Functional role of CD26 on human B lymphocytes

Bühling, Frank; Junker, Uwe; Reinhold, Dirk; Neubert, Klaus; Jäger, Lothar; Ansorge, Siegfried

doi:10.1016/0165-2478(94)00230-o

Cited by 105 publications

(90 citation statements)

References 11 publications

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“…Thus, our data suggest that malignant B-cells, like activated normal B-cells, express at their surface a CD26/DPP IV molecule similar to the major form of CD26/DPP IV present on activated T-cells. In vitro treatment of activated T-and B-cells with specific DPP IV inhibitors led to cell growth suppression and a decrease in cytokine or IgM production (Schšn et al, 1987;Flentke et al, 1991;BŸhling et al, 1994). A specific inhibitor of DPP IV prodipine has recently been shown to abrogate in vivo systemic IgM allo-Ab responses in rats (De Meester et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

“…As illustrated in Figure 2, isolated CD20 + CD23 Ð B-cells from seven healthy donors were found CD26 Ð . BŸhling et al (1994) previously found that SAC increased CD26 expression on B-cells. Moreover, the physiological B-cell stimulatory factor IL-4 is known to increase the expression of a variety of molecules on B-cells including CD23 (Kolb et al, 1991).…”

Section: Differential Expression Of Cd26 Molecules On the Surface Of mentioning

confidence: 99%

“…By using one-colour FACS analysis, it was previously suggested that a small subset (² 5%) of normal peripheral B-cells reacted with the mAb Ta1 raised against the CD26 molecule from activated T-cells (BŸhling et al, 1994). In this study, two-colour FACS analysis was used to determine the levels of Ta1/CD26 expression on CD20 + Bcells.…”

Section: Differential Expression Of Cd26 Molecules On the Surface Of mentioning

confidence: 99%

“…CD26/DPP IV is abundantly expressed in kidney, lung, small intestine and liver (Fleisher, 1994). In the immune system, CD26/DPP IV is found primarily at low density on subsets of CD4 + and CD8 + T-cells and on CD3 + medullary thymocytes (Fleisher, 1994) and on activated natural killer (NK) and B-cells (BŸhling et al, 1994;Fleisher 1994).…”

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Constitutive expression of CD26/dipeptidylpeptidase IV on peripheral blood B lymphocytes of patients with B chronic lymphocytic leukaemia

et al. 1999

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SummaryWe have investigated the expression of the ectoenzyme dipeptidylpeptidase IV (DPP IV)/CD26 on lymphocytes obtained from patients with B chronic lymphocytic leukaemia (B-CLL) and compared it with healthy subjects. Using two-colour immunofluorescence analysis with CD26 and CD20 or CD23 monoclonal antibodies, CD26 was found undetectable on peripheral resting B-cells (CD20 + CD23 -) from normal donors whereas it was expressed on B-cells activated in vitro with interleukin (IL)-4 and Staphylococcus aureus strain cowan I (CD20 + CD23 + ). The expression of CD26 on leukaemic B-cells (CD20 + CD23 + ) was clearly induced in 22 out of 25 patients examined. Consequently, induced levels of CD26 cell surface expression on either normal activated and malignant B-cells coincided with the enhancement of DPP IV activity detected on the surface of these cells. Reverse transcription polymerase chain reaction analyses showed that the transcript levels of the CD26 gene was higher in normal activated B-cells and B-CLL cells than in resting B-cells, suggesting that CD26 was expressed at the level of transcriptional activation. These observations provide evidence of the abnormal expression of DPPIV/CD26 in B-CLL which, therefore, may be considered as a novel marker for B-CLL. Further investigation in relation to CD26 expression and other B malignancies needs to be defined.

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Section: Resultsmentioning

confidence: 99%

Section: Differential Expression Of Cd26 Molecules On the Surface Of mentioning

confidence: 99%

Section: Differential Expression Of Cd26 Molecules On the Surface Of mentioning

confidence: 99%

mentioning

confidence: 99%

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Constitutive expression of CD26/dipeptidylpeptidase IV on peripheral blood B lymphocytes of patients with B chronic lymphocytic leukaemia

et al. 1999

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“…DPPIV/CD26 is expressed on the cell surface of CD4 ϩ T lymphocytes as well as in B cells and natural killer cells (Buhling et al, 1994(Buhling et al, , 1995Morimoto et al, 1989). DPPIV activates T lymphocytes through stimulation of the T-cell receptor and enhances chemotactic migration of T cells (Iwata et al, 1999;Morimoto and Schlossman, 1998).…”

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confidence: 99%

Involvement of Dipeptidyl Peptidase IV in Immune Complex–Mediated Glomerulonephritis

Shinosaki

Kobayashi

Kimura

et al. 2002

Laboratory Investigation

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SUMMARY:Dipeptidyl peptidase IV (DPPIV) is widely expressed in many tissues; however, its precise biological function is poorly understood. One of its possible physiologic roles is an involvement in the immune system, which plays a pivotal role in the pathogenesis of glomerulonephritis. The present study focused on the involvement of DPPIV in immune complex-mediated glomerulonephritis. Experimental nephritis was induced by anti-Thy-1.1 monoclonal antibody E30 using Wistar or F344 rats. The application of a new monoclonal antibody against DPPIV, F16, completely suppressed E30-induced proteinuria and mesangial proliferation in Wistar rats, whereas these preventive effects of F16 were not observed in F344 rats, which spontaneously lack DPPIV protein. Treatment with F16 inhibited glomerular deposition of complement C3 and complement C4 after the binding of E30 to the mesangial cell surface. Because the preventive effect of F16 was attributable to suppression of the complement cascade, we examined its influences on complement-dependent mesangial cell lysis in vitro. We discovered that the complement cascade was markedly inactivated in F16-treated Wistar rat serum but not in F16-treated F344 rats. These results indicate that DPPIV may play a somewhat crucial role in regulating the complement cascade and that inhibition of DPPIV may serve as a new target for preventing complement-dependent tissue injury. (Lab Invest 2002, 82:505-513).

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CD26 expression and dipeptidyl peptidase IV activity in an aggressive hepatosplenic T-cell lymphoma

et al. 1998

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Functional role of CD26 on human B lymphocytes

Cited by 105 publications

References 11 publications

Constitutive expression of CD26/dipeptidylpeptidase IV on peripheral blood B lymphocytes of patients with B chronic lymphocytic leukaemia

Constitutive expression of CD26/dipeptidylpeptidase IV on peripheral blood B lymphocytes of patients with B chronic lymphocytic leukaemia

Involvement of Dipeptidyl Peptidase IV in Immune Complex–Mediated Glomerulonephritis

CD26 expression and dipeptidyl peptidase IV activity in an aggressive hepatosplenic T-cell lymphoma

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