Functional Organization of Dorsal Horn Interneurons

Willis, William D.; Coggeshall, Richard E.

doi:10.1007/978-1-4615-0035-3_7

Cited by 6 publications

(1 citation statement)

References 4,059 publications

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“…We performed in vivo extracellular recording of wide-dynamicrange (WDR) neurons that make synaptic contacts with cuta-neous Ad-, C-, and Ab-fibers in spinal laminae III-V. These fibers respond to both thermal and mechanical stimuli (Willis and Coggeshall, 2004). Stimulation of the receptive field of WDR neurons on the plantar surface of the paw with natural thermal or mechanical stimuli (Urch and Dickenson, 2003) increased the AP firing (A) PCR genotyping showed bands that correspond to heterozygote, Fstl1 F/+ (399 and 505 bp), homozygote, Fstl1 F/F (505 bp only), and wild-type animals, Fstl1 +/+ (399 bp only).…”

Section: Neuronmentioning

confidence: 99%

Follistatin-like 1 Suppresses Sensory Afferent Transmission by Activating Na+,K+-ATPase

Zhang

et al. 2011

Neuron

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Excitatory synaptic transmission is modulated by inhibitory neurotransmitters and neuromodulators. We found that the synaptic transmission of somatic sensory afferents can be rapidly regulated by a presynaptically secreted protein, follistatin-like 1 (FSTL1), which serves as a direct activator of Na(+),K(+)-ATPase (NKA). The FSTL1 protein is highly expressed in small-diameter neurons of the dorsal root ganglion (DRG). It is transported to axon terminals via small translucent vesicles and secreted in both spontaneous and depolarization-induced manners. Biochemical assays showed that FSTL1 binds to the α1 subunit of NKA and elevates NKA activity. Extracellular FSTL1 induced membrane hyperpolarization in cultured cells and inhibited afferent synaptic transmission in spinal cord slices by activating NKA. Genetic deletion of FSTL1 in small DRG neurons of mice resulted in enhanced afferent synaptic transmission and sensory hypersensitivity, which could be reduced by intrathecally applied FSTL1 protein. Thus, FSTL1-dependent activation of NKA regulates the threshold of somatic sensation.

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Section: Neuronmentioning

confidence: 99%

Follistatin-like 1 Suppresses Sensory Afferent Transmission by Activating Na+,K+-ATPase

Zhang

et al. 2011

Neuron

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Medial thalamotomy using stereotactic radiosurgery for intractable pain: a systematic review

et al. 2021

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Neuronal calcium-binding proteins 1/2 localize to dorsal root ganglia and excitatory spinal neurons and are regulated by nerve injury

Zhang¹,

Tortoriello

Hsueh

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Neuronal calcium (Ca 2+ )-binding proteins 1 and 2 (NECAB1/2) are members of the phylogenetically conserved EF-hand Ca 2+ -binding protein superfamily. To date, NECABs have been explored only to a limited extent and, so far, not at all at the spinal level. Here, we describe the distribution, phenotype, and nerve injury-induced regulation of NECAB1/NECAB2 in mouse dorsal root ganglia (DRGs) and spinal cord. In DRGs, NECAB1/2 are expressed in around 70% of mainly small-and medium-sized neurons. Many colocalize with calcitonin gene-related peptide and isolectin B4, and thus represent nociceptors. NECAB1/2 neurons are much more abundant in DRGs than the Ca 2+ -binding proteins (parvalbumin, calbindin, calretinin, and secretagogin) studied to date. In the spinal cord, the NECAB1/2 distribution is mainly complementary. NECAB1 labels interneurons and a plexus of processes in superficial layers of the dorsal horn, commissural neurons in the intermediate area, and motor neurons in the ventral horn. Using CLARITY, a novel, bilaterally connected neuronal system with dendrites that embrace the dorsal columns like palisades is observed. NECAB2 is present in cell bodies and presynaptic boutons across the spinal cord. In the dorsal horn, most NECAB1/2 neurons are glutamatergic. Both NECAB1/2 are transported into dorsal roots and peripheral nerves. Peripheral nerve injury reduces NECAB2, but not NECAB1, expression in DRG neurons. Our study identifies NECAB1/2 as abundant Ca 2+ -binding proteins in painrelated DRG neurons and a variety of spinal systems, providing molecular markers for known and unknown neuron populations of mechanosensory and pain circuits in the spinal cord.commissural interneurons | GAD67 | neuropathic pain | VGLUT2

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Functional Organization of Dorsal Horn Interneurons

Cited by 6 publications

References 4,059 publications

Follistatin-like 1 Suppresses Sensory Afferent Transmission by Activating Na+,K+-ATPase

Follistatin-like 1 Suppresses Sensory Afferent Transmission by Activating Na+,K+-ATPase

Medial thalamotomy using stereotactic radiosurgery for intractable pain: a systematic review

Neuronal calcium-binding proteins 1/2 localize to dorsal root ganglia and excitatory spinal neurons and are regulated by nerve injury

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