Functional Silver Nanocomposites as Broad-Spectrum Antimicrobial and Biofilm-Disrupting Agents

Guo, Qianqian; Zhao, Yu; Dai, Xiaomei; Zhang, Tianqi; Yu, Yunjian; Zhang, Xinge; Li, Chaoxing

doi:10.1021/acsami.7b02775

Cited by 65 publications

(52 citation statements)

References 70 publications

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“…Although the antibacterial nanoparticles are considered as promising candidates for addressing AMR, multiple cycles of treatment below lethal concentration also led to gradual increases in MIC of AgNPs, which exacerbates our concerns about whether biofilm exhibits tolerance and resistance to it. In this study, the results showed that AgNPs possessed the strong dose‐dependent anti‐biofilm capacities, which conformed to previous work . It is worth noting that P aeruginosa biofilm became more resistant to AgNPs.…”

Section: Discussionsupporting

confidence: 91%

Silver nanoparticles exert concentration‐dependent influences on biofilm development and architecture

et al. 2019

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Objectives:To investigate the impact of silver nanoparticles (AgNPs) on the biofilm growth and architecture.Materials and methods: Silver nitrate was reduced by d-maltose to prepare AgNPs in the presence of ammonia and sodium hydroxide. The physicochemical properties of AgNPs were characterized by transmission electron microscopy, ultraviolet-visible spectroscopy and inductively coupled plasma mass spectrometry. The development of biofilm with and without AgNPs was explored by crystal violet stain. The structures of mature biofilm were visually studied by confocal laser scanning microscopy and scanning electron microscopy. Bacterial cell, polysaccharide and protein within biofilm were assessed quantitatively by colony-counting method, phenol-sulphuric acid method and Bradford assay, respectively. Results:The spherical AgNPs (about 30 nm) were successfully synthesized. The effect of AgNPs on Pseudomonas aeruginosa biofilm development was concentrationdependent. Biofilm was more resistant to AgNPs than planktonic cells. Low doses of AgNPs exposure remarkably delayed the growth cycle of biofilm, whereas high concentration (18 μg/mL) of AgNPs fully prevented biofilm development. The analysis of biofilm architecture at the mature stage demonstrated that AgNPs exposure at all concentration led to significant decrease of cell viability within treated biofilms.However, sublethal doses of AgNPs increased the production of both polysaccharide and protein compared to control, which significantly changed the biofilm structure.Conclusions: AgNPs exert concentration-dependent influences on biofilm development and structure, which provides new insight into the role of concentration played in the interaction between antibacterial nanoparticles and biofilm, especially, an ignored sublethal concentration associated with potential unintended consequences.

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Section: Discussionsupporting

confidence: 91%

Silver nanoparticles exert concentration‐dependent influences on biofilm development and architecture

et al. 2019

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“…Moreover, increase of fungal inhibition depends on NPs concentration as well as particle size, as the smaller particle size prompts extensive membrane permeability and cell destruction . Smaller particle size has large surface area that could be firmly absorbed on the surface of the microbial cells so as to dismantle the membrane, which leads to effluence of intracellular components, consequently killing the microorganisms …”

Section: Resultsmentioning

confidence: 99%

Organometallic assembling of chitosan‐Iron oxide nanoparticles with their antifungal evaluation againstRhizopus oryzae

Saqib

Zaman

Ullah

et al. 2019

Applied Organom Chemis

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The ever-increasing resistance of plant microbes towards fungicides and bactericides has been causing serious threat to plant production in recent years. For the development of an effective antifungal agent, we introduce a novel hydrothermal protocol for synthesis of chitosan iron oxide nanoparticles (CH-Fe 2 O 3 NPs) using acetate buffer of low pH 5.0 for intermolecular interaction of Fe 2 O 3 NPs and CH. The composite structure and elemental elucidation were carried out by using X-ray power diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), Transmission Electron Microscopy (TEM), Fourier Transformed Infrared Spectroscopy (FTIR) and Ultraviolet Visible Absorption Spectroscopy (UV-vis spectroscopy). Additionally, antifungal activity was evaluated both In vitro and In vivo against Rhizopus oryzae which is causing fruit rot disease of strawberry. We compared different concentrations (0.25%, 0.50%, 075% and 1%) of CH-Fe 2 O 3 NPs and 50% synthetic fungicide (Matalyxal Mancozab) to figure out suitable concentration for application in the field. XRD analysis showed a high crystalline nature of the NPs with average size of 52 nanometer (nm). SEM images revealed spherical shape with size range of 50-70 nm, whereas, TEM also revealed spherical shape, size ranging from 0 nm to 80 nm. EDX and FTIR results revealed presence of CH on surface of Fe 2 O 3 NPs. The band gap measurement showed peak 317-318 nm for bare Fe 2 O 3 NPs and CH-Fe 2 O 3 NPs respectively. Antifungal activity in both In vitro and In vivo significantly increased with increase in concentration. The overall results revealed high synergetic antifungal potential of organometallic CH-Fe 2 O 3 NPs against Rhizopus oryzae and suggest the use of CH-Fe 2 O 3 NPs against other Phyto-pathological diseases due to biodegradable nature.

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“…[12] Taken the biocompatibility into consideration, the phase transfer agent should be removed from GOHCM before biological applications, because of the cytotoxicity of cationic surfactants via destroying the electric balance between the inner and outer of the cell membrane. [32] After tested with a series of organic solvents, ethanol was found to be an efficient and safe extractant. As shown in Figure S8 in the Supporting Information, the hierarchical structures consisting of microscale honeycomb patterns and nanoscale textures were well maintained because of the strong affinity between adjacent graphene oxide sheets.…”

Section: Preparation Of Self-assembled Go Honeycomb Microarraymentioning

confidence: 99%

Self‐Assembled GO Honeycomb Microarray for Selective Cancer Cell Capture and Single Cell Analysis of Proteolytic Expression

Chen

2020

Adv Healthcare Materials

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Proteolytic enzymes expressed by circulating tumor cells are proved to facilitate their invasion into multiple organs via cleaving natural ECM networks, leading to consequent metastasis colonization and malignant lethality. Recent evidence suggests the rare metastasis initiating cells with higher proteolytic levels among circulating tumor cells (CTCs) may strongly increase the risk of metastasis. Beyond selective CTC capture, the heterogeneity in proteases expression provides a promising indicator for metastasis happening. To this end, the graphene oxide (GO) honeycomb microarray with single CTC matched sizes is fabricated via the self-assembly breath figure approach, which serves as an integrated protocol for selective CTC capture and single-cell analysis of protease activity. Contributing to synergistic effects of structure and chemistry, CTCs can be efficiently isolated and individually trapped in each honeycomb hole. Meanwhile, the crosstalk among CTCs can be erased by blocking direct cell-to-cell contact, which offers promising potentials in the single-cell analysis of protease expression. Integrating specific capture and in situ analysis of single CTCs on GO micropatterned surface is of significant importance in various biological and clinical applications such as cancer diagnostics and cancer therapeutic evaluation.

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Functional Silver Nanocomposites as Broad-Spectrum Antimicrobial and Biofilm-Disrupting Agents

Cited by 65 publications

References 70 publications

Silver nanoparticles exert concentration‐dependent influences on biofilm development and architecture

Silver nanoparticles exert concentration‐dependent influences on biofilm development and architecture

Organometallic assembling of chitosan‐Iron oxide nanoparticles with their antifungal evaluation againstRhizopus oryzae

Self‐Assembled GO Honeycomb Microarray for Selective Cancer Cell Capture and Single Cell Analysis of Proteolytic Expression

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