Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells

Mergler, Stefan; Mertens, C; Valtink, Monika; Reinach, Peter S.; Székely, Violeta Castelo; Slavi, Nefeli; Garreis, Fabian; Abdelmessih, Suzette; Türker, Ersal; Fels, Gabriele; Pleyer, Uwe

doi:10.1016/j.exer.2013.10.003

Cited by 29 publications

(34 citation statements)

References 42 publications

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“…However, 10 μM La 3+ was ineffective in blocking menthol-activated TRPM8 in an overexpression system [ 210 ]. Consistently, TRPM8 channels in corneal epithelial cells were recently found to be partially inhibited by 100 μM Gd 3+ [ 133 ]. Compared to the weak sensitivity to lanthanides of mammalian TRPM channels, the TRPM channels of C. elegans , GTL-1 and GON-2, are rather sensitive to La 3+ as they are inhibited with an IC 50 of approximately 5 μM [ 232 ].…”

Section: Inhibition Of Trp Channels By Metal Ionsmentioning

confidence: 58%

Permeation, regulation and control of expression of TRP channels by trace metal ions

Bouron

Kiselyov

Oberwinkler

2014

Pflugers Arch - Eur J Physiol

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Transient receptor potential (TRP) channels form a diverse family of cation channels comprising 28 members in mammals. Although some TRP proteins can only be found on intracellular membranes, most of the TRP protein isoforms reach the plasma membrane where they form ion channels and control a wide number of biological processes. There, their involvement in the transport of cations such as calcium and sodium has been well documented. However, a growing number of studies have started to expand our understanding of these proteins by showing that they also transport other biologically relevant metal ions like zinc, magnesium, manganese and cobalt. In addition to this newly recognized property, the activity and expression of TRP channels can be regulated by metal ions like magnesium, gadolinium, lanthanum or cisplatin. The aim of this review is to highlight the complex relationship between metal ions and TRP channels.

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Section: Inhibition Of Trp Channels By Metal Ionsmentioning

confidence: 58%

Permeation, regulation and control of expression of TRP channels by trace metal ions

Bouron

Kiselyov

Oberwinkler

2014

Pflugers Arch - Eur J Physiol

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“…TRPM8 functional expression has been recently suggested in ECs (Johnson et al, 2009; Mergler et al, 2013; Sun et al, 2014). Our data support TRPM8 expression in ECs by showing not only TRPM8 protein expression in different ECs but also its dramatic down-regulation in different clones of tumor-derived ECs.…”

Section: Discussionmentioning

confidence: 99%

TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1

Genova

Grolez

Camillo

et al. 2017

Journal of Cell Biology

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“…Similarly, Reed et al saw best detachment of bovine aortic endothelial cells after incubation at 4 °C, while Okano et al observed efficient cell detachment above 20 °C, from pure PNiPAAm coatings [48, 49]. Since HCEC have thermo-sensitive ion channels responding to cold or heat with an increased Ca 2+ influx [50–52], cold-induced detachment may influence cell survival due to Ca 2+ -activated signaling. It is known that hypothermic storage of donor corneas at 4 °C, which is usually performed for up to 14 days, can lead to elevated cell death of corneal endothelial cells [53–55], e.g.…”

Section: Discussionmentioning

confidence: 99%

Thermo-responsive cell culture carriers based on poly(vinyl methyl ether)—the effect of biomolecular ligands to balance cell adhesion and stimulated detachment

Teichmann

Nitschke

Pette

et al. 2015

Science and Technology of Advanced Materials

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Two established material systems for thermally stimulated detachment of adherent cells were combined in a cross-linked polymer blend to merge favorable properties. Through this approach poly(N-isopropylacrylamide) (PNiPAAm) with its superior switching characteristic was paired with a poly(vinyl methyl ether)-based composition that allows adjusting physico-chemical and biomolecular properties in a wide range. Beyond pure PNiPAAm, the proposed thermo-responsive coating provides thickness, stiffness and swelling behavior, as well as an apposite density of reactive sites for biomolecular functionalization, as effective tuning parameters to meet specific requirements of a particular cell type regarding initial adhesion and ease of detachment. To illustrate the strength of this approach, the novel cell culture carrier was applied to generate transplantable sheets of human corneal endothelial cells (HCEC). Sheets were grown, detached, and transferred onto planar targets. Cell morphology, viability and functionality were analyzed by immunocytochemistry and determination of transepithelial electrical resistance (TEER) before and after sheet detachment and transfer. HCEC layers showed regular morphology with appropriate TEER. Cells were positive for function-associated marker proteins ZO-1, Na+/K+-ATPase, and paxillin, and extracellular matrix proteins fibronectin, laminin and collagen type IV before and after transfer. Sheet detachment and transfer did not impair cell viability. Subsequently, a potential application in ophthalmology was demonstrated by transplantation onto de-endothelialized porcine corneas in vitro. The novel thermo-responsive cell culture carrier facilitates the generation and transfer of functional HCEC sheets. This paves the way to generate tissue engineered human corneal endothelium as an alternative transplant source for endothelial keratoplasty.

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Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells

Cited by 29 publications

References 42 publications

Permeation, regulation and control of expression of TRP channels by trace metal ions

Permeation, regulation and control of expression of TRP channels by trace metal ions

TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1

Thermo-responsive cell culture carriers based on poly(vinyl methyl ether)—the effect of biomolecular ligands to balance cell adhesion and stimulated detachment

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