Functional Properties of Hyalocytes under PDGF-Rich Conditions

Noda, Yoichi; Hata, Yoshinobu; Hisatomi, Toshio; Nakamura, Yuka; Hirayama, Kyosuke; Matsuda, Masayoshi; Nakao, Shintaro; Fujisawa, Kimihiko; Sakamoto, Taiji; Ishibashi, Tatsuro

doi:10.1167/iovs.03-1092

Cited by 44 publications

(47 citation statements)

References 33 publications

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“…Cell culture. Bovine hyalocytes were isolated as we previously reported (33). Cultured hyalocytes obtained between passages 5 and 7 were used in experiments.…”

Section: Methodsmentioning

confidence: 99%

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Potent Inhibition of Cicatricial Contraction in Proliferative Vitreoretinal Diseases by Statins

et al. 2008

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OBJECTIVE-Despite tremendous progress in vitreoretinal surgery, certain postsurgical complications limit the success in the treatment of proliferative vitreoretinal diseases (PVDs), such as proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR). One of the most significant complications is the cicatricial contraction of proliferative membranes, resulting in tractional retinal detachment and severe vision loss. Novel pharmaceutical approaches are thus urgently needed for the management of these vision-threatening diseases. In the current study, we investigated the inhibitory effects of statins on the progression of PVDs.RESEARCH DESIGN AND METHODS-Human vitreous concentrations of transforming growth factor-␤2 (TGF-␤2) were measured by enzyme-linked immunosorbent assay. TGF-␤2-and vitreous-dependent phosphorylation of myosin light chain (MLC), a downstream mediator of Rho-kinase pathway, and collagen gel contraction simulating cicatrical contraction were analyzed using cultured hyalocytes. Inhibitory effects of simvastatin on cicatrical contraction were assessed both in vitro and in vivo.RESULTS-Human vitreous concentrations of TGF-␤2 were significantly higher in the samples from patients with PVD compared with those without PVD. Simvastatin inhibited TGF-␤2-dependent MLC phosphorylation and gel contraction in a dose-and time-dependent manner and was capable of inhibiting translocation of Rho protein to the plasma membrane in the presence of TGF-␤2. Vitreous samples from patients with PVD enhanced MLC phosphorylation and gel contraction, whereas simvastatin almost completely inhibited these phenomena. Finally, intravitreal injection of simvastatin dose-dependently prevented the progression of diseased states in an in vivo model of PVR.CONCLUSIONS-Statins might have therapeutic potential in the prevention of PVDs.

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“…Cell culture. Bovine hyalocytes were isolated as we previously reported (33). Cultured hyalocytes obtained between passages 5 and 7 were used in experiments.…”

Section: Methodsmentioning

confidence: 99%

“…Collagen gel contraction assay. The contraction assay was performed as we previously described (33). Type I collagen (Koken, Tokyo), a reconstitution buffer, hyalocytes suspension, and distilled water were mixed and added to a 24-multiwell plate (Nunc, Roskilde, Denmark).…”

Section: Methodsmentioning

confidence: 99%

Potent Inhibition of Cicatricial Contraction in Proliferative Vitreoretinal Diseases by Statins

et al. 2008

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“…Morphological studies have demonstrated that hyalocytes, which possess lysosome-like granules, mitochondria, and micropinocytotic vesicles in their cytoplasm, belong to the monocyte/ macrophage lineage (22)(23)(24). They are considered to be originated from bone marrow and to regenerate in the vitreous cavity (25), as well as to be actively associated with the maintenance of vitreous transparency under physiological conditions (26,27).…”

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confidence: 99%

Transforming Growth Factor-β2 and Connective Tissue Growth Factor in Proliferative Vitreoretinal Diseases

et al. 2007

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The critical association of connective tissue growth factor (CTGF), which is thought to be one of the downstream mediators of transforming growth factor-␤ (TGF-␤), with vitreoretinal diseases remains to be clarified. In the current study, we first demonstrated the correlation between the concentrations of TGF-␤2 as well as CTGF in the vitreous and CTGF gene regulation in cultured hyalocytes. Concentrations of TGF-␤2 and CTGF in the vitreous

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“…Collagen gel contraction assay. The contraction assay was performed as we previously described (24). Hyalocytes and BRPEs were collected by the treatment of cultures with trypsin-EDTA for 3 min, washed with DMEM, and resuspended in DMEM at a density of 2.2 ϫ 10 6 cells/ml.…”

Section: Methodsmentioning

confidence: 99%

Functional Characteristics of Connective Tissue Growth Factor on Vitreoretinal Cells

et al. 2007

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Functional Properties of Hyalocytes under PDGF-Rich Conditions

Cited by 44 publications

References 33 publications

Potent Inhibition of Cicatricial Contraction in Proliferative Vitreoretinal Diseases by Statins

Potent Inhibition of Cicatricial Contraction in Proliferative Vitreoretinal Diseases by Statins

Transforming Growth Factor-β2 and Connective Tissue Growth Factor in Proliferative Vitreoretinal Diseases

Functional Characteristics of Connective Tissue Growth Factor on Vitreoretinal Cells

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