Functional nucleic acid-based biosensors for virus detection

Zhang, Zijie; Adhikari, Bal Ram; Sen, Payel; Soleymani, Leyla; Li, Yingfu

doi:10.1016/j.aac.2023.07.006

Cited by 12 publications

(3 citation statements)

References 111 publications

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“…These viruses, with their distinct molecular structures, protein size ranges, and epidemic coexistence during seasonal outbreaks, provide a crucial basis for diversifying and specializing our nanosensor material developmental approaches (detailed specifications of seven target proteins are shown in Table S1). We targeted N proteins due to their high conservation compared to viral envelope proteins, making them a more reliable biomarker for the development of viral detection systems . NIR response of nanosensors to seven analytes was measured and calculated in heatmap (14 × 7 array) (Figure c).…”

Section: Resultsmentioning

confidence: 99%

“…18 We targeted N proteins due to their high conservation compared to viral envelope proteins, making them a more reliable biomarker for the development of viral detection systems. 19 NIR response of nanosensors to seven analytes was measured and calculated in heatmap (14 × 7 array) (Figure 2c). The signals were quantified as the difference in NIR fluorescence spectra before (I 0 ) and after virus N protein incubation (I) normalized by the initial fluorescence intensity: (I − I 0 )/I 0 .…”

Section: Design and Synthesis Of Peg-lipid-based 3d Coronamentioning

confidence: 99%

“…2,3 LFA offers simple, costeffectivene, and rapid results; however, its sensitivity and specificity can be less than optimal. 4 By experiencing a global health crisis from Coronavirus disease 2019 (COVID- 19), point-of-care (POC) diagnostics that overcome the limitations of PCR and LFI and facilitate rapid onsite testing are being rapidly developed. These emerging technologies are being developed to detect target RNA or viral proteins through transducer signal changes upon target molecular binding.…”

Section: Introductionmentioning

confidence: 99%

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Rational Design of 3D Polymer Corona Interfaces of Single-Walled Carbon Nanotubes for Receptor-Free Virus Recognition

Lee,

Kim,

Cho

et al. 2024

ACS Nano

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Facing the escalating threat of viruses worldwide, the development of efficient sensor elements for rapid virus detection has never been more critical. Traditional point-of-care (POC) sensors struggle due to their reliance on fragile biological receptors and limited adaptability to viral strains. In this study, we introduce a nanosensor design for receptor-free virus recognitions using near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) functionalized with a poly(ethylene glycol) (PEG)-phospholipid (PEG-lipid) array. Three-dimensional (3D) corona interfaces of the nanosensor array enable selective and sensitive detection of diverse viruses, including Ebola, Lassa, H3N2, H1N1, Middle East respiratory syndrome (MERS), severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1), and SARS-CoV-2, even without any biological receptors. The PEG-lipid components, designed considering chain length, fatty acid saturation, molecular weight, and endgroup moieties, allow for precise quantification of viral recognition abilities. High-throughput automated screening of the array demonstrates how the physicochemical properties of the PEG-lipid/SWCNT 3D corona interfaces correlate with viral detection efficiency. Utilizing molecular dynamics and AutoDock simulations, we investigated the impact of PEG-lipid components on 3D corona interface formation, such as surface coverage and hydrodynamic radius and specific molecular interactions based on chemical potentials. Our findings not only enhance detection specificity across various antigens but also accelerate the development of sensor materials for promptly identifying and responding to emerging antigen threats.

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Section: Resultsmentioning

confidence: 99%

Section: Design and Synthesis Of Peg-lipid-based 3d Coronamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rational Design of 3D Polymer Corona Interfaces of Single-Walled Carbon Nanotubes for Receptor-Free Virus Recognition

Lee,

Kim,

Cho

et al. 2024

ACS Nano

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Small‐Molecule Fluorescent Probes for Plant Hormones and their Receptors

Lin,

Xiong,

Zhou

et al. 2024

Analysis & Sensing

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Plant hormones and their receptors play a crucial role in regulating plant growth and adapting to the stress environment. The exploration of interaction between plant hormones and their receptors is significant to comprehend the molecular mechanisms of plant growth and development, the response mechanisms of adaptation to environmental changes, and to optimize the traits and stress‐resistance of crops. Since the biosynthesis, transport, and metabolism of hormones in plants are closely relevant to spatio‐temporal changes, and their content and distribution are highly dynamic, there is an urgent need for a qualitative and quantitative tool to accurately, real‐time, and in situ monitor the dynamic changes of hormones in plants without injury. Fluorescent probes have been widely used in the sensing and imaging of plant hormones and their receptors, due to their high spatio‐temporal resolution, high selectivity, non‐invasive, high sensitivity, and tailored molecular structures. Here, this paper provides a systematical overview of the research progress in the sensing and imaging of plant hormones and their receptors using fluorescent probes. In addition, the potential prospects and remaining challenges are also discussed to design fluorescent probes with better performance and promote the development of this field.

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