Functional modulation of chemical mediators in microbial communities

Stallforth, Pierre; Mittag, Maria; Brakhage, Axel A.; Hertweck, Christian; Hellmich, Ute A.

doi:10.1016/j.tibs.2022.07.006

Cited by 13 publications

(4 citation statements)

References 71 publications

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“…Metagenomics involves sequencing the DNA or RNA directly from environmental samples, allowing researchers to study the collective genomes of microbial communities without the need for cultivation. Metatranscriptomics focuses on studying the gene expression patterns of microbial communities, providing insights into their functional activities and metabolic processes [33][34][35]. Metaproteomics involves analyzing the proteins produced by microbial communities and providing information about their metabolic activities and functional potential.…”

Section: Current Research and Technological Advancementsmentioning

confidence: 99%

Leveraging the Potential of Environmental Microorganisms: An Extensive Examination of Their Capacity in Tackling Global and Local Environmental and Ecological Challenges

Alabbosh

2024

Pol. J. Environ. Stud.

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The intricate web of environmental challenges faced by the global community necessitates a profound understanding of the potential solutions offered by nature's smallest inhabitantsenvironmental microorganisms. This comprehensive review paper seeks to illuminate the pivotal role of these microorganisms in addressing pressing environmental and ecological issues at both global and local scales. By investigating their many capacities and uses, this study hopes to shed light on the transformational influence that environmental microorganisms may have on attaining sustainability and resilience in the face of rising environmental issues. Through a detailed analysis of their adaptability, contributions to pollution control, agriculture, and ecosystem preservation, as well as the imperative need for further research and collaboration, this paper endeavors to underscore the profound significance of environmental microorganisms in shaping a harmonious coexistence between humanity and the natural world.

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Section: Current Research and Technological Advancementsmentioning

confidence: 99%

Leveraging the Potential of Environmental Microorganisms: An Extensive Examination of Their Capacity in Tackling Global and Local Environmental and Ecological Challenges

Alabbosh

2024

Pol. J. Environ. Stud.

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“…Furthermore, phenomena such as the instantly developing cobalt blue hue that occurs after bruising of e.g., a Cornflower Bolete (Gyroporus cyanescens) [2] contribute to an enigmatic aura associated with these mushrooms. However, the true relevance of terphenylquinones and their derivatives reaches far beyond a hue or a blueing reaction: Prokaryotic as well as eukaryotic members of microbial Seibold et al Fungal Biology and Biotechnology (2023) 10:14 consortia synthesize natural products to communicate and cooperate, or for defense [3][4][5][6][7]. In the case of the terphenylquinones, microbial consortia are impacted as these compounds inhibit bacterial motility and modulate biofilms [8].…”

Section: Introductionmentioning

confidence: 99%

Bifurcate evolution of quinone synthetases in basidiomycetes

Seibold

Lawrinowitz

Raztsou

et al. 2023

Fungal Biol Biotechnol

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Background The terphenylquinones represent an ecologically remarkable class of basidiomycete natural products as they serve as central precursors of pigments and compounds that impact on microbial consortia by modulating bacterial biofilms and motility. This study addressed the phylogenetic origin of the quinone synthetases that assemble the key terphenylquinones polyporic acid and atromentin. Results The activity of the Hapalopilus rutilans synthetases HapA1, HapA2 and of Psilocybe cubensis PpaA1 were reconstituted in Aspergilli. Liquid chromatography and mass spectrometry of the culture extracts identified all three enzymes as polyporic acid synthetases. PpaA1 is unique in that it features a C-terminal, yet catalytically inactive dioxygenase domain. Combined with bioinformatics to reconstruct the phylogeny, our results demonstrate that basidiomycete polyporic acid and atromentin synthetases evolved independently, although they share an identical catalytic mechanism and release structurally very closely related products. A targeted amino acid replacement in the substrate binding pocket of the adenylation domains resulted in bifunctional synthetases producing both polyporic acid and atromentin. Conclusions Our results imply that quinone synthetases evolved twice independently in basidiomycetes, depending on the aromatic α-keto acid substrate. Furthermore, key amino acid residues for substrate specificity were identified and changed which led to a relaxed substrate profile. Therefore, our work lays the foundation for future targeted enzyme engineering.

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“…Bacteria and fungi can both support the growth of microalgae, but they can also act antagonistically (Hom & Murray, 2014; Cooper & Smith, 2015; Aiyar et al ., 2017; Krespach et al ., 2020). Here, natural products play a major role in microbial interactions (reviewed in Stallforth, 2020; Stallforth et al ., 2023).…”

Section: Introductionmentioning

confidence: 99%

A cyclic lipopeptide produced by an antagonistic bacterium relies on its tail and transient receptor potential‐type Ca²⁺ channels to immobilize a green alga

et al. 2022

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Summary The antagonistic bacterium Pseudomonas protegens secretes the cyclic lipopeptide (CLiP) orfamide A, which triggers a Ca2+ signal causing rapid deflagellation of the microalga Chlamydomonas reinhardtii. We performed chemical synthesis of orfamide A derivatives and used an aequorin reporter line to measure their Ca2+ responses. Immobilization of algae was studied using a modulator and mutants of transient receptor potential (TRP)‐type channels. By investigating targeted synthetic orfamide A derivatives, we found that N‐terminal amino acids of the linear part and the terminal fatty acid region are important for the specificity of the Ca2+‐signal causing deflagellation. Molecular editing indicates that at least two distinct Ca2+‐signaling pathways are triggered. One is involved in deflagellation (Thr3 change, fatty acid tail shortened by 4C), whereas the other still causes an increase in cytosolic Ca2+ in the algal cells, but does not cause substantial deflagellation (Leu1 change, fatty acid hydroxylation, fatty acid changes by 2C). Using mutants, we define four TRP‐type channels that are involved in orfamide A signaling; only one (ADF1) responds additionally to low pH. These results suggest that the linear part of the CLiP plays one major role in Ca2+ signaling, and that orfamide A uses a network of algal TRP‐type channels for deflagellation.

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Functional modulation of chemical mediators in microbial communities

Cited by 13 publications

References 71 publications

Leveraging the Potential of Environmental Microorganisms: An Extensive Examination of Their Capacity in Tackling Global and Local Environmental and Ecological Challenges

Leveraging the Potential of Environmental Microorganisms: An Extensive Examination of Their Capacity in Tackling Global and Local Environmental and Ecological Challenges

Bifurcate evolution of quinone synthetases in basidiomycetes

A cyclic lipopeptide produced by an antagonistic bacterium relies on its tail and transient receptor potential‐type Ca²⁺ channels to immobilize a green alga

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Functional modulation of chemical mediators in microbial communities

Cited by 13 publications

References 71 publications

Leveraging the Potential of Environmental Microorganisms: An Extensive Examination of Their Capacity in Tackling Global and Local Environmental and Ecological Challenges

Leveraging the Potential of Environmental Microorganisms: An Extensive Examination of Their Capacity in Tackling Global and Local Environmental and Ecological Challenges

Bifurcate evolution of quinone synthetases in basidiomycetes

A cyclic lipopeptide produced by an antagonistic bacterium relies on its tail and transient receptor potential‐type Ca2+ channels to immobilize a green alga

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A cyclic lipopeptide produced by an antagonistic bacterium relies on its tail and transient receptor potential‐type Ca²⁺ channels to immobilize a green alga