Functional role of vitronectin in breast cancer

Bera, Alakesh; Subramanian, Marappa G.; Karaian, John; Eklund, Michael; Radhakrishnan, Surya; Gana, Nahbuma; Rothwell, Stephen W.; Pollard, Harvey B.; Hu, Hai; Shriver, Craig D.; Srivastava, Meera

doi:10.1371/journal.pone.0242141

“…VTN induces cell differentiation in endoderm cells. In human plasma, malignant differentiation of lung and breast cancer has been reported [41,42] VTN mediates its effects predominantly through αVβ3 integrin and as a result, causes a change in the β-catenin localization. 43 Primary lung cancer has been associated with overexpression of β-catenin protein in tissues.…”

Section: Resultsmentioning

confidence: 99%

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Romero¹,

Cavalcante²,

Monteiro-Moreira³

et al. 2022

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0

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Myelodysplastic syndromes (MDS) are diseases that occur when blood-producing cells in the bone marrow are damaged; such damage can affect one or more types of blood cells. Common types of MDS are refractory anemia with ring sideroblasts and refractory anemia with excess blasts (MDS-RS and MDS-EB, respectively). This work analyzed the proteomics of the medullary plasma of 10 patients with MDS-RS and MDS-EB compared to healthy control people. Overexpressed proteins that may be potential candidates for biological markers for the evaluation, study, and diagnosis of these diseases have been identified. These samples were subjected to immunodepleting, concentrated, and digested for further analysis by mass spectrometry. The ratios between selected groups and healthy people were calculated. Seven overexpressed proteins in both syndromes were identified as potential biomarker candidates: vitronectin (VTN), (2) fibrinogen (FGA), (3) pregnancy zone protein (PZP), (4) kininogen (KNG1), (5) immunoglobulin lambda chain (IGLL1), (6) complement factor C4b, and (7) hemopexin (HPX). A modified affinity chromatographic column with lectin frutalin (FTL) was used for non-depleted samples. Immunoglobulin M (IgM) was expressed in the samples from both syndromes. Surprisingly, IgM from patients with syndromes was over retained on the frutalin (FTL) column when compared with the control group. We further hypothesized that over retention of this protein by the FTL is due to the presence of α-galactosidic residues in the IgM of MDS-RS and MDS-EB patients. Differential recognition of proteins on non-depleted samples from the use of FTL appears to be a powerful tool for proteomic analysis.

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“…VTN induces cell differentiation in endoderm cells. In human plasma, malignant differentiation of lung and breast cancer has been reported [41,42] VTN mediates its effects predominantly through αVβ3 integrin and as a result, causes a change in the βcatenin localization. 43 Primary lung cancer has been associated with overexpression of βcatenin protein in tissues.…”

Section: Resultsmentioning

confidence: 99%

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Romero¹,

Romo.²,

Cavalcante³

et al. 2022

Preprint

0

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Myelodysplastic syndromes (MDS) are diseases that occur when blood-producing cells in the bone marrow are damaged; such damage can affect one or more types of blood cells. Common types of MDS are refractory anemia with ring sideroblasts and refractory anemia with excess blasts (MDS-RS and MDS-EB, respectively). This work analyzed the proteomics of the medullary plasma of 10 patients with MDS-RS and MDS-EB compared to healthy control people. Overexpressed proteins that may be potential candidates for biological markers for the evaluation, study, and diagnosis of these diseases have been identified. These samples were subjected to immunodepleting, concentrated, and digested for further analysis by mass spectrometry. The ratios between selected groups and healthy people were calculated. Seven overexpressed proteins in both syndromes were identified as potential biomarker candidates: vitronectin (VTN), (2) fibrinogen (FGA), (3) pregnancy zone protein (PZP), (4) kininogen (KNG1), (5) immunoglobulin lambda chain (IGLL1), (6) complement factor C4b, and (7) hemopexin (HPX). A modified affinity chromatographic column with lectin frutalin (FTL) was used for non-depleted samples. Immunoglobulin M (IgM) was expressed in the samples from both syndromes. Surprisingly, IgM from patients with syndromes was over retained on the frutalin (FTL) column when compared with the control group. We further hypothesized that over retention of this protein by the FTL is due to the presence of α-galactosidic residues in the IgM of MDS-RS and MDS-EB patients. Differential recognition of proteins on non-depleted samples from the use of FTL appears to be a powerful tool for proteomic analysis.

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“…VTN is a part of the extracellular matrix that promotes integrin signaling. VTN, which is downstream of VEGF/VEGFR and PI3K/AKT signaling, promotes cell migration and metastasis in breast cancer [33,34,35]. SBSN is an oncoprotein that promotes tumorigenicity by increasing Wnt/β-catenin signaling [36,37].…”

Section: Discussionmentioning

confidence: 99%

Role of Differentially Expressed Proteins in Acquired Resistance to Cdk4/6 Inhibitor in Breast Cancer

Kumar

¹

,

Prasad

²

,

Singh

³

et al. 2021

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0

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CDK4/6 inhibitors (Abemaciclib, Ab and Palbociclib, Pb) stop the G1-phase in cell-cycle being used to cure advanced stage of breast cancer (BC). Acquired resistance is a major challenge in BC therapy. The molecular signature of the therapy resistance for Ab and Pb drugs in BC should be explored. Here, we developed Ab/Pb-resistant cell-models and explored the molecular changes. Drug’s resistance cells were developed in MCF-7 cells by continuous drug treatment and it was confirmed by MTT-assay, PI-staining-microscopy, and real-time-qPCR. Global proteome profiling done by Labelled-free-Proteome-Orbitrap-Fusion-MS-MS technique. Bioinformatics tools used to analyse the proteome data. Ab-resistant and Pb-resistant MCF-7 cells showed increased tolerance for the respective drug. The BCL-2 and MCL-1 survival genes were up-regulated, while the apoptosis genes BAD, BAX, CASP-3 and PARP-1were down-regulated in the resistant cells. Expression of the MDR-1, ABCG2, ESR-1, CDK4, CDK6, and Cyclin-D1 genes were increased in both resistance cells. For proteomics, 237 and 239 proteins were expressed differently in the resistant Ab and Pb cells, respectively. The NUDT5, PEPD, ABAT, ATP1B1, GGCT, and SELENBP1 proteins were down-regulated and the SBSN, HSD17B10, CD9, PDIA3, PSMB4, SLC2A1, and VTN proteins were up-regulated in Ab-resistant cells. The NUDT5, PEPD, and GGCT proteins were down-regulated, while CD47, HIST1H2BN, LMNA, VTN, PSMB5, HBB, PSMA7, FLNB, PRDX4, VDAC1, GOT2, HSPA5, SERPINH1, EIF4A2, FTH, and VIM proteins were up-regulated in Pb-resistant cells. These proteins are a new set of prognostic markers and drug targets for overcoming the respective drug resistance. However, it is necessary to perform an in vivo or clinical assessment.

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Functional role of vitronectin in breast cancer

Cited by 33 publications

References 30 publications

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Role of Differentially Expressed Proteins in Acquired Resistance to Cdk4/6 Inhibitor in Breast Cancer

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