of advances in the field of tissue engineered skin substitute development, the availability of various types, and their application.

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Section: Newer Approaches For Tissue Engineeringmentioning

confidence: 99%

Advances in Skin Regeneration Using Tissue Engineering

Vig

Chaudhari

Tripathi

et al. 2017

IJMS

534

418

View full text Add to dashboard Cite

show abstract

“…The ability of polymers in skin regeneration is successfully evidenced by in vitro culture models using different types of skin cells. The most studied skin cells are human neonatal dermal fibroblasts, neonatal epidermal keratinocytes [153], HaCaT cells [154][155][156], epidermal pluripotent stem cells [157], foreskin-derived keratinocytes [158], dermal fibroblasts [159], keratinocyte stem cells [160], angiogenic endothelial progenitor cells [161], hair follicle stem cells [162], adipose-tissue-derived mesenchymal stem cells [163], and bone-marrow-derived mesenchymal stem cells [164].…”

Section: Types Of Cells Used As In Vitro Models For Skin Regenerationmentioning

confidence: 99%

Natural vs Synthetic Polymers: How Do They Communicate with Cells for Skin Regeneration—A Review

Elango,

Zamora-Ledezma,

Maté-Sánchez de Val

2023

J. Compos. Sci.

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Modern research has evolved several approaches toward skin regeneration and one of the novel concerns is the use of polymer-based systems due to their excellent beneficial properties to the skin. Several polymers, such as cellulose, hyaluronan, alginate, chitosan, collagen, fibrin and fibroin, have been tested and have proven the benefits for skin regeneration, and most of them are derived from either polysaccharide- or protein-based materials. In order to understand the mode of action, several researchers investigated the cell–matrix interaction and possible signaling mechanism in skin regeneration. Not only the signaling mechanism but also the mode of cell communication determines the application of polysaccharide- and protein-based polymers in practice. Based on the above significance, this review disclosed the recent findings to compile a possible method of communication between cells and polymers derived from polysaccharide-based (such as cellulose, hyaluronan, chitosan, alginate, agar, and xanthan gum) and protein-based (such as collagen, gelatin, fibrin, and silk fibroin) materials along with other polymers, such as poly(vinyl alcohol), polyglycolide or poly(glycolic acid), or poly(lactic acid) in skin regeneration. Accordingly, this review addresses the fundamental concept of cell–matrix communication, which helps us to understand the basis of the polymer’s functions in the biomedical field.

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“…Cell-based approaches to develop skin substitutes can involve differentiated cells or stem cells (adult, embryonic or induce pluripotent stem cells). Several different types of cells have been studied for wound healing in both preclinical and clinical settings such as human dermal fibroblasts [31], foreskin derived-keratinocytes [32], keratinocyte stem cells (KSCs) [33], hair follicle stem cells (HFSCs) [34,35], angiogenic endothelial progenitor cells (EPCs) [36], bone marrow-derived mesenchymal stem cells (BM-MSCs) [3], and adipose tissue-derived mesenchymal stem cells (AT-MSCs) [37].…”

Section: Cell Sources For Development Of Skin Substitutesmentioning

confidence: 99%

Critical Issues in Successful Production of Skin Substitutes for Wound Healing

Naderi‐Meshkin¹,

Amirkhah²,

Heirani‐Tabasi³

et al. 2018

gnc

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Novel findings on fabrication techniques for bioactive materials, discovering further basic knowledge about wound healing process, and availability of stem cells as alternative candidate for differentiated cells have highly encouraged scientists for developing new bioengineered skin substitutes (BSS) that offer an effective remedy for a specific wound type. However, technical, clinical, legislative and economic reasons hamper wide-spread commercialization and clinical translation of BSS. Among the various types of strategies that target skin repair and regeneration, tissue engineering with stem cells is most promising route. Tissue engineering by cooperation of several disciplines forms a context on which the commercial development of BSS is possible to provide benefits for patients who currently have limited or no cure options. The principles of tissue engineering are to initiate cell cultures in vitro, grow them in monolayer or on porous scaffolds and transplant the composite into a patient with a specific wound indication in vivo. The potential for creating of custom-designed biomaterials and availability of stem cells from either autologous or allogenic sources have helped to produce novel innovative BSS. Currently, wide range of skin substitutes are already being fabricated for clinical use in different wound indications but not yet definitively established. Therefore, many novel engineered constructs might be fabricated in the future. In this review, we describe the progress that has been made to date in the field of skin substitutes and the critical issues that are still hindering successful production and bench to bedside translation of BSS and restricting the availability of these innovative therapeutic constructs. Integrity of the science and technology, interdisciplinary expertise collaborations, and early interaction with regulatory entities such as Food and Drug Administration (FDA) and European Medicines Agency (EMA), together with other critical determinants, is vital to the successful commercialization of tissue engineering products into the marketplace/clinic.

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Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures

Cited by 10 publications

References 13 publications

Advances in Skin Regeneration Using Tissue Engineering

Advances in Skin Regeneration Using Tissue Engineering

Natural vs Synthetic Polymers: How Do They Communicate with Cells for Skin Regeneration—A Review

Critical Issues in Successful Production of Skin Substitutes for Wound Healing

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