Functional stimulation of graft nerves has minor effects on insulin release from transplanted rat pancreatic islets

Jansson, Leif; Kampf, Caroline; Källskog, Örjan

doi:10.3109/03009734.2013.818601

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…Therefore, the fractional pericyte numbers were lower in the SC-islets compared with the human islet grafts. For islets implanted beneath the renal capsule, there is also less nerve functionality compared with other investigated sites ( 50 ). Finally, testing of graft functionality was limited to glucose-stimulated human C-peptide and did not include experiments with reversal of diabetes.…”

Section: Discussionmentioning

confidence: 99%

Efficient Vascular and Neural Engraftment of Stem Cell–Derived Islets

Thorngren,

Brboric,

Vasylovska

et al. 2024

Diabetes

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Pluripotent stem cell-derived islets (SC-islets) now emerge as a new source for beta-cell replacement therapy. While the function of human islet transplants is hampered by excessive cell death post-transplantation, contributing factors include inflammatory reactions, insufficient revascularization and islet amyloid formation, there is a gap in knowledge on the engraftment process of the SC-islets. In this experimental study, we investigated the engraftment capability of SC-islets at three months post-transplantation, and observed that the cell apoptosis rates were lower, but the vascular density was similar in SC-islets to that of human islets. While the human islet transplant vascular structures were a mixture of remnant donor endothelium and ingrowing blood vessels, the SC-islets contained ingrowing blood vessels only. The oxygenation of the SC-islet grafts was twice as high as in the corresponding grafts of human islets, suggesting better vascular functionality. Similar to the blood vessel ingrowth, also the reinnervation of the SC-islets was four- to five-fold higher than the human islets. Both SC-islets and the human islets contained amyloid at one and three months post-transplantation. We conclude that the vascular and neural engraftment of SC-islets is superior to human islets, but that grafts of both origins develop amyloid with potential long-term consequences.

show abstract

Section: Discussionmentioning

confidence: 99%

Efficient Vascular and Neural Engraftment of Stem Cell–Derived Islets

Thorngren,

Brboric,

Vasylovska

et al. 2024

Diabetes

View full text Add to dashboard Cite

show abstract

“…Therefore, the fractional pericyte numbers were lower in the SC-islets compared to human islet grafts. For islets implanted beneath the renal capsule, there are also less nerve functionality than at other investigated sites (50). Finally, testing of graft functionality was limited to glucose-stimulated human C-peptide and did not include experiments with reversal of diabetes.…”

Section: Discussionmentioning

confidence: 99%

<b>Efficient vascular and neural engraftment of stem cell-derived islets</b>

Thorngren,

Brboric,

Vasylovska

et al. 2024

Preprint

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<p dir="ltr">Pluripotent stem cell-derived islets (SC-islets) now emerge as a new source for beta-cell replacement therapy. While the function of human islet transplants is hampered by excessive cell death post-transplantation, contributing factors include inflammatory reactions, insufficient revascularization and islet amyloid formation, there is a gap in knowledge on the engraftment process of the SC-islets. In this experimental study, we investigated the engraftment capability of SC-islets at three months post-transplantation, and observed that the cell apoptosis rates were lower, but the vascular density was similar in SC-islets to that of human islets. While the human islet transplant vascular structures were a mixture of remnant donor endothelium and ingrowing blood vessels, the SC-islets contained ingrowing blood vessels only. The oxygenation of the SC-islet grafts was twice as high as in the corresponding grafts of human islets, suggesting better vascular functionality. Similar to the blood vessel ingrowth, also the reinnervation of the SC-islets was four- to five-fold higher than the human islets. Both SC-islets and the human islets contained amyloid at one and three months post-transplantation. We conclude that the vascular and neural engraftment of SC-islets is superior to human islets, but that grafts of both origins develop amyloid with potential long-term consequences.</p>

show abstract