Functional morphology of chorioallantoic vascular network in chicken

Максимов, В. Ф.; Korostyshevskaya, I. M.; Kurganov, S A

doi:10.1007/s10517-006-0368-9

Cited by 15 publications

(16 citation statements)

References 5 publications

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“…These experiments were performed as previously described (25,(35)(36)(37). Fertilized chicken eggs were incubated in a rotary incubator (37°C, 90% humidity) for 4 days, and then removed from the shell and placed in covered dishes (35).…”

Section: Quantitation Of Extravasation Efficiency Rates In Chicken Emmentioning

confidence: 99%

KISS1R Induces Invasiveness of Estrogen Receptor-Negative Human Mammary Epithelial and Breast Cancer Cells

et al. 2013

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Kisspeptins (KPs), peptide products of the KISS1 metastasis-suppressor gene, are endogenous ligands for a G protein-coupled receptor (KISS1R). KISS1 acts as a metastasis suppressor in numerous human cancers. However, recent studies have demonstrated that an increase in KISS1 and KISS1R expression in patient breast tumors correlates with higher tumor grade and metastatic potential. We have shown that KP-10 stimulates invasion of estrogen receptor ␣ (ER␣)-negative MDA-MB-231 breast cancer cells via transactivation of the epidermal growth factor receptor (EGFR). Here, we report that either KP-10 treatment of ER␣-negative nonmalignant mammary epithelial MCF10A cells or expression of KISS1R in MCF10A cells induced a mesenchymal phenotype and stimulated invasiveness. Similarly, exogenous expression of KISS1R in ER␣-negative SKBR3 breast cancer cells was sufficient to trigger invasion and induced extravasation in vivo. In contrast, KP-10 failed to transactivate EGFR or stimulate invasiveness in the ER␣-positive MCF7 and T47D breast cancer cells. This suggested that ER␣ negatively regulates KISS1R-dependent breast cancer cell migration, invasion, and EGFR transactivation. In support of this, we found that these KP-10-induced effects were ablated upon exogenous expression of ER␣ in the MDA-MB-231 cells, by down-regulating KISS1R expression. Lastly, we have identified IQGAP1, an actin cytoskeletal binding protein as a novel binding partner of KISS1R, and have shown that KISS1R regulates EGFR transactivation in breast cancer cells in an IQGAP1-dependent manner. Overall, our data strongly suggest that the ER␣ status of mammary cells dictates whether KISS1R may be a novel clinical target for treating breast cancer metastasis. (Endocrinology

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Section: Quantitation Of Extravasation Efficiency Rates In Chicken Emmentioning

confidence: 99%

KISS1R Induces Invasiveness of Estrogen Receptor-Negative Human Mammary Epithelial and Breast Cancer Cells

et al. 2013

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“…The most recent investigations have been addressed to clarifying mainly the CAM respiratory properties [22–24] and the multifunctional meaning of its structural patterns [25, 26]. In addition, studies in our laboratories have been performed and are still in progress, mainly aimed to elucidate the expression and significance of the CAM glycosylation patterns, as well as to identify and characterize membrane molecular components implicated in the ion transport processes which occur in the chorionic epithelium [27–31].…”

Section: Introductionmentioning

confidence: 99%

The Chick Chorioallantoic Membrane: A Model of Molecular, Structural, and Functional Adaptation to Transepithelial Ion Transport and Barrier Function during Embryonic Development

Gabrielli

Accili

2010

Journal of Biomedicine and Biotechnology

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The chick chorioallantoic membrane is a very simple extraembryonic membrane which serves multiple functions during embryo development; it is the site of exchange of respiratory gases, calcium transport from the eggshell, acid-base homeostasis in the embryo, and ion and H2O reabsorption from the allantoic fluid. All these functions are accomplished by its epithelia, the chorionic and the allantoic epithelium, by differentiation of a wide range of structural and molecular peculiarities which make them highly specialized, ion transporting epithelia. Studying the different aspects of such a developmental strategy emphasizes the functional potential of the epithelium and offers an excellent model system to gain insights into questions partly still unresolved.

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“…After five days of growth, Shield-1 or ethanol vehicle (in 4%EtOH, 100 µL/CAM) was injected intravenously [28], [30] to a final concentration of 1 or 2 µM Shield-1 as indicated. The average volume of a day 15 avian embryo is 8 mL[31]. Assuming Shield-1 will be uniformly distributed if injected intravenously, to establish a 1 µM final concentration of Shield-1, 100 µL of diluted Shield-1 (4 µL of 2 mM Shield-1 stock added to 96 µL PBS) was injected intravenously.…”

Section: Methodsmentioning

confidence: 99%

Imaging the Impact of Chemically Inducible Proteins on Cellular Dynamics In Vivo

Leong¹,

Lizardo

Ablack³

et al. 2012

PLoS ONE

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The analysis of dynamic events in the tumor microenvironment during cancer progression is limited by the complexity of current in vivo imaging models. This is coupled with an inability to rapidly modulate and visualize protein activity in real time and to understand the consequence of these perturbations in vivo. We developed an intravital imaging approach that allows the rapid induction and subsequent depletion of target protein levels within human cancer xenografts while assessing the impact on cell behavior and morphology in real time. A conditionally stabilized fluorescent E-cadherin chimera was expressed in metastatic breast cancer cells, and the impact of E-cadherin induction and depletion was visualized using real-time confocal microscopy in a xenograft avian embryo model. We demonstrate the assessment of protein localization, cell morphology and migration in cells undergoing epithelial-mesenchymal and mesenchymal-epithelial transitions in breast tumors. This technique allows for precise control over protein activity in vivo while permitting the temporal analysis of dynamic biophysical parameters.

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Functional morphology of chorioallantoic vascular network in chicken

Cited by 15 publications

References 5 publications

KISS1R Induces Invasiveness of Estrogen Receptor-Negative Human Mammary Epithelial and Breast Cancer Cells

KISS1R Induces Invasiveness of Estrogen Receptor-Negative Human Mammary Epithelial and Breast Cancer Cells

The Chick Chorioallantoic Membrane: A Model of Molecular, Structural, and Functional Adaptation to Transepithelial Ion Transport and Barrier Function during Embryonic Development

Imaging the Impact of Chemically Inducible Proteins on Cellular Dynamics In Vivo

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