2007
DOI: 10.1128/jb.01452-06
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Functional Reconstitution of SdcS, a Na + -Coupled Dicarboxylate Carrier Protein from Staphylococcus aureus

Abstract: In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na ؉ -linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na ؉ symporter (DASS) family, a group that includes the mammalian Na ؉ /dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expressed SdcS in Escherichia coli and found it to have transport properties similar to those of its eukaryotic counterparts (J. A. Hall and A. M. Pajor, J. Bacteriol. 187:5189-5194, 2005). Here,… Show more

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Cited by 26 publications
(40 citation statements)
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“…This pattern is in contrast with succinate transport by the Staphylococcus aureus protein SdcS, a sodium-coupled C 4 -dicarboxylate transporter of the divalent anion:Na ϩ symporter (DASS) family, which has been characterized using proteoliposomes containing purified protein. SdcS transports a doubly deprotonated substrate together with two sodium ions, resulting in electroneutral transport (13). On the other hand, transport by eukaryotic homologues of SdcS (members of the SlC13 subfamily) is electrogenic; three sodium ions are cotransported with a divalent substrate (25).…”
Section: Discussionmentioning
confidence: 99%
“…This pattern is in contrast with succinate transport by the Staphylococcus aureus protein SdcS, a sodium-coupled C 4 -dicarboxylate transporter of the divalent anion:Na ϩ symporter (DASS) family, which has been characterized using proteoliposomes containing purified protein. SdcS transports a doubly deprotonated substrate together with two sodium ions, resulting in electroneutral transport (13). On the other hand, transport by eukaryotic homologues of SdcS (members of the SlC13 subfamily) is electrogenic; three sodium ions are cotransported with a divalent substrate (25).…”
Section: Discussionmentioning
confidence: 99%
“…Transporters of the TRAP family are three-component systems for secondary uptake of malate, fumarate, and succinate like the DctPQM system of Rhodobacter capsulatus (18). Another class of transporters for di-and tricarboxylates as well as amino acids or inorganic sulfate is represented by the DASS family, of which the SdcS transporter of Staphylococcus aureus has recently been characterized (23). Furthermore, the DAACS family of (putative) dicarboxylate transporters comprises transport systems for the uptake of TCA cycle intermediates and amino acids in symport with Na ϩ or H ϩ .…”
Section: Discussionmentioning
confidence: 99%
“…The first described member of the TRAP family is the C 4 -dicarboxylate transporter DctPQM from Rhodobacter capsulatus, which facilitates substrate uptake by the use of an extracytoplasmic solute receptor (8). An example of the DASS family, members of which occur in bacteria, as well in eukaryotes, is the well-characterized transporter SdcS from Staphylococcus aureus (13). Members of the CitHMS family import citrate in symport with the cation Mg 2ϩ or Ca 2ϩ .…”
mentioning
confidence: 99%