Functional Separation of the Na-K Exchange Pump from the Volume Controlling Mechanism in Enlarged Duck Red Cells

Kregenow, Floyd M.

doi:10.1085/jgp.64.4.393

Cited by 34 publications

(13 citation statements)

References 20 publications

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“…Cell swelling has been reported to activate (K + Cl) cotransport in avian erythrocytes (Kregenow, 1974;Kregenow and Caryk, 1979;Haas and McManus, 1985). The swelling-activated, Cl-dependent K transport in sheep erythrocytes Dunham and Ellory, 1981) is also postulated to be (K + Cl) cotransport (Lauf, 1985).…”

Section: Discussionmentioning

confidence: 98%

“…In particular, swelling has been reported to activate Cldependent K transport in avian (Kregenow, 1974 ;Kregenow and Caryk, 1979 ;Haas and McManus, 1985), sheep Dunham and Ellory, 1981 ;Lauf, 1984), dog (Parker, 1983), and toadfish erythrocytes (Lauf, 1985), K/H exchange in Amphiuma erythrocytes (Gala, 1983), K conductance in human lymphocytes (Sarkadi et al ., 1985) and Ehrlich ascites tumor cells (Hoffmann et al ., 1984), and K loss through an unidentified mechanism in flounder erythrocytes (Gala, 1977).…”

Section: Introductionmentioning

confidence: 99%

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Volume-sensitive K transport in human erythrocytes.

Kaji¹

1986

The Journal of General Physiology

110

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Studies have been carried out on human erythrocytes to examine the alterations of K transport induced by swelling or shrinking the cells by osmotic and isosmotic methods. Hypotonic swelling of erythrocytes (relative cell volume, 1 .20) resulted in a striking, four-to fivefold augmentation in the ouabain-resistant K influx over the value obtained at a normal cell volume . Shrinking the cells in hypertonic media resulted in a small but statistically significant reduction in K influx . Three different methods of varying cell volume gave similar results. These include the addition of sucrose and of NaCI to hypotonic media and the isosmotic (nystatin) method . The major fraction of the K influx in swollen cells is specific in its requirement for CI or Br and is not supported by thiocyanate, iodide, nitrate, methylsulfate, or acetate. Bumetanide (0

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Volume-sensitive K transport in human erythrocytes.

Kaji¹

1986

The Journal of General Physiology

110

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“…Thus many invertebrate tissues lose cellular amino acids when exposed to hyposmotic media (Schoffeniels, 1967). Recently it has been shown that duck erythrocytes exposed tohyposmotic media lose potassium and thereby chloride (Kregenow, 1971(Kregenow, , 1973(Kregenow, , 1974 and that these losses are not affected by ouabain (Kregenow, 1971). Dellasega & Grantham (1973) have suggested that the regulation of cellular volume in renal tubular cells exposed to hyposmotic medium may involve a similar loss of potassium and chloride.…”

Section: Introductionmentioning

confidence: 99%

“…More recently in a preliminary report they have suggested that losses of both potassium and sodium may be involved (Grantham, Lowe & Dellasega, 1974). Thus it has been suggested that cellular potassium plays a central role in protecting cells from undue swellinginhyposmotic medium, perhaps as a result ofchanges inmembrane permeability to potassium under these conditions (Kregenow, 1974).…”

Section: Introductionmentioning

confidence: 99%

The regulation of cellular volume in renal cortical slices incubated in hyposmotic medium.

Hughes¹,

Macknight²

1976

The Journal of Physiology

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SUMMARY1. Changes in water and ion contents of renal cortical slices from rat, rabbit and guinea-pig incubated in medium of a half normal osmolarity were followed over 60 min incubation at 25°C.2. Metabolizing slices gained water and lost sodium and chloride but not potassium. These changes reflected in part changes in cellular composition, and were complete within 5 min. A new steady-state was then maintained for a further 55 min.3. Slices initially incubated for 60 min with ouabain, also lost sodium and chloride when transferred to the hyposmotic medium but did not, in the first minutes, lose any further potassium. Ouabain, in concentrations sufficient to produce maximal inhibition of the Na-K ATPase, did not cause additional cellular swelling in the hyposmotic medium. 4. The results offer no support for the claim that cellular volume in metabolizing renal cortical cells exposed to hyposmotic media is regulated by loss of cellular potassium. They suggest that under such conditions the regulation of volume involves the same ouabsin-insensitive mechanism as is seen in cells incubated in isosmotic medium.

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“…The study of volume regulation by duck red blood cells was pioneered by Kregenow [164,167], who showed that following osmotic swelling the duck red cell regulates its volume back to control levels (RVD) in about 90 min. This RVD results from activation of a transport system which mediates an efflux of K + and CI-from the cells driven by the K + concentration gradient [164].…”

Section: Duck Red Cellsmentioning

confidence: 99%