Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties

Chaturvedi, Richa; Kang, Yumin; Eom, Yunji; Torati, Sri Ramulu; Kim, Cheol Gi

doi:10.3390/biology10100993

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…The high accumulation in the liver and spleen needs to be improved to increase drug delivery efficiency. Studies that reduce toxicity to normal cells by introducing polyethylene glycol (PEG) on the bacterial surface can be referred to, and as the introduction of PEG reduces the accumulation of the RES of nanoparticles, it needs to be studied in E. coli -based drug delivery systems [ 29 ]. The radioactivity observed in the bladder over a period of 1 day appears to be related to the clearance of 89 Zr-DFO dissociated from E. coli , considering the diameter of E. coli cells (1–2 µm) [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Long-Term Tumor-Targeting Effect of E. coli as a Drug Delivery System

Kim,

Lee,

Jeong

et al. 2024

Pharmaceuticals

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To overcome the limitations of current nano/micro-scale drug delivery systems, an Escherichia coli (E. coli)-based drug delivery system could be a potential alternative, and an effective tumor-targeting delivery system can be developed by attempting to perform chemical binding to the primary amine group of a cell membrane protein. In addition, positron emission tomography (PET) is a representative non-invasive imaging technology and is actively used in the field of drug delivery along with radioisotopes capable of long-term tracking, such as zirconium-89 (89Zr). The membrane proteins were labeled with 89Zr using chelate (DFO), and not only was the long-term biodistribution in tumors and major organs evaluated in the body, but the labeling stability of 89Zr conjugated to the membrane proteins was also evaluated through continuous tracking. E. coli accumulated at high levels in the tumor within 5 min (initial time) after tail intravenous injection, and when observed after 6 days, 89Zr-DFO on the surface of E. coli was found to be stable for a long period of time in the body. In this study, we demonstrated the long-term biodistribution and tumor-targeting effect of an E. coli-based drug delivery system and verified the in vivo stability of radioisotopes labeled on the surface of E. coli.

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Section: Resultsmentioning

confidence: 99%

Long-Term Tumor-Targeting Effect of E. coli as a Drug Delivery System

Kim,

Lee,

Jeong

et al. 2024

Pharmaceuticals

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“…The chemical conjugation of liposomes functionalized with reactive groups (-COOH) allows the covalent binding to primary amino groups (-NH2) intrinsic to bacterial cell membrane proteins. Another approach that bonds cargo to the primary groups of a MTB, more specifically to Magnetospirilium magneticum (AMB-1), was presented [11]. The authors used an MTB/PEG-biotin complex, corresponding to a covalent bond formation between bacteria and biotin-PEG-NHS polymer.…”

Section: Resultsmentioning

confidence: 99%

“…The primary desired characteristics are that bacteria should allow easy attachment of biomolecules, be able to control and provide easy characterization remotely. Many bacteria have been genetically engineered or chemically modified to fulfill these properties, including nanoliposomes attachment through amine coupling by activation of carboxylic acids with EDC/NHS chemistry [9], by NHS-ester amine coupling [10] or biotin-streptavidin reactions [11].…”

Section: Introductionmentioning

confidence: 99%

Azide click chemistry on magnetotactic bacteria: a versatile technique to attach a cargo

Rodriguez

Sirinelli-Kojadinovic

Rouzaud

et al. 2022

Preprint

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Adding biomolecules to living organisms and cells is the basis for creating living materials or biohybrids for robotic systems. Bioorthogonal chemistry allows covalently modifying biomolecules with functional groups not natively present under biological conditions and is therefore applicable to microorganisms and cells. Click chemistry is a biorthogonal chemistry approach that allows the study and manipulation of living entities. Incorporating the bioorthogonal click-chemistry handle, azide groups, into living microorganisms has been achieved by metabolic labeling, i.e., by culturing cells or organisms in a modified culture media having a specific natural molecular building block (e.g., amino acid, nucleotide, carbohydrate) modified with a tagged chemical analog. Here we explore the effect of the azide group incorporation into the magnetotactic bacteria Magnetospirillum gryphiswaldense (MSR-1) by adding a modified amino acid, 3-Azido-D-Alanine, during their cultivation. We show the existence of a concentration limit to effectively incorporate the azide group while maintaining the magnetic properties of the cells. We use this modification to explore the combination with versatile single-cell tagging methods.

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“…PEG is often referred to as a “stealth material” [ 220 ], due to its resistance to protein adsorption [ 17 , 39 , 40 ], and it generally resists cell attachment because of its higher affinity for water binding [ 221 ]. Regarding its tissue adhesion capability, little is known about PEG on its own.…”

Section: Polymersmentioning

confidence: 99%

Hydrogels in Cutaneous Wound Healing: Insights into Characterization, Properties, Formulation and Therapeutic Potential

Ribeiro,

Simões,

Vitorino

et al. 2024

Gels

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Hydrogels are polymeric materials that possess a set of characteristics meeting various requirements of an ideal wound dressing, making them promising for wound care. These features include, among others, the ability to absorb and retain large amounts of water and the capacity to closely mimic native structures, such as the extracellular matrix, facilitating various cellular processes like proliferation and differentiation. The polymers used in hydrogel formulations exhibit a broad spectrum of properties, allowing them to be classified into two main categories: natural polymers like collagen and chitosan, and synthetic polymers such as polyurethane and polyethylene glycol. This review offers a comprehensive overview and critical analysis of the key polymers that can constitute hydrogels, beginning with a brief contextualization of the polymers. It delves into their function, origin, and chemical structure, highlighting key sources of extraction and obtaining. Additionally, this review encompasses the main intrinsic properties of these polymers and their roles in the wound healing process, accompanied, whenever available, by explanations of the underlying mechanisms of action. It also addresses limitations and describes some studies on the effectiveness of isolated polymers in promoting skin regeneration and wound healing. Subsequently, we briefly discuss some application strategies of hydrogels derived from their intrinsic potential to promote the wound healing process. This can be achieved due to their role in the stimulation of angiogenesis, for example, or through the incorporation of substances like growth factors or drugs, such as antimicrobials, imparting new properties to the hydrogels. In addition to substance incorporation, the potential of hydrogels is also related to their ability to serve as a three-dimensional matrix for cell culture, whether it involves loading cells into the hydrogel or recruiting cells to the wound site, where they proliferate on the scaffold to form new tissue. The latter strategy presupposes the incorporation of biosensors into the hydrogel for real-time monitoring of wound conditions, such as temperature and pH. Future prospects are then ultimately addressed. As far as we are aware, this manuscript represents the first comprehensive approach that brings together and critically analyzes fundamental aspects of both natural and synthetic polymers constituting hydrogels in the context of cutaneous wound healing. It will serve as a foundational point for future studies, aiming to contribute to the development of an effective and environmentally friendly dressing for wounds.

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Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties

Cited by 6 publications

References 36 publications

Long-Term Tumor-Targeting Effect of E. coli as a Drug Delivery System

Long-Term Tumor-Targeting Effect of E. coli as a Drug Delivery System

Azide click chemistry on magnetotactic bacteria: a versatile technique to attach a cargo

Hydrogels in Cutaneous Wound Healing: Insights into Characterization, Properties, Formulation and Therapeutic Potential

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