Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases

Sylvestre-Gonon, Elodie; Law, Simon R.; Schwartz, Mathieu; Robe, Kevin; Keech, Olivier; Didierjean, Claude; Dubos, Christian; Rouhier, Nicolas; Hecker, Arnaud

doi:10.3389/fpls.2019.00608

Cited by 80 publications

(78 citation statements)

References 176 publications

(224 reference statements)

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“…It is a very large family of about 10 classes, these differing both structurally and functionally. This family is implicated in the vacuolar sequestration of flavonoids, as well as in xenobiotic detoxification (reviewed in [ 95 ]). Less recently, it has been noted how GST tissue-specific expression can be due to exposure to chemical treatments and to biotic or abiotic stresses [ 6 ].…”

Section: Resultsmentioning

confidence: 99%

Target-Genes Reveal Species and Genotypic Specificity of Anthocyanin Pigmentation in Citrus and Related Genera

Catalano

Ciacciulli

Salonia

et al. 2020

Genes

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Background: Anthocyanin pigmentation characterizes a number of tissues of Citrus and its relatives. The gain and loss of pigmentation is intriguing and is inherited variously among species. Methods: Citrus germplasm was used to investigate the anthocyanin pigmentation of tissues never before considered, including stamen, style and stigma, and of young leaves, petals, rind and flesh of 28 genotypes belonging to 14 species. Citrus genotypes encompassed citron, lemon, sweet orange, lime, and Citrus relatives included Microcitrus, Murraya, and Severinia. A relative qRT-PCR analysis was carried out on the structural and regulatory genes: phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3′-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), uridine diphosphate glucose flavonoid glucosyl-transferase (UFGT), glutathione S-transferase (GST), Ruby and Noemi. Image analysis and a genomic approach were employed to evaluate how the red pigmentation is inherited among tissues and species. Results: Pigmentation of young leaves and petals is specific to citron and its hybrids. Ruby controls the pigmentation of petals, but not of leaves. The red color of the rind and flesh is a trait that particularly characterizes a diversity of sweet oranges, citron hybrids and Citrus relatives. Color expression depends on external factors and also on developmental stage. The coloration of stamen and style is citron-specific, while a red stigma is exclusive to Moro orange and its hybrids. Conclusion: It is hypothesized that there is a relationship among Citrus species and genes controlling anthocyanin pigmentation.

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Section: Resultsmentioning

confidence: 99%

Target-Genes Reveal Species and Genotypic Specificity of Anthocyanin Pigmentation in Citrus and Related Genera

Catalano

Ciacciulli

Salonia

et al. 2020

Genes

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“…It is well established that GSTU7 is strongly induced under different abiotic stress situations and that many GSTs are induced by SA and other phytohormones (Marrs, 1996; Sylvestre-Gonon et al, 2019). Our results confirm that SA induces the expression of GSTU7 within a few hours of application.…”

Section: Discussionmentioning

confidence: 99%

“…It is established that GSTUs and GSTFs are subject to complex transcriptional and post-translational regulation in response to infection, abiotic stress, and development (Gullner et al, 2018; Kumar and Trivedi, 2018; Sylvestre-Gonon et al, 2019). The list of abiotic stress factors affecting GST expression has recently expanded to UV-B, which induces the expression of 12 GSTUs including GSTU7 via class II TGA transcription factors (Herrera-Vásquez et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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GSTU7 affects growth performance and acts as an antagonist of oxidative stress induced by methyl viologen

Ugalde

Lamig

Herrera-Vásquez³

et al. 2020

Preprint

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Plant glutathione S-transferases (GSTs) are glutathione-dependent enzymes with versatile functions, mainly related to detoxification of electrophilic xenobiotics and peroxides. The Arabidopsis genome codes for 53 GSTs, divided into seven subclasses, however understanding of their precise functions is limited. A recent study showed that class II TGA transcription factors TGA2, TGA5 and TGA6 are essential for tolerance of UV-B-induced oxidative stress and that this tolerance is associated with an antioxidative function of cytosolic tau-class GSTUs. Specifically, TGA2 controls the expression of several GSTUs under UV-B light and constitutive expression of GSTU7 in the tga256 triple mutant is sufficient to revert the UV-B-susceptible phenotype of tga256. To further study the function of GSTU7, we characterized its role in mitigation of oxidative damage caused by the herbicide methyl viologen (MV). Under non-stress conditions, gstu7 null mutants were smaller than wild-type (WT) plants and delayed in the onset of the MV-induced antioxidative response, which led to accumulation of hydrogen peroxide and diminished seedling survival. Complementation of gstu7 by constitutively expressed GSTU7 rescued these phenotypes. Furthermore, live monitoring of the glutathione redox potential in intact cells with the fluorescent probe Grx1-roGFP2 revealed that GSTU7 overexpression completely abolished the MV-induced oxidation of the cytosolic glutathione buffer compared to WT plants. GSTU7 was found to act as a glutathione peroxidase able to complement the lack of peroxidase-type GSTs in yeast. Together, these findings show that GSTU7 is crucial in the antioxidative response by limiting oxidative damage and thus protecting cells from oxidative stress.

