Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties

Machałowski, Tomasz; Czajka, Maria; Petrenko, Iaroslav; Meißner, Heike; Schimpf, Christian; Rafaja, David; Ziętek, Jerzy; Dzięgiel, Beata; Adaszek, Łukasz; Voronkina, Alona; Kovalchuk, Valentin; Jaroszewicz, Joanna; Fursov, Andriy; Rahimi‐Nasrabadi, Mehdi; Stawski, Dawid; Bechmann, Nicole; Jesionowski, Teofil; Ehrlich, Hermann

doi:10.3390/md18060304

Cited by 12 publications

(9 citation statements)

References 65 publications

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“…Marine sponges are a remarkable donors of renewable biological materials [ 28 , 31 ]. Especially Verongida (Porifera) in particular was established as a good potential source of bioactive compounds (e.g., aeroplysinin-1 or isofistularin-3) [ 4 , 32 , 33 ] and chitinous skeletons [ 34 , 35 , 36 , 37 , 38 ]. Moreover, the possibility of underwater sponge farming makes them even more attractive [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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Tissue engineering (TE) is a field of regenerative medicine that has been experiencing a special boom in recent years. Among various materials used as components of 3D scaffolds, naturally formed chitinous materials seem to be especially attractive because of their abundance, non-toxic and eco-friendly character. In this study, chitinous skeleton isolated from the marine sponge Aplysina fistularis (phylum: Porifera) was used for the first time as a support for the cultivation of murine fibroblasts (Balb/3T3), human dermal fibroblasts (NHDF), human keratinocyte (HaCaT), and human neuronal (SH-SY5Y) cells. Characterization techniques such as ATR FTIR, TGA, and μCT, clearly indicate that an interconnected macro-porous, thermostable, pure α-chitin scaffold was obtained after alkali–acid treatment of air-dried marine sponge. The biocompatibility of the naturally formed chitin scaffolds was confirmed by cell attachment and proliferation determined by various microscopic methods (e.g., SEM, TEM, digital microscopy) and specific staining. Our observations show that fibroblasts and keratinocytes form clusters on scaffolds that resemble a skin structure, including the occurrence of desmosomes in keratinocyte cells. The results obtained here suggest that the chitinous scaffold from the marine sponge A. fistularis is a promising biomaterial for future research about tissues regeneration.

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Section: Introductionmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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“…They have been applied as pure materials or in combination with oxides (e.g., TiO 2 ), polymers, chitosan, etc. [ 7 , 14 , 20 , 23 , 24 , 25 , 26 , 27 ]. Attempts to enhance the germ-destroying or bacteriostatic efficacy of surfaces in recent times (since the beginning of the SARS-CoV-2 pandemic) are of great importance.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Antibacterial Metal Surfaces Using Magnetron-Sputtering Method

et al. 2021

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One-hundred-nanometer films consisting of silver, copper, and gold nanocrystallites were prepared, and their antibacterial properties were quantitatively measured. The magnetron-sputtering method was used for the preparation of the metallic films over the glass plate. Single- and double-layer films were manufactured. The films were thoroughly characterized with the XRD, SEM, EDS, and XPS methods. The antibacterial activity of the samples was investigated. Gram-negative Escherichia coli, strain K12 ATCC 25922 (E. coli), and Gram-positive Staphylococcus epidermidis, ATCC 49461 (S. epidermidis), were used in the microbial tests. The crystallite size was about 30 nm in the cases of silver and gold and a few nanometers in the case of copper. Significant oxidation of the copper films was proven. The antibacterial efficacy of the tested samples followed the order: Ag/Cu > Au/Cu > Cu. It was concluded that such metallic surfaces may be applied as contact-killing materials for a more effective fight against bacteria and viruses.

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“…In addition, chitin-based skeletal scaffolds from marine sponges covered with silver nanoparticles and silver-bromide exhibited excellent antibacterial ability. These scaffolds may be used in water filtration systems [ 309 ]. Apart from antimicrobial ability, hepcidins derived from the spotted scat Scatophagus argus also possess antiviral activities.…”

Section: Medical Applicationsmentioning

confidence: 99%

Biomaterials from the sea: Future building blocks for biomedical applications

Wan

Qin

Chen

et al. 2021

Bioactive Materials

105

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Marine resources have tremendous potential for developing high-value biomaterials. The last decade has seen an increasing number of biomaterials that originate from marine organisms. This field is rapidly evolving. Marine biomaterials experience several periods of discovery and development ranging from coralline bone graft to polysaccharide-based biomaterials. The latter are represented by chitin and chitosan, marine-derived collagen, and composites of different organisms of marine origin. The diversity of marine natural products, their properties and applications are discussed thoroughly in the present review. These materials are easily available and possess excellent biocompatibility, biodegradability and potent bioactive characteristics. Important applications of marine biomaterials include medical applications, antimicrobial agents, drug delivery agents, anticoagulants, rehabilitation of diseases such as cardiovascular diseases, bone diseases and diabetes, as well as comestible, cosmetic and industrial applications.

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Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties

Cited by 12 publications

References 65 publications

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

Fabrication of Antibacterial Metal Surfaces Using Magnetron-Sputtering Method

Biomaterials from the sea: Future building blocks for biomedical applications

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