Functional role of 64P, the candidate transmission-blocking vaccine antigen from the tick, Rhipicephalus appendiculatus

Havlíková, Sabína; Roller, Ladislav; Koči, Juraj; Trimnell, Adama R.; Kazimírová, Mária; Klempa, Boris; Nuttall, Patricia A.

doi:10.1016/j.ijpara.2009.05.005

Cited by 37 publications

(42 citation statements)

References 38 publications

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“…The integration of omics data sets must overcome important challenges such as development of algorithms that will allow for analysis and validation of data produced by the systems biology approach to tick research and development of effective screening platforms for the selection of candidate protective antigens [26]. Systems biology studies for the selection of candidate protective antigens should focus on the characterization of physiological processes such as suppression of host immune responses, blood digestion, embryogenesis, innate immunity and tick-pathogen interactions that are critical for tick feeding, reproduction and vector capacity [26,56,66,[92][93][94][95][96][97][98][99][100][101][102][103][104][105].…”

Section: Discussionmentioning

confidence: 99%

Tick vaccines: current status and future directions

Fuente

Contreras

2015

Expert Review of Vaccines

111

117

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Ticks and tick-borne diseases are a growing problem affecting human and animal health worldwide. Traditional control methods, based primarily on chemical acaricides, have proven not to be sustainable because of the selection of acaricide-resistant ticks. Tick vaccines appear to be a promising and effective alternative for control of tick infestations and pathogen transmission. The purpose of this review is to summarize previous tick vaccine development and performance and formulate critical issues and recommendations for future directions for the development of improved and effective tick vaccines. The development of effective screening platforms and algorithms using omics approaches focused on relevant biological processes will allow the discovery of new tick-protective antigens. Future vaccines will likely combine tick antigens with different protective mechanisms alone or pathogen-derived antigens. The application of tick vaccines as a part of integrated control strategies will ultimately result in the control of tick-borne diseases.

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Section: Discussionmentioning

confidence: 99%

Tick vaccines: current status and future directions

Fuente

Contreras

2015

Expert Review of Vaccines

111

117

View full text Add to dashboard Cite

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“…The best solubilization can be achieved by using hot bases or acids (Kemp et al ., 1982), which, however, makes proteomic analysis almost impossible because of hydrolysis. Several proteins are known to be present in tick cement: examples are a 94 kDa protein detected from various tick species, a 20 kDa protein of Amblyomma americanum and a 15 kDa protein of Rhipicephalus appendiculatus , named 64P (Brown, Shapiro & Askenase, 1984; Shapiro, Voigt & Fujisaki, 1986; Shapiro, Voigt & Ellis, 1989; Havlikova et al ., 2009). …”

Section: Biochemistry Of Cementmentioning

confidence: 99%

“…The identification of nucleotide sequences which encode potential GRPs from cDNA libraries of three different tick species [ Amblyomma cajennense (Fabricius, 1787), Rhipicephalus microplus , R. sanguineus ] seems to confirm these suggestions (Maruyama et al ., 2010). However, GRPs may have other functions: they are also found in antimicrobial substances and are related to immune evasion (Francischetti et al ., 2009; Havlikova et al ., 2009). Proteins of this class are also found in the family Argasidae (Maruyama et al ., 2010), which do not produce cement.…”

Section: Biochemistry Of Cementmentioning

confidence: 99%

Tick attachment cement – reviewing the mysteries of a biological skin plug system

et al. 2017

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The majority of ticks in the family Ixodidae secrete a substance anchoring their mouthparts to the host skin. This substance is termed cement. It has adhesive properties and seals the lesion during feeding. The particular chemical composition and the curing process of the cement are unclear. This review summarizes the literature, starting with a historical overview, briefly introducing the different hypotheses on the origin of the adhesive and how the tick salivary glands have been identified as its source. Details on the sequence of cement deposition, the curing process and detachment are provided. Other possible functions of the cement, such as protection from the host immune system and antimicrobial properties, are presented. Histochemical and ultrastructural data of the intracellular granules in the salivary gland cells, as well as the secreted cement, suggest that proteins constitute the main material, with biochemical data revealing glycine to be the dominant amino acid. Applied methods and their restrictions are discussed. Tick cement is compared with adhesives of other animals such as barnacles, mussels and sea urchins. Finally, we address the potential of tick cement for the field of biomaterial research and in particular for medical applications in future.

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“…The protective effect of immunization with tick cement protein 64TRP (Trimnell et al, 2002(Trimnell et al, , 2003(Trimnell et al, , 2005Havlíková et al, 2009) in comparison with the commercial TBEV vaccine and antitick vaccine TickGard was based on the mouse laboratory model in which mice were infested with TBEV infected ticks (Labuda et al, 2006). Neither commercial vaccine was as effective as 64TRP in controlling TBEV transmission from the infected tick to uninfected co-feeding ticks.…”

Section: Non-viraemic Transmissionmentioning

confidence: 99%

Non-viraemic transmission of tick-borne viruses

Havlíková¹,

Ličková²,

Klempa³

2013

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Summary. -Tick-borne viruses are causative agents of several important human diseases. Tick-borne encephalitis virus (TBEV) is the most prominent representative considered medically to be the most important arbovirus (arthropod-borne virus) in Europe and northern Asia. Tick-borne virus transmission cycles are determined by the interactions between viruses, vectors, and their vertebrate hosts. Several mechanisms of tick-borne virus circulation in nature are currently considered to include transovarial transmission via the eggs from an infected female tick to its offspring, "viraemic" transmission between host and tick via feeding on a viraemic, infectious vertebrate hosts, and the virus transmission between co-feeding ticks, termed non-viraemic transmission (NVT). For NVT, the local skin site where ticks aggregately feed is an important focus of viral replication where migratory immune cells provide a vehicle for virus transmission from infected to uninfected co-feeding ticks. For TBEV at least, NVT is an important mechanism of virus maintenance in nature and offers explanations for some specific aspects of tick-borne virus ecology such as focal virus distribution and vector competency of particular tick species.Keywords: routes of transmission; non-viraemic transmission; arbovirus; tick-borne viruses; co-feeding; tick-borne encephalitis virus E-mail: virusaha@savba.sk; phone: +421-2-59302428. Abbreviations: ASFV = African swine fever virus; CCH-FV = Crimean-Congo hemorrhagic fever virus; NVT = nonviraemic transmission; SAT = saliva-activated transmission; SGE = salivary gland extract; THOV = Thogoto virus; TBEV = tickborne encephalitis virus

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Functional role of 64P, the candidate transmission-blocking vaccine antigen from the tick, Rhipicephalus appendiculatus

Cited by 37 publications

References 38 publications

Tick vaccines: current status and future directions

Tick vaccines: current status and future directions

Tick attachment cement – reviewing the mysteries of a biological skin plug system

Non-viraemic transmission of tick-borne viruses

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