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“…The main plant ROS-processing systems are the well-recognized antioxidative enzymes that directly detoxify ROS in different organelles (superoxide dismutase SOD, catalase CAT, ascorbate peroxidase APX) and that regenerate reduced forms of redox buffers (monodehydroascorbate reductase MDAR, dehydroascorbate reductase DHAR, glutathione reductase GR, and NADPH-thioredoxin reductase NTR) (Meyer et al, 2012). Supporting the robustness of ROS metabolism in plants, ROS-processing systems also include other enzymes encoded by large multigene families, such as peroxiredoxins (PRX), glutaredoxins (GRX), thioredoxins (TRX), glutathione peroxidases (GPX) and glutathione transferases (GST) (Dixon and Edwards, 2010;Meyer et al, 2012;Bela et al, 2015;Liebthal et al, 2018;Sylvestre-Gonon et al, 2019). Interestingly, these thiol-based enzymes (PRX, GRX, TRX) and peroxidases (GPX and GST) are encoded by genes highly responsive to biotic and abiotic stress, and to a wide variety of stress-associated compounds such as Salicylic Acid (SA), Jasmonic Acid (JA), Abscisic Acid and H2O2 (Meyer et al, 2012;Das and Roychoudhury, 2014;Choudhury et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Transcription factor TGA2 is essential for UV-B stress tolerance controlling oxidative stress in Arabidopsis

Herrera-Vásquez

Fonseca

Ugalde

et al. 2020

Preprint

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Plants possess a diversity of Reactive Oxygen Species (ROS)-processing enzymes involved in sensing and controlling ROS levels under basal and stressful conditions. There is little information on the transcriptional regulators that control the expression of these ROSprocessing enzymes, particularly at the onset of the defense response to abiotic stress. Filling this gap, this paper reports a critical role for Arabidopsis TGA class II factors (TGA2, TGA5, and TGA6) in the tolerance response to UV-B light and photooxidative stress, by activating the expression of genes with antioxidative roles. We identified two clusters of genes responsive to UV-B and activated by TGA2/5/6 were identified using RNAseq and clustering analysis. The GSTU gene family, which encodes glutathione transferase enzymes from the Tau subclass, was overrepresented in these clusters. We corroborated the TGA2-mediated activation in response to UV-B for three model genes (GSTU7, GSTU8, and GSTU25) using RT-qPCR and ChIP analyses. Interestingly, using tga256 mutant and TGA2-and GSTU7complemented mutant plants, we demonstrated that TGA2-mediated induction of GSTU genes is essential to control ROS levels and oxidative damage after UV-B and MeV treatments. This evidence positions TGA class II factors, particularly TGA2, as a key players in the redox signaling network of Arabidopsis plants. 2 KeywordsArabidopsis, glutathione transferase, GSTU7, photooxidative stress, redox-signaling, ROS, TGA class II, TGA2, transcription factor, UV-B stress Running TitleTGA2 factor controls oxidative stress in UV-B stress Highlight:Arabidopsis TGA2 transcription factor is part of the redox-signaling network controlling ROS levels and oxidative damage in tolerance response to UV-B and photooxidative stress, via activation of antioxidant GSTU genes.

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Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases

Cited by 80 publications

References 176 publications

Target-Genes Reveal Species and Genotypic Specificity of Anthocyanin Pigmentation in Citrus and Related Genera

Target-Genes Reveal Species and Genotypic Specificity of Anthocyanin Pigmentation in Citrus and Related Genera

GSTU7 affects growth performance and acts as an antagonist of oxidative stress induced by methyl viologen

Transcription factor TGA2 is essential for UV-B stress tolerance controlling oxidative stress in Arabidopsis

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