Functional links between Drosophila Nipped-B and cohesin in somatic and meiotic cells

Gause, Maria; Webber, Hayley; Misulovin, Ziva; Haller, Gabe; Rollins, Robert A.; Eissenberg, Joel C.; Bickel, Sharon E.; Dorsett, Dale

doi:10.1007/s00412-007-0125-5

Cited by 64 publications

(93 citation statements)

References 74 publications

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“…Thus, the studies reported in this paper provide no information regarding the binding of cohesin or Nipped-B binding to centromeres. By immunostaining, cohesin binds to both mitotic and meiotic centromeres in Drosophila (Warren et al 2000;Valdeolmillos et al 2004;Khetani and Bickel 2007;Gause et al 2007). Immunostaining with the same Nipped-B antibody used in the experiments reported in this paper indicates that Nipped-B colocalizes with cohesin along chromosome arms in both polytene and meiotic chromosomes, but not at centromeres in meiotic chromosomes (Gause et al 2007).…”

Section: Discussion Cohesin Localization Differs Between Yeast and Drmentioning

confidence: 62%

“…Nipped-B, SA, and Smc1 antisera are described elsewhere (Dorsett et al 2005;Gause et al 2007). RNA polymerase II (PolII) antibody was purchased from Babco (MMS-126R).…”

Section: Methodsmentioning

confidence: 99%

“…These results do not mean, however, that repetitive sequences are generally cohesin-poor. By immunostaining, cohesin binds the pericentric heterochromatin of both mitotic and meiotic chromosomes in Drosophila (Warren et al 2000;Valdeolmillos et al 2004;Khetani and Bickel 2007;Gause et al 2007). …”

Section: Nipped-b and Cohesin Colocalize Genome-widementioning

confidence: 99%

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Association of cohesin and Nipped-B with transcriptionally active regions of the Drosophila melanogaster genome

et al. 2007

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Section: Discussion Cohesin Localization Differs Between Yeast and Drmentioning

confidence: 62%

“…Nipped-B, SA, and Smc1 antisera are described elsewhere (Dorsett et al 2005;Gause et al 2007). RNA polymerase II (PolII) antibody was purchased from Babco (MMS-126R).…”

Section: Methodsmentioning

confidence: 99%

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Association of cohesin and Nipped-B with transcriptionally active regions of the Drosophila melanogaster genome

et al. 2007

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“…Four of these complementation groups were identified molecularly in this study (see below). The Nipped-B complementation group was identified by the eight Nipped-B alleles sequenced by Gause et al (2008) (see Table 1). Similarly, Marygold et al (2005) previously sequenced line NC21 showing that it is an allele of RpL38.…”

Section: Resultsmentioning

confidence: 99%

Essential Loci in Centromeric Heterochromatin of Drosophila melanogaster. I: The Right Arm of Chromosome 2

et al. 2010

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With the most recent releases of the Drosophila melanogaster genome sequences, much of the previously absent heterochromatic sequences have now been annotated. We undertook an extensive genetic analysis of existing lethal mutations, as well as molecular mapping and sequence analysis (using a candidate gene approach) to identify as many essential genes as possible in the centromeric heterochromatin on the right arm of the second chromosome (2Rh) of D. melanogaster. We also utilized available RNA interference lines to knock down the expression of genes in 2Rh as another approach to identifying essential genes. In total, we verified the existence of eight novel essential loci in 2Rh: CG17665, CG17683, CG17684, CG17883, CG40127, CG41265, CG42595, and Atf6. Two of these essential loci, CG41265 and CG42595, are synonymous with the previously characterized loci l(2)41Ab and unextended, respectively. The genetic and molecular analysis of the previously reported locus, l(2)41Ae, revealed that this is not a single locus, but rather it is a large region of 2Rh that extends from unextended (CG42595) to CG17665 and includes four of the novel loci uncovered here.

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“…Cohesins copurify with Scc2/Scc4, suggesting that Scc2/Scc4 plays a direct role in deposition (8)(9)(10)(11). In the absence of either loader complex subunit, cohesin rings assemble, but fail to be deposited (7,12,13).…”

mentioning

confidence: 99%

Cell cycle-specific cleavage of Scc2 regulates its cohesin deposition activity

Woodman

Fara

Dzieciątkowska

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Sister chromatid cohesion (SCC), efficient DNA repair, and the regulation of some metazoan genes require the association of cohesins with chromosomes. Cohesins are deposited by a conserved heterodimeric loading complex composed of the Scc2 and Scc4 proteins in Saccharomyces cerevisiae, but how the Scc2/Scc4 deposition complex regulates the spatiotemporal association of cohesin with chromosomes is not understood. We examined Scc2 chromatin association during the cell division cycle and found that the affinity of Scc2 for chromatin increases biphasically during the cell cycle, increasing first transiently in late G 1 phase and then again later in G 2 /M. Inactivation of Scc2 following DNA replication reduces cellular viability, suggesting that this post S-phase increase in Scc2 chromatin binding affinity is biologically relevant. Interestingly, high and low Scc2 chromatin binding levels correlate strongly with the presence of full-length or amino-terminally cleaved forms of Scc2, respectively, and the appearance of the cleaved Scc2 species is promoted in vitro either by treatment with specific cell cycle-staged cellular extracts or by dephosphorylation. Importantly, Scc2 cleavage eliminates Scc2-Scc4 physical interactions, and an scc2 truncation mutant that mimics in vivo Scc2 cleavage is defective for cohesin deposition. These observations suggest a previously unidentified mechanism for the spatiotemporal regulation of cohesin association with chromosomes through cell cycle regulation of Scc2 cohesin deposition activity by Scc2 dephosphorylation and cleavage.M ultisubunit, ring-shaped cohesin complexes play key roles in chromosome morphogenesis that are required for faithful chromosome transmission to daughter cells. Newly replicated sister chromatids become tethered together by cohesins during S phase, which promotes chromosome biorientation on mitotic spindles (1). Cohesins also mediate efficient DNA double-strand break repair by homologous recombination (2, 3) and the formation or stabilization of chromatin loops that affect various nuclear processes, such as gene expression and Ig gene rearrangements (reviewed in refs. 4 and 5). Altered gene expression resulting from defective cohesinmediated chromatin looping is likely responsible for the pathogenesis of Cornelia de Lange Syndrome (CdLS), a dominantly inherited human developmental disorder (6).Sister chromatid cohesion (Scc) proteins form a heterodimeric cohesin deposition complex, but the complex's activity in deposition is not understood (7). Cohesins copurify with Scc2/Scc4, suggesting that Scc2/Scc4 plays a direct role in deposition (8-11). In the absence of either loader complex subunit, cohesin rings assemble, but fail to be deposited (7,12,13). ATP hydrolysis by cohesin's structural maintenance of chromosome (SMC) subunits is required for cohesin loading, and deposition is inhibited when SMC hinge domains, which mediate Smc1/3 interactions within cohesin, are artificially tethered (8,14,15). Thus, Scc2/ Scc4 may activate cohesin's ATPase activity or f...

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Functional links between Drosophila Nipped-B and cohesin in somatic and meiotic cells

Cited by 64 publications

References 74 publications

Association of cohesin and Nipped-B with transcriptionally active regions of the Drosophila melanogaster genome

Association of cohesin and Nipped-B with transcriptionally active regions of the Drosophila melanogaster genome

Essential Loci in Centromeric Heterochromatin of Drosophila melanogaster. I: The Right Arm of Chromosome 2

Cell cycle-specific cleavage of Scc2 regulates its cohesin deposition activity

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