Functional regeneration of tendons using scaffolds with physical anisotropy engineered via microarchitectural manipulation

Wang, Z.; Lee, Won-Jae; Koh, Bryan T. H.; Hong, Minghui; Wang, W.; Lim, Poon Nian; Feng, Jinkui; Park, L. S.; Kim, M.; Thian, Eng San

doi:10.1126/sciadv.aat4537

Cited by 70 publications

(62 citation statements)

References 47 publications

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“…Although more critical in the MEW process, most of the spinning techniques face similar challenges with electrical charge dynamics that limit their scaling‐up perspectives. To overcome this, researchers have looked at hybrid methods that combine spinning with textile technologies . This approach typically converts mono‐ or multifibers, initially produced by SES, MEW, or wet spinning, into volumetric relevant structures by using different textile approaches like weaving, braiding, or knitting.…”

Section: Recent Advances In Techniques Controlling Fibrous Architecturementioning

confidence: 99%

“…Further in vitro analysis with human tenocytes demonstrated that the fibrous topography and alignment of the woven fabrics can guide cell alignment, organization, and type I collagen deposition. Wang et al further developed a different hybrid strategy to increase tensile mechanical properties ( Figure a) . The authors fabricated 3D fibrous scaffolds that comprised a core region of aligned SES PCL nanofibers, wrapped by a single‐shell film with elongated pores obtained by laser perforation.…”

Section: Recent Advances In Techniques Controlling Fibrous Architecturementioning

confidence: 99%

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Role of Biomaterials and Controlled Architecture on Tendon/Ligament Repair and Regeneration

et al. 2019

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Engineering synthetic scaffolds to repair and regenerate ruptured native tendon and ligament (T/L) tissues is a significant engineering challenge due to the need to satisfy both the unique biological and biomechanical properties of these tissues. Long‐term clinical outcomes of synthetic scaffolds relying solely on high uniaxial tensile strength are poor with high rates of implant rupture and synovitis. Ideal biomaterials for T/L repair and regeneration need to possess the appropriate biological and biomechanical properties necessary for the successful repair and regeneration of ruptured tendon and ligament tissues.

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Section: Recent Advances In Techniques Controlling Fibrous Architecturementioning

confidence: 99%

Section: Recent Advances In Techniques Controlling Fibrous Architecturementioning

confidence: 99%

Role of Biomaterials and Controlled Architecture on Tendon/Ligament Repair and Regeneration

et al. 2019

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“…The difficult for scalable fabrication and handling issues also relate to this method for the orientated fibre fabrication and future tissue-engineering application. A recent development to draw electrospun mats cyclically is reported to introduce orientated wave-like fibres, which show enlarged porosity capable of inducing cell infiltration and tissue ingrowth [54].…”

Section: Single-axial Drawing Of Polymersmentioning

confidence: 99%

“…larger than 85% of cells aligned in ±10° [42]), rapid cell reorientation [42], and a wide applicability for different cells (e.g. mesenchymal stem cells [42], endothelical cells [18,43] and tenocytes [54,55]). This method advances the fabrication of ridges and grooves on freestanding and flexible thin film over the whole surface, providing simple, solvent-free, and reproducible produce of cell alignment.…”

Section: Single-axial Drawing Of Polymersmentioning

confidence: 99%

“…To increase in vivo adaptation, vascular grafts are expected to maintain an appropriate phenotype of cells as in the native healthy blood vessels, and it is becoming clearer that geometric anisotropy acts a crucial role in regulating cell morphology and function for vascular tissue-engineering application. Mechanism behind these regulations is known as a mechano-transduction pathway that can be direct or indirect [18,54,71,89]. Direct mechanotransduction relies on the links between a cell shape and nucleus through the cytoskeleton and nuclear lamins.…”

Section: Cellular Differentiationmentioning

confidence: 99%

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Geometric anisotropy on biomaterials surface for vascular scaffold design: engineering and biological advances

et al. 2019

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Scaffold designs in combination with drug, growth factor and other bioactive chemicals account for lasting progress of vascular tissue engineering in the past decades. It is a great achievement to adjust tissue matrix composition and cell behaviour effectively. However, regenerating the innate physiologies of a blood vessel still needs its precise architecture to supply the vessel with structural basis for vascular functionality. Recent developments in biomaterial engineering have been explored in designing anisotropic surface geometries, and in turn to direct biological effects for recapitulating vascular tissue architecture. Here, we present current efforts, and propose future perspectives for the guidance on the architectural reconstruction and scaffold design of blood vessel.

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Electrospinning and Cell Fibers in Biomedical Applications

Zhao

Wang

2023

Advanced Biology

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Human body tissues such as muscle, blood vessels, tendon/ligaments, and nerves have fiber‐like fascicle morphologies, where ordered organization of cells and extracellular matrix (ECM) within the bundles in specific 3D manners orchestrates cells and ECM to provide tissue functions. Through engineering cell fibers (which are fibers containing living cells) as living building blocks with the help of emerging “bottom‐up” biomanufacturing technologies, it is now possible to reconstitute/recreate the fiber‐like fascicle morphologies and their spatiotemporally specific cell‐cell/cell‐ECM interactions in vitro, thereby enabling the modeling, therapy, or repair of these fibrous tissues. In this article, a concise review is provided of the “bottom‐up” biomanufacturing technologies and materials usable for fabricating cell fibers, with an emphasis on electrospinning that can effectively and efficiently produce thin cell fibers and with properly designed processes, 3D cell‐laden structures that mimic those of native fibrous tissues. The importance and applications of cell fibers as models, therapeutic platforms, or analogs/replacements for tissues for areas such as drug testing, cell therapy, and tissue engineering are highlighted. Challenges, in terms of biomimicry of high‐order hierarchical structures and complex dynamic cellular microenvironments of native tissues, as well as opportunities for cell fibers in a myriad of biomedical applications, are discussed.

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Functional regeneration of tendons using scaffolds with physical anisotropy engineered via microarchitectural manipulation

Abstract: Polymer axial drawing enables the design of optimal microarchitectured scaffolds for tendon regeneration.

Cited by 70 publications

References 47 publications

Role of Biomaterials and Controlled Architecture on Tendon/Ligament Repair and Regeneration

Role of Biomaterials and Controlled Architecture on Tendon/Ligament Repair and Regeneration

Geometric anisotropy on biomaterials surface for vascular scaffold design: engineering and biological advances

Electrospinning and Cell Fibers in Biomedical Applications

